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Literature summary extracted from

  • Mahadevan, S.; Sauer, F.D.; Erfle, J.D.
    Purification and properties of urease from bovine rumen (1977), Biochem. J., 163, 495-501.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.5 EDTA slight stimulation Bos taurus

General Stability

EC Number General Stability Organism
3.5.1.5 dithiothreitol, 50 mM, stabilizes Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.5 Acetohydroxamate reversible Bos taurus
3.5.1.5 Ba2+
-
 Bos taurus
3.5.1.5 Ca2+
-
 Bos taurus
3.5.1.5 Cd2+
-
 Bos taurus
3.5.1.5 Cu2+
-
 Bos taurus
3.5.1.5 Hg2+
-
 Bos taurus
3.5.1.5 Hydroxyurea reversible Bos taurus
3.5.1.5 Mg2+
-
 Bos taurus
3.5.1.5 Mn2+
-
 Bos taurus
3.5.1.5 NEM 0.1 mM, complete inhibition Bos taurus
3.5.1.5 Ni2+
-
 Bos taurus
3.5.1.5 p-chloromercuribenzene sulfonate 0.1 mM, complete inhibition Bos taurus
3.5.1.5 Sr2+
-
 Bos taurus
3.5.1.5 Zn2+
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 0.83
-
 Urea
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.5 125000
-
 gel filtration Bos taurus
3.5.1.5 130000
-
 sucrose density gradient centrifugation Bos taurus
3.5.1.5 135000
-
 non-denaturing PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.5
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.5 rumen
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.5 59.9
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 Bos taurus CO2 + NH3
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.5 8
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.5 7 8.5 pH 7.0: 52% of maximal activity, pH 8.5: 42% of maximal activity Bos taurus