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Literature summary extracted from

  • Morin, A.; Hummel, W.; Kula, M.R.
    Production of hydantoinase from Pseudomonas fluorescens strain DSM 84 (1986), Appl. Microbiol. Biotechnol., 25, 91-96.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.2 D-glucose
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O Pseudomonas fluorescens
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Pseudomonas putida
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Agrobacterium rhizogenes
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Agrobacterium tumefaciens
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Agrobacterium rubi
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Agrobacterium tumefaciens DSM 30147
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Agrobacterium rubi DSM 30149
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Agrobacterium rhizogenes DSM 30148
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Agrobacterium tumefaciens DSM 30150
-
 3-ureidopropionate
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas fluorescens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas putida
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium rhizogenes
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium tumefaciens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium rubi
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium tumefaciens DSM 30147
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium rubi DSM 30149
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium rhizogenes DSM 30148
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium tumefaciens DSM 30150
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium sp. DSM 30201
-
 N-carbamoyl glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Agrobacterium rhizogenes
-
 and strains DSM 30199, DSM 30200
-
3.5.2.2 Agrobacterium rhizogenes DSM 30148
-
 and strains DSM 30199, DSM 30200
-
3.5.2.2 Agrobacterium rubi
-
 and strains DSM 30149, DSM 30155
-
3.5.2.2 Agrobacterium rubi DSM 30149
-
 and strains DSM 30149, DSM 30155
-
3.5.2.2 Agrobacterium sp.
-
 and strain DSM 30203
-
3.5.2.2 Agrobacterium sp. DSM 30201
-
 and strain DSM 30203
-
3.5.2.2 Agrobacterium tumefaciens
-
 and strains DSM 30199, NRRL B-11291
-
3.5.2.2 Agrobacterium tumefaciens
-
 and strains DSM 30150, DSM 30205, DSM 30206, DSM 30207
-
3.5.2.2 Agrobacterium tumefaciens DSM 30147
-
 and strains DSM 30199, NRRL B-11291
-
3.5.2.2 Agrobacterium tumefaciens DSM 30150
-
 and strains DSM 30150, DSM 30205, DSM 30206, DSM 30207
-
3.5.2.2 Pseudomonas fluorescens
-
 strain DSM 84
-
3.5.2.2 Pseudomonas putida
-
 formerly classified as P. striata
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.2 0.005
-
 strain 30207, isopropylhydantoin as substrate Agrobacterium tumefaciens
3.5.2.2 0.006
-
 strain 30201, isopropylhydantoin as substrate Agrobacterium sp.
3.5.2.2 0.007
-
 strain 30205, isopropylhydantoin as substrate Agrobacterium tumefaciens
3.5.2.2 0.009
-
 strain 30199, isopropylhydantoin as substrate Agrobacterium tumefaciens
3.5.2.2 0.01
-
 strain 50106, isopropylhydantoin as substrate Pseudomonas fluorescens
3.5.2.2 0.011
-
 strain 2005, isopropylhydantoin as substrate Pseudomonas fluorescens
3.5.2.2 0.013
-
 strain 30150, isopropylhydantoin as substrate Agrobacterium tumefaciens
3.5.2.2 0.014
-
 strain 50106, incubated with hydantoin, isopropylhydantoin as substrate Pseudomonas fluorescens
3.5.2.2 0.014
-
 strain 50108, isopropylhydantoin as substrate Pseudomonas fluorescens
3.5.2.2 0.015
-
 strain 50090, incubated with hydantoin, isopropylhydantoin as substrate Pseudomonas fluorescens
3.5.2.2 0.016
-
 strain 11291, isopropylhydantoin as substrate Agrobacterium tumefaciens
3.5.2.2 0.016
-
 strain 50090, isopropylhydantoin as substrate Pseudomonas fluorescens
3.5.2.2 0.019
-
 strain 50154, isopropylhydantoin as substrate Pseudomonas fluorescens
3.5.2.2 0.021
-
 strain 30204, isopropylhydantoin as substrate Agrobacterium tumefaciens
3.5.2.2 0.022
-
 strain 2005, isopropylhydantoin as substrate Pseudomonas fluorescens
3.5.2.2 0.023
-
 strain 30147, isopropylhydantoin as substrate Agrobacterium tumefaciens
3.5.2.2 0.033
-
 strain 50108, isopropylhydantoin as substrate Pseudomonas fluorescens
3.5.2.2 0.033
-
 strain 30201, dihydrouracil as substrate Agrobacterium sp.
3.5.2.2 0.034
-
 strain 291, isopropylhydantoin as substrate Pseudomonas putida
3.5.2.2 0.038
-
 strain 84, isopropylhydantoin as substrate Pseudomonas fluorescens
3.5.2.2 0.049
-
 strain 549, isopropylhydantoin as substrate Pseudomonas putida
3.5.2.2 0.051
-
 strain 291, incubated with hydantoin, isopropylhydantoin as substrate Pseudomonas putida
3.5.2.2 0.059
-
 strain 11291, dihydrouracil as substrate Agrobacterium tumefaciens
3.5.2.2 0.094
-
 strain 11291, incubated with hydantoin Agrobacterium tumefaciens
3.5.2.2 0.112
-
 strain 30205, dihydrouracil as substrate Agrobacterium tumefaciens
3.5.2.2 0.127
-
 strain 549, incubated with hydantoin, isopropylhydantoin as substrate Pseudomonas putida
3.5.2.2 0.128
-
 strain 30150, dihydrouracil as substrate Agrobacterium tumefaciens
3.5.2.2 0.15
-
 strain 2005, dihydrouracil as substrate Pseudomonas fluorescens
3.5.2.2 0.185
-
 strain 84, incubated with hydantoin, isopropylhydantoin as substrate Pseudomonas fluorescens
3.5.2.2 0.19
-
 strain 50154, dihydrouracil as substrate Pseudomonas fluorescens
3.5.2.2 0.193
-
 strain 30204, dihydrouracil as substrate Agrobacterium tumefaciens
3.5.2.2 0.194
-
 strain 50090, dihydrouracil as substrate Pseudomonas fluorescens
3.5.2.2 0.212
-
 strain 30147, dihydrouracil as substrate Agrobacterium tumefaciens
3.5.2.2 0.299
-
 strain 30155, dihydrouracil as substrate Agrobacterium rubi
3.5.2.2 0.31
-
 strain 50106, dihydrouracil as substrate Pseudomonas fluorescens
3.5.2.2 0.317
-
 strain 30208, dihydrouracil as substrate Agrobacterium tumefaciens
3.5.2.2 0.425
-
 strain 30207, dihydrouracil as substrate Agrobacterium tumefaciens
3.5.2.2 0.5
-
 strain 291, dihydrouracil as substrate Pseudomonas putida
3.5.2.2 0.53
-
 strain 50108, dihydrouracil as substrate Pseudomonas fluorescens
3.5.2.2 0.562
-
 strain 30206, dihydrouracil as substrate Agrobacterium tumefaciens
3.5.2.2 0.62
-
 strain 2005, incubated with hydantoin, dihydrouracil as substrate Pseudomonas fluorescens
3.5.2.2 0.73
-
 strain 549, incubated with hydantoin, dihydrouracil as substrate Pseudomonas putida
3.5.2.2 0.78
-
 dihydrouracil as substrate Pseudomonas fluorescens
3.5.2.2 0.81
-
 strain 50154, incubated with hydantoin, dihydrouracil as substrate Pseudomonas fluorescens
3.5.2.2 0.98
-
 strain 50108, incubated with hydantoin, dihydrouracil as substrate Pseudomonas fluorescens
3.5.2.2 1.11
-
 strain 50106, incubated with hydantoin, dihydrouracil as substrate Pseudomonas fluorescens
3.5.2.2 1.14
-
 strain 30155, dihydrouracil as substrate Agrobacterium rubi
3.5.2.2 1.2
-
 strain 549, dihydrouracil as substrate Pseudomonas putida
3.5.2.2 1.4
-
 strain 50090, incubated with hydantoin, dihydrouracil as substrate Pseudomonas fluorescens
3.5.2.2 1.9
-
 strain 549, incubated with hydantoin, dihydrouracil as substrate Pseudomonas putida
3.5.2.2 2.25
-
 incubated with hydantoin Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
3.5.2.2 4°C, potassium phosphate buffer at pH 8 permits best enzyme recovery and assured stability of enzyme for more than 14 days Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas fluorescens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas putida 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium rhizogenes 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium tumefaciens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium sp. 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium rubi 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium tumefaciens DSM 30147 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium rubi DSM 30149 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium rhizogenes DSM 30148 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium tumefaciens DSM 30150 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium sp. DSM 30201 3-ureidopropionate
-
 ?
3.5.2.2 5-(p-hydroxybenzyl)hydantoin + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl-D-methionine steric configuration D ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium tumefaciens DSM 30147 N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium tumefaciens DSM 30150 N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium sp. DSM 30201 N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Agrobacterium tumefaciens DSM 30147 N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Agrobacterium tumefaciens DSM 30150 N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Agrobacterium sp. DSM 30201 N-carbamoyl-D-valine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium rhizogenes N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium rubi N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium tumefaciens DSM 30147 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium rubi DSM 30149 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium rhizogenes DSM 30148 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium tumefaciens DSM 30150 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium sp. DSM 30201 N-carbamoyl glycine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.2 microbial hydantoinase
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.2 27
-
-
 Pseudomonas fluorescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.2 40
-
 stable at temperatures up to, starts to lose significant activity at 50°C, remaining activity at 55°C less than 25%, heat treatment in presence of substrate stabilizes enzyme at temperatures in excess of 40°C Pseudomonas fluorescens
3.5.2.2 51
-
 more than 40% activity lost after 10 min Pseudomonas fluorescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.2 7.1 7.2 more stable in slightly alkaline buffers Pseudomonas fluorescens