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Literature summary extracted from

  • Clemens, D.L.; Lee, B.Y.; Horwitz, M.A.
    Purification, characterization, and genetic analysis of Mycobacterium tuberculosis urease, a potentially critical determinant of host-pathogen interaction (1995), J. Bacteriol., 177, 5644-5652.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.5 urease gene cluster Mycobacterium tuberculosis

General Stability

EC Number General Stability Organism
3.5.1.5 resistant to denaturation by 4 M urea Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.5 Acetohydroxamate
-
 Mycobacterium tuberculosis
3.5.1.5 EDTA no inhibition Mycobacterium tuberculosis
3.5.1.5 Hydroxyurea
-
 Mycobacterium tuberculosis
3.5.1.5 NEM
-
 Mycobacterium tuberculosis
3.5.1.5 phenylphosphoramidate
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 0.3
-
 Urea
-
 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.5 Nickel contains about 6 Ni2+ ions per urease molecule Mycobacterium tuberculosis
3.5.1.5 Nickel nickel metalloenzyme Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.5 11000
-
 x + 11000 + x * 11000 + x * 62000, SDS-PAGE Mycobacterium tuberculosis
3.5.1.5 62000
-
 x + 11000 + x * 11000 + x * 62000, SDS-PAGE Mycobacterium tuberculosis
3.5.1.5 185000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.5 urea + H2O Mycobacterium tuberculosis expression increases 10fold upon nitrogen deprivation, the enzyme is involved in nitrogen aquisition by the bacterium ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.5
-
 Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.5 101
-
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 Mycobacterium tuberculosis CO2 + NH3
-
 ?
3.5.1.5 urea + H2O expression increases 10fold upon nitrogen deprivation, the enzyme is involved in nitrogen aquisition by the bacterium Mycobacterium tuberculosis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.5 ? x + 11000 + x * 11000 + x * 62000, SDS-PAGE Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.5 60
-
 30 min, stable up to Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.5 7.2
-
-
 Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.5 5.5 8 pH 5.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity Mycobacterium tuberculosis