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Literature summary extracted from

  • Farnaud, S.; Tata, Renee.; Sohi, M.K.; Wan, P.R.; Sutton, B.J.
    Evidence that cysteine-166 is the active-site nucleophile of Pseudomonas aeruginasa amidase: crystallization and preliminary X-ray diffraction analysis of the enzyme (1999), Biochem. J., 340, 711-714.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.4 expression in Escherichia coli JM109 Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.4 hanging-drop technique at both 16 and 4°C, rhombohedral morphology Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.4 C166A mutant catalytically inactive Pseudomonas aeruginosa
3.5.1.4 C166S mutant catalytically inactive Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 38441
-
 alpha = beta = gamma = 75°, and space group, R3 or R32, quaternary structure determinated by gel filtration and SDS-PAGE, 6 * 38441 deduced from primary structure Pseudomonas aeruginosa
3.5.1.4 38441
-
 6 * 38441, calculated from primary structure Pseudomonas aeruginosa
3.5.1.4 215000
-
 PAGE Pseudomonas aeruginosa
3.5.1.4 221000
-
 gel filtration Sephacryl S300HR Pseudomonas aeruginosa
3.5.1.4 221000
-
 gel filtration on Sephracryl S300HR Pseudomonas aeruginosa
3.5.1.4 224000
-
 gel filtration Sephacryl S400HR Pseudomonas aeruginosa
3.5.1.4 224000
-
 gel filtration on Sephracryl S400HR Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Escherichia coli
-
 harboring pALJ30 and pALJ20
-
3.5.1.4 Escherichia coli JM109
-
 harboring pALJ30 and pALJ20
-
3.5.1.4 Pseudomonas aeruginosa
-
 C166S and C166A mutants
-
3.5.1.4 Pseudomonas aeruginosa
-
 mutant strain KLAM59
-

Subunits

EC Number Subunits Comment Organism
3.5.1.4 hexamer with most probably 32 symmetry, crystals of both wild-type and C166S amidase grow with identical, rhombohedral morphology. X-ray diffraction analysis established the unit cell dimensions, a = b = c = 84 A Pseudomonas aeruginosa
3.5.1.4 hexamer alpha = beta = gamma = 75°, and space group, R3 or R32, quaternary structure determinated by gel filtration and SDS-PAGE, 6 * 38441 deduced from primary structure Pseudomonas aeruginosa
3.5.1.4 hexamer 6 * 38441, calculated from primary structure Pseudomonas aeruginosa