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Literature summary extracted from
- A comparative study of acquired amidase activity in Pseudomaonas species (1986), J. Gen. Microbiol., 132, 2185-2204.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.4 | iodoacetamide | - |
Pseudomonas aeruginosa |  |
| 3.5.1.4 | iodoacetamide | no effect of 2-chloropropionamide | Pseudomonas putida | |
| 3.5.1.4 | N-ethylmaleimide | inhibition at 10 mM, C-amidase | Pseudomonas putida | |
| 3.5.1.4 | p-hydroxymercuribenzoate | - |
Pseudomonas aeruginosa | |
| 3.5.1.4 | p-hydroxymercuribenzoate | inhibition at 10 mM, inhibits the C-amidase | Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.4 | 80 | - |
2-Chloropropionamide | A-amidase | Pseudomonas putida | |
| 3.5.1.4 | 100 | - |
acetamide | A-amidase | Pseudomonas putida | |
| 3.5.1.4 | 206 | - |
2-Chloropropionamide | strain PPW3, C-amidase | Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 59000 | - |
strain PPW3, C-amidase, determined by gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.4 | 2-chloropropionamide + H2O | Pseudomonas putida | - |
2-chloropropionate + NH3 | - |
? | |
| 3.5.1.4 | 2-chloropropionamide + H2O | Pseudomonas putida PP3 | - |
2-chloropropionate + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.4 | Pseudomonas aeruginosa | - |
PAU3 and PAU5 | - |
| 3.5.1.4 | Pseudomonas putida | - |
and strain PPW3 | - |
| 3.5.1.4 | Pseudomonas putida PP3 | - |
and strain PPW3 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.4 | strain PPW3, C-amidase, ammonium sulfate fractionation, gel filtration, DEAE-Sephadex, preparative PAGE, purification factor 40 | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 0.00003 | - |
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PP3 | Pseudomonas putida |
| 3.5.1.4 | 0.00024 | - |
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PP3 | Pseudomonas putida |
| 3.5.1.4 | 0.00027 | - |
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PP3 | Pseudomonas putida |
| 3.5.1.4 | 0.0017 | - |
growth substrate propionamide, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU3 | Pseudomonas aeruginosa |
| 3.5.1.4 | 0.019 | - |
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PPW3 | Pseudomonas putida |
| 3.5.1.4 | 0.023 | - |
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PPW3 | Pseudomonas putida |
| 3.5.1.4 | 0.031 | - |
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5 | Pseudomonas putida |
| 3.5.1.4 | 0.039 | - |
growth substrate propionamide, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5 | Pseudomonas putida |
| 3.5.1.4 | 0.078 | - |
A-amidase, substrate was 2-chloropropionamide | Pseudomonas putida |
| 3.5.1.4 | 0.12 | - |
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PPW3 | Pseudomonas putida |
| 3.5.1.4 | 0.14 | - |
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5 | Pseudomonas putida |
| 3.5.1.4 | 0.25 | - |
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PAU5 | Pseudomonas putida |
| 3.5.1.4 | 0.914 | - |
strain PPW, activity of C-amidase after preparative PAGE, final yield 17% of original activity | Pseudomonas putida |
| 3.5.1.4 | 1.89 | - |
strain PPW3, C-amidase, substrate 2-chloropropionamide | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.4 | 2-chloropropionamide + H2O | - |
Pseudomonas putida | 2-chloropropionate + NH3 | - |
? | |
| 3.5.1.4 | 2-chloropropionamide + H2O | - |
Pseudomonas putida PP3 | 2-chloropropionate + NH3 | - |
? | |
| 3.5.1.4 | acetamide + H2O | - |
Pseudomonas putida | acetate + NH3 | - |
? | |
| 3.5.1.4 | acetamide + H2O | - |
Pseudomonas putida PP3 | acetate + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.4 | More | strain PPW3, C-amidase, subunit molecular mass of 29000 Da, SDS-PAGE | Pseudomonas putida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.4 | A-amidase | specificity towards aliphatic amides | Pseudomonas aeruginosa |
| 3.5.1.4 | C-amidase | specificity towards chlorinated aliphatic amides | Pseudomonas putida |
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