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Literature summary extracted from

  • Gofshtein-Gandman, L.V.; Keynan, A.; Milner, Y.
    Bacteria of the genus Bacillus have a hydrolase stereospecific to the D isomer of benzoyl-arginine-p-nitroanilide (1988), J. Bacteriol., 170, 5895-5900.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.72 2-mercaptoethanol no effect Bacillus subtilis
3.5.1.72 2-mercaptoethanol slight activation Bacillus cereus
3.5.1.72 dithiothreitol no effect Bacillus subtilis
3.5.1.72 dithiothreitol slight activation Bacillus cereus

General Stability

EC Number General Stability Organism
3.5.1.72 Ca2+ decreases thermostability Bacillus subtilis
3.5.1.72 Mg2+ decreases thermostability Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.72 iodoacetamide
-
 Bacillus cereus
3.5.1.72 iodoacetamide no inhibition Bacillus subtilis
3.5.1.72 leupeptin peptide inhibitor Bacillus cereus
3.5.1.72 leupeptin peptide inhibitor Bacillus subtilis
3.5.1.72 additional information no inhibition by EDTA Bacillus cereus
3.5.1.72 additional information no inhibition by EDTA; strain 168, no inhibition by lysozyme, 2 mg/ml, or cell wall lysate Bacillus subtilis
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide substrate inhibition at concentration above 1 mM Bacillus cereus
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide substrate inhibition at concentration above 0.5 mM Bacillus subtilis
3.5.1.72 p-chloromercuribenzene sulfonic acid 80% inhibition Bacillus cereus
3.5.1.72 p-chloromercuribenzene sulfonic acid no inhibition Bacillus subtilis
3.5.1.72 p-chloromercuribenzoate
-
 Bacillus cereus
3.5.1.72 p-chloromercuribenzoate no inhibition Bacillus subtilis
3.5.1.72 tosyl-L-Arg methyl ester
-
 Bacillus cereus
3.5.1.72 tosyl-L-Arg methyl ester
-
 Bacillus subtilis
3.5.1.72 tosyl-L-Lys chloromethyl ketone
-
 Bacillus cereus
3.5.1.72 tosyl-L-Lys chloromethyl ketone
-
 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.72 0.029
-
 N-benzoyl-D-Arg 4-nitroanilide strain 168 Bacillus subtilis
3.5.1.72 0.14
-
 N-benzoyl-D-Arg 4-nitroanilide
-
 Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.72 cytoplasm 80-90% in soluble form in cytoplasm Bacillus subtilis 5737
-
3.5.1.72 cytoplasm 80-90% in soluble form in cytoplasm Bacillus cereus 5737
-
3.5.1.72 intracellular
-
 Bacillus subtilis 5622
-
3.5.1.72 intracellular
-
 Bacillus cereus 5622
-
3.5.1.72 additional information strain 168, subcellular distribution, no activity in cell wall lysate and membranes Bacillus subtilis
-
-
3.5.1.72 protoplast
-
 Bacillus subtilis
-
-
3.5.1.72 protoplast
-
 Bacillus cereus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.72 CaCl2 activation Bacillus subtilis
3.5.1.72 CaCl2 activation Bacillus cereus
3.5.1.72 CaCl2 optimum concentration: 1 mM Bacillus cereus
3.5.1.72 CaCl2 strain 168, optimal concentration: 5 mM Bacillus subtilis
3.5.1.72 MgCl2 activation, optimal concentration: 5 mM Bacillus subtilis
3.5.1.72 MgCl2 activation, optimal concentration: 5 mM Bacillus cereus
3.5.1.72 NaCl 100 mM: two- to threefold activation Bacillus subtilis
3.5.1.72 NaCl 100 mM: two- to threefold activation Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.72 66000
-
 gel filtration and PAGE Bacillus subtilis
3.5.1.72 66000
-
 gel filtration and PAGE Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.72 Bacillaceae
-
 found in all aerobic and anaerobic members of the family tested
-
3.5.1.72 Bacillus cereus
-
 T
-
3.5.1.72 Bacillus cereus T
-
 T
-
3.5.1.72 Bacillus subtilis
-
 168 trpC2 and nonspore-forming mutant 43.4 spo0A43 leu-8
-
3.5.1.72 Bacillus thuringiensis
-
 var. israelensis
-
3.5.1.72 Clostridium sp.
-
-
-
3.5.1.72 Lysinibacillus sphaericus
-
 2362
-
3.5.1.72 Lysinibacillus sphaericus 2362
-
 2362
-
3.5.1.72 marine aerobic sporeforming bacterium 6A10
-
-
-
3.5.1.72 no activity in Acetobacter xylinum
-
-
-
3.5.1.72 no activity in Corynebacterium bovis
-
-
-
3.5.1.72 no activity in Escherichia coli
-
-
-
3.5.1.72 no activity in Escherichia coli JM83
-
-
-
3.5.1.72 no activity in Streptococcus mutans serotype C
-
-
-
3.5.1.72 no activity in Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.72 partial Bacillus cereus
3.5.1.72 strain 168, partial Bacillus subtilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.72 additional information enzyme is more active in vegetative cells than in germinating spores Bacillus subtilis
-
3.5.1.72 additional information enzyme is more active in vegetative cells than in germinating spores Lysinibacillus sphaericus
-
3.5.1.72 additional information enzyme is more active in vegetative cells than in germinating spores Bacillus cereus
-
3.5.1.72 additional information enzyme is more active in vegetative cells than in germinating spores Clostridium sp.
-
3.5.1.72 additional information enzyme is more active in vegetative cells than in germinating spores Bacillaceae
-
3.5.1.72 additional information enzyme is more active in vegetative cells than in germinating spores Bacillus thuringiensis
-
3.5.1.72 additional information enzyme is more active in vegetative cells than in germinating spores marine aerobic sporeforming bacterium 6A10
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.72 additional information
-
-
 Bacillus subtilis
3.5.1.72 additional information
-
-
 Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.72 additional information ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Bacillus subtilis ?
-
 ?
3.5.1.72 additional information ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Lysinibacillus sphaericus ?
-
 ?
3.5.1.72 additional information ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Bacillus cereus ?
-
 ?
3.5.1.72 additional information ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Clostridium sp. ?
-
 ?
3.5.1.72 additional information ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Bacillaceae ?
-
 ?
3.5.1.72 additional information ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Bacillus thuringiensis ?
-
 ?
3.5.1.72 additional information ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Lysinibacillus sphaericus 2362 ?
-
 ?
3.5.1.72 additional information ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Bacillus cereus T ?
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Bacillus subtilis N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Lysinibacillus sphaericus N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Bacillus cereus N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Clostridium sp. N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Bacillaceae N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Bacillus thuringiensis N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer Bacillus subtilis N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer Lysinibacillus sphaericus N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer Bacillus cereus N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer Clostridium sp. N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer Bacillaceae N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer Bacillus thuringiensis N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer marine aerobic sporeforming bacterium 6A10 N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Lysinibacillus sphaericus 2362 N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer Lysinibacillus sphaericus 2362 N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium Bacillus cereus T N-benzoyl-D-Arg + 4-nitroaniline
-
 ?
3.5.1.72 N-benzoyl-D-Arg-4-nitroanilide + H2O D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer Bacillus cereus T N-benzoyl-D-Arg + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.72 45
-
-
 Bacillus subtilis
3.5.1.72 45
-
-
 Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.72 additional information
-
 strain 168, Ca2+ and Mg2+ ions decrease thermostability Bacillus subtilis
3.5.1.72 60
-
 strain 168, 2 min, 40% loss of activity in 0.05 M Tris buffer, pH 7.8, 60% loss of activity with 5 mM MgCl2, 80% loss of activity with 5 mM CaCl2 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.72 7.8
-
 assay at Bacillus subtilis
3.5.1.72 7.8
-
 assay at Lysinibacillus sphaericus
3.5.1.72 7.8
-
 assay at Bacillus cereus
3.5.1.72 7.8
-
 assay at Clostridium sp.
3.5.1.72 7.8
-
 assay at Bacillaceae
3.5.1.72 7.8
-
 assay at Bacillus thuringiensis
3.5.1.72 7.8
-
 assay at marine aerobic sporeforming bacterium 6A10