EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.65 | L-Malic acid | activation, 0.7 mM: 167%, 3.3 mM: 227%, 8 mM: 230%, 13.3 mM: 235% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | additional information | D-malic acid, 8 mM, no effect | Camellia sinensis |
EC Number | General Stability | Organism |
---|---|---|
3.5.1.65 | enzyme very unstable | Camellia sinensis |
3.5.1.65 | theanine hydrolase activity increases slightly during first 10 h after plucking but thereafter decreased gradually | Camellia sinensis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.65 | acetic acid | 8 mM: 88% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | acidic amino acid | - |
Camellia sinensis | |
3.5.1.65 | beta-Alanine | partial inhibition, 8 mM: 89% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | glycine | 8 mM: 99% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | L-alanine | strong inhibition, 0.7 mM: 67%, 3.3 mM: 50%, 8 mM: 45%, 53.3 mM: 23% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | L-asparagine | strong inhibition, 8 mM: 76% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | L-aspartic acid | strong inhibition, 8 mM: 53% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | L-Citric acid | 8 mM: 95% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | L-Glutamic acid | strong inhibition, 0.7 mM: 86%, 3.3 mM: 58%, 8 mM: 56%, 53.3 mM: 25% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | L-glutamine | strong inhibition, 8 mM: 75% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | L-leucine | partial inhibition, 8 mM: 89% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | L-serine | partial inhibition, 8 mM: 95% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | Maleic acid | 8 mM: 98% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | additional information | D-alanine, 8 mM, and D-malic acid, 8 mM: no effect | Camellia sinensis | |
3.5.1.65 | oxaloacetic acid | strong inhibition, 8 mM: 59% of activity compared to H2O | Camellia sinensis | |
3.5.1.65 | succinic acid | strong inhibition, 8 mM: 77% of activity compared to H2O | Camellia sinensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.65 | additional information | potassium ion and phosphate ion: no effect | Camellia sinensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.65 | N5-ethyl-L-glutamine + H2O | Camellia sinensis | L-theanine, gamma-glutamylethylamide, principal component of taste of tea | L-glutamate + ethylamine | - |
? | |
3.5.1.65 | N5-ethyl-L-glutamine + H2O | Camellia sinensis | part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants | L-glutamate + ethylamine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.65 | Camellia sinensis | - |
tea | - |
3.5.1.65 | Camellia sinensis | - |
cv. Yabukita | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.65 | partial, glutaminase could not be separated | Camellia sinensis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.1.65 | leaf | - |
Camellia sinensis | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.65 | additional information | - |
- |
Camellia sinensis |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.1.65 | 4°C, 45% loss of activity within 1 day | Camellia sinensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.65 | additional information | substrate specificity | Camellia sinensis | ? | - |
? | |
3.5.1.65 | additional information | also acts on other N-alkyl-L-glutamines | Camellia sinensis | ? | - |
? | |
3.5.1.65 | additional information | no activity with N-methylpropionamide and N-ethylpropionamide | Camellia sinensis | ? | - |
? | |
3.5.1.65 | N5-ethyl-L-glutamine + H2O | L-theanine, gamma-glutamylethylamide, principal component of taste of tea | Camellia sinensis | L-glutamate + ethylamine | - |
? | |
3.5.1.65 | N5-ethyl-L-glutamine + H2O | part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants | Camellia sinensis | L-glutamate + ethylamine | - |
? | |
3.5.1.65 | N5-isobutyl-L-glutamine + H2O | gamma-glutamyl-isobutylamide, 136% of activity compared to L-theanine | Camellia sinensis | L-glutamate + isobutylamine | - |
? | |
3.5.1.65 | N5-methyl-L-glutamine + H2O | gamma-glutamylmethylamide, 77% of activity compared to L-theanine | Camellia sinensis | L-glutamate + methylamine | - |
? | |
3.5.1.65 | N5-n-butyl-L-glutamine + H2O | gamma-glutamyl-n-butylamide, 276% of activity compared to L-theanine | Camellia sinensis | L-glutamate + n-butylamine | - |
? | |
3.5.1.65 | N5-n-pentyl-L-glutamine + H2O | gamma-glutamyl-n-amylamide, 211% of activity compared to L-theanine | Camellia sinensis | L-glutamate + n-pentylamine | - |
? | |
3.5.1.65 | N5-n-propyl-L-glutamine + H2O | gamma-glutamyl-n-propylamide, 144% of activity compared to theanine | Camellia sinensis | L-glutamate + n-propylamine | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.65 | 8.5 | - |
- |
Camellia sinensis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.65 | 6.5 | 9 | pH 6.5: about 22% of maximal activity, pH 9.0: about 95% of maximal activity | Camellia sinensis |
3.5.1.65 | 7.5 | - |
about half of maximum activity at pH 7.5 | Camellia sinensis |