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Literature summary extracted from

  • Asakura, M; Nagahashi, Y.; Hamada, M.; Kawai, M.; Kadobayashi, K.; Narahara, M.; Nakagawa, S.; Kawai, Y.; Hama, T.; Miyake, M.
    Purification and properties of beta-citryl-L-glutamate-hydrolysing enzyme from rat testis particulate (1995), Biochim. Biophys. Acta, 1250, 35-42.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.68 Lubrol Px enzyme solubilized, activation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.68 AMP no or slight inhibition Rattus norvegicus
3.5.1.68 ATP 0.1 mM: 78% inhibition, 1 mM: 97% inhibition; strong competitive inhibition Rattus norvegicus
3.5.1.68 citrate 0.1 mM: 77% inhibition, 1 mM: 98% inhibition Rattus norvegicus
3.5.1.68 EDTA inhibition completely reversed by Mn2+, activity 30% restored by Ca2+ and 13% restored by Mg2+ Rattus norvegicus
3.5.1.68 EGTA
-
 Rattus norvegicus
3.5.1.68 glutamate 0.1 mM: 34% inhibition, 1 mM: 81% inhibition Rattus norvegicus
3.5.1.68 GMP no or slight inhibition Rattus norvegicus
3.5.1.68 GTP 0.1 mM: 90% inhibition, 1 mM: 99% inhibition; strong competitive inhibition Rattus norvegicus
3.5.1.68 L-Quisqualate 0.1 mM: 80% inhibition, 1 mM: 96% inhibition; non-competitive potent inhibitor Rattus norvegicus
3.5.1.68 additional information no or very slight inhibition by kinate, N-methyl-D-aspartate Rattus norvegicus
3.5.1.68 nucleotides strong inhibition Rattus norvegicus
3.5.1.68 phosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.68 0.0025
-
 beta-Citryl-L-glutamate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.68 membrane localized in particulate fractions Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.68 Ca2+ required for full activity, can replace Mn2+ to some extent Rattus norvegicus
3.5.1.68 Divalent metal ions required Rattus norvegicus
3.5.1.68 Fe2+ no activation Rattus norvegicus
3.5.1.68 Mg2+ required for full activity, can replace Mn2+ to some extent Rattus norvegicus
3.5.1.68 Mn2+ required for full activity Rattus norvegicus
3.5.1.68 Mn2+ maximal activity at 0.5-1 mM Rattus norvegicus
3.5.1.68 additional information enzyme is a metalloenzyme Rattus norvegicus
3.5.1.68 additional information no activation by Ni2+, Zn2+ Rattus norvegicus
3.5.1.68 additional information no activation by Cu2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.68 350000
-
 PAGE, after digestion with N-glycosidase F Rattus norvegicus
3.5.1.68 380000
-
 gel filtration Rattus norvegicus
3.5.1.68 420000
-
 PAGE, before digestion with N-glycosidase F Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.68 Rattus norvegicus
-
 Wistar
-
3.5.1.68 Rattus norvegicus Wistar
-
 Wistar
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.68 glycoprotein
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.68 solubilized by 0.5% Lubrol PX, most effective detergent Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.68 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.68 0.0192
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.68 beta-citryl-L-glutamate + H2O deacylation of beta-citryl-L-glutamate Rattus norvegicus citrate + L-glutamate
-
 ir
3.5.1.68 beta-citryl-L-glutamate + H2O best substrate, high substrate specificity for beta-citryl-L-glutamate Rattus norvegicus citrate + L-glutamate
-
 ir
3.5.1.68 beta-citryl-L-glutamine + H2O at about 1/30 of that for beta-citryl-L-glutamate Rattus norvegicus citrate + L-glutamine
-
 ir
3.5.1.68 additional information substrate specificity Rattus norvegicus ?
-
 ?
3.5.1.68 additional information little or no activity with N-formyl-L-aspartate, N-formyl-L-glutamine, N-acetyl-L-glutamate, alpha-citryl-L-glutamate, alpha- and beta-citryl-alpha-L-glutamylglutamate Rattus norvegicus ?
-
 ?
3.5.1.68 additional information no activity with N-acetyl-alpha-aspartylglutamate Rattus norvegicus ?
-
 ?
3.5.1.68 additional information little or no activity with N-acetyl-L-glutamate Rattus norvegicus ?
-
 ?
3.5.1.68 additional information substrate specificity Rattus norvegicus Wistar ?
-
 ?
3.5.1.68 additional information little or no activity with N-formyl-L-aspartate, N-formyl-L-glutamine, N-acetyl-L-glutamate, alpha-citryl-L-glutamate, alpha- and beta-citryl-alpha-L-glutamylglutamate Rattus norvegicus Wistar ?
-
 ?
3.5.1.68 additional information no activity with N-acetyl-alpha-aspartylglutamate Rattus norvegicus Wistar ?
-
 ?
3.5.1.68 additional information little or no activity with N-acetyl-L-glutamate Rattus norvegicus Wistar ?
-
 ?
3.5.1.68 N-acetyl-L-aspartate + H2O poor substrate Rattus norvegicus acetate + L-aspartate
-
 ?
3.5.1.68 N-formyl-L-glutamate + H2O poor substrate Rattus norvegicus formate + L-glutamate
-
 ?
3.5.1.68 N-formyl-L-glutamate + H2O poor substrate Rattus norvegicus Wistar formate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.68 multimer composed of 90000, 100000, 115000 and 130000 Da subunits, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.68 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.68 additional information
-
 pI of about pH 4.3 Rattus norvegicus
3.5.1.68 6.5 7 in the presence of Mn2+ Rattus norvegicus