Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Skouloubris, S.; Labigne, A.; de Reuse H.
    The AmiE aliphatic amidase and AmiF formamidase of Helicobacter pylori: natural evolution of two enzyme paralogues (2001), Mol. Microbiol., 40, 596-609.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.49
-
Helicobacter pylori
3.5.1.49 expression in Escherichia coli Helicobacter pylori

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.49 C166A no enzymic activity Helicobacter pylori
3.5.1.49 C166S no enzymic activity Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.49 iodoacetate 2.5 mM, 55% inhibition Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.49 32
-
Formamide pH 7.4, 30°C Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.49 37300
-
4 * 3400, SDS-PAGE, 4 * 37300, deduced from gene sequence Helicobacter pylori
3.5.1.49 160000
-
gel filtration Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.49 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.49 recombinant protein Helicobacter pylori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.49 cell culture
-
Helicobacter pylori
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.49 1040
-
pH 7.4, 30°C Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.49 formamide + H2O from AmiF gene, that exclusively hydrolyzes formamide, involved in nitrogen metabolism Helicobacter pylori formate + NH3
-
?
3.5.1.49 formamide + H2O unique and specific substrate Helicobacter pylori formate + NH3
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.49 homotetramer 4 * 3400, SDS-PAGE, 4 * 37300, deduced from gene sequence Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.49 45
-
-
Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.49 6
-
-
Helicobacter pylori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.49 5 9
-
Helicobacter pylori