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Literature summary extracted from

  • Friedrich, C.G.; Mitrenga, G.
    Utilization of aliphatic amides and formation of two different amidases by Alcaligenes eutrophus (1981), J. Gen. Microbiol., 125, 367-374.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.50 pentanamide + H2O Cupriavidus necator
-
?
-
?
3.5.1.50 pentanamide + H2O Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.49 Cupriavidus necator
-
-
-
3.5.1.50 Cupriavidus necator
-
-
-
3.5.1.50 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.49 cell culture induction of enzyme synthesis using formamide- or acetamide-containing media Cupriavidus necator
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.50 0.014
-
substrate: pentanamide Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.49 formamide + H2O
-
Cupriavidus necator formate + NH3
-
?
3.5.1.49 formamide + H2O
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 formate + NH3
-
?
3.5.1.50 aliphatic carboxylic acid amides + H2O acts more slowly on other short-chain aliphatic amides Cupriavidus necator carboxylic acid + NH3
-
?
3.5.1.50 aliphatic carboxylic acid amides + H2O acts more slowly on other short-chain aliphatic amides Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 carboxylic acid + NH3
-
?
3.5.1.50 pentanamide + H2O
-
Cupriavidus necator pentanoate + NH3
-
?
3.5.1.50 pentanamide + H2O
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 pentanoate + NH3
-
?
3.5.1.50 pentanamide + H2O
-
Cupriavidus necator ?
-
?
3.5.1.50 pentanamide + H2O
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.50 8
-
assay at Cupriavidus necator