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Literature summary extracted from

  • Imai, T.
    Purification and properties of nicotinamide mononucleotide amidohydrolase from Azotobacter vinelandii (1973), J. Biochem., 73, 139-153.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.42 ATP weak activator Azotobacter vinelandii
3.5.1.42 diphosphate weak activator Azotobacter vinelandii
3.5.1.42 phosphate weak activator Azotobacter vinelandii

General Stability

EC Number General Stability Organism
3.5.1.42 DTT required during purification Azotobacter vinelandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.42 alpha-nicotinic acid adenine dinucleotide non-competitive inhibitor, Ki: 8 mM Azotobacter vinelandii
3.5.1.42 alpha-nicotinic acid mononucleotide non-competitive inhibitor, Ki: 21 mM Azotobacter vinelandii
3.5.1.42 beta-nicotinic acid adenine dinucleotide non-competitive inhibitor, Ki: 7 mM Azotobacter vinelandii
3.5.1.42 Cu2+ strong inhibitor Azotobacter vinelandii
3.5.1.42 Isonicotinic acid non-competitive inhibitor, Ki: 8.3 mM Azotobacter vinelandii
3.5.1.42 nicotinic acid non-competitive inhibitor, Ki: 4.4 mM Azotobacter vinelandii
3.5.1.42 p-chloromercuribenzoate
-
Azotobacter vinelandii
3.5.1.42 Picolinic acid non-competitive inhibitor, Ki: 9.8 mM Azotobacter vinelandii
3.5.1.42 quinolinic acid non-competitive inhibitor, Ki: 3.9 mM Azotobacter vinelandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.42 1.05
-
beta-nicotinamide D-ribonucleotide
-
Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.42 Mg2+ significant stimulation Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.42 25700 26300 gel filtration Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.42 beta-nicotinamide D-ribonucleotide + H2O Escherichia coli
-
beta-nicotinate D-ribonucleotide + NH3
-
?
3.5.1.42 beta-nicotinamide D-ribonucleotide + H2O Azotobacter vinelandii involvement in NAD metabolism beta-nicotinate D-ribonucleotide + NH3
-
ir
3.5.1.42 nicotinamide ribonucleoside + H2O Azotobacter vinelandii 14% activity compared to nicotinamide D-ribonucleotide nicotinic acid ribonucleoside + NH3
-
ir

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.42 Azotobacter vinelandii
-
-
-
3.5.1.42 Escherichia coli
-
-
-
3.5.1.42 no activity in Oryctolagus cuniculus
-
-
-
3.5.1.42 no activity in Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.42 to homogeneity, chromatography techniques Azotobacter vinelandii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.42 cell culture
-
Escherichia coli
-
3.5.1.42 cell culture
-
Azotobacter vinelandii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.42 0.22
-
-
Azotobacter vinelandii

Storage Stability

EC Number Storage Stability Organism
3.5.1.42 4°C, Tris-HCl, pH 7.5, 1 week Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.42 beta-nicotinamide D-ribonucleotide + H2O
-
Escherichia coli beta-nicotinate D-ribonucleotide + NH3
-
?
3.5.1.42 beta-nicotinamide D-ribonucleotide + H2O
-
Azotobacter vinelandii beta-nicotinate D-ribonucleotide + NH3
-
ir
3.5.1.42 beta-nicotinamide D-ribonucleotide + H2O involvement in NAD metabolism Azotobacter vinelandii beta-nicotinate D-ribonucleotide + NH3
-
ir
3.5.1.42 nicotinamide ribonucleoside + H2O 14% activity compared to nicotinamide D-ribonucleotide Azotobacter vinelandii nicotinic acid ribonucleoside + NH3
-
ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.42 64
-
-
Azotobacter vinelandii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.42 64
-
preincubation without substrate: complete denaturation within 32 min Azotobacter vinelandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.42 6.8 7.2
-
Azotobacter vinelandii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.42 3.9
-
quinolinic acid
-
Azotobacter vinelandii
3.5.1.42 4.4
-
nicotinic acid
-
Azotobacter vinelandii
3.5.1.42 7
-
beta-nicotinic acid adenine dinucleotide
-
Azotobacter vinelandii
3.5.1.42 8
-
alpha-nicotinic acid adenine dinucleotide
-
Azotobacter vinelandii
3.5.1.42 8.3
-
Isonicotinic acid
-
Azotobacter vinelandii
3.5.1.42 9.8
-
Picolinic acid
-
Azotobacter vinelandii
3.5.1.42 21
-
alpha-nicotinic acid mononucleotide
-
Azotobacter vinelandii