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Literature summary extracted from
- Characterization of an inducible amidase from Pseudomonas acidovorans AE1 (1975), Eur. J. Biochem., 53, 357-369.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.4 | 4-chloromercuribenzoic acid | as -SH-blocking reagent, substrate 4-nitroacetanilide, incubation 10 min, 10 micromol 4-chloromercuribenzoic acid, residual activity 4.8%, activity restored by addition of 1 mM cysteine, 1 h incubation 67% restored | Delftia acidovorans |  |
| 3.5.1.4 | acetanilide | substrate 4-nitroacetanilide, Ki: 14 micromol, competitive inhibition | Delftia acidovorans | |
| 3.5.1.4 | Diethyl-4-nitrophenyl phosphate | - |
Delftia acidovorans | |
| 3.5.1.4 | diisopropylphosphorofluoridate | - |
Delftia acidovorans | |
| 3.5.1.4 | Hg2+ | HgCl2 as -SH-blocking reagent, incubation 10 min, 10 micromol HgCl2, 81% of activity restored by addition of 1 mM cysteine | Delftia acidovorans | |
| 3.5.1.4 | iodoacetamide | concentration 0.2 M at pH 8.0 | Delftia acidovorans | |
| 3.5.1.4 | o-Nitrophenol | competitive inhibition | Delftia acidovorans | |
| 3.5.1.4 | Phenacetin | substrate 4-nitroacetanilide, Ki: 8.9 nM, competitive inhibition, induction of activity for 2-nitrophenylacetate and methylbutyrate | Delftia acidovorans | |
| 3.5.1.4 | Phenetidine | uncompetitive inhibition | Delftia acidovorans | |
| 3.5.1.4 | physostigmine sulfate | - |
Delftia acidovorans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.4 | 0.006 | - |
4-nitrophenylacetate | - |
Delftia acidovorans | |
| 3.5.1.4 | 0.014 | - |
acetanilide | - |
Delftia acidovorans | |
| 3.5.1.4 | 0.029 | - |
phenylacetate | - |
Delftia acidovorans | |
| 3.5.1.4 | 0.042 | - |
2-nitroacetanilide | - |
Delftia acidovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 55300 | - |
amino acid composition | Delftia acidovorans |
| 3.5.1.4 | 56700 | - |
SDS-PAGE | Delftia acidovorans |
| 3.5.1.4 | 57500 | - |
gel filtration | Delftia acidovorans |
| 3.5.1.4 | 59100 | - |
reaction of purified enzyme with diethyl-4-nitrophenyl phosphate | Delftia acidovorans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.4 | 2-nitroacetanilide | Delftia acidovorans | - |
2-nitrophenylamine + acetate | - |
? | |
| 3.5.1.4 | 2-nitroacetanilide | Delftia acidovorans AE1 | - |
2-nitrophenylamine + acetate | - |
? | |
| 3.5.1.4 | 4-nitrophenyl acetate + H2O | Delftia acidovorans | - |
4-nitrophenol + acetate | - |
? | |
| 3.5.1.4 | 4-nitrophenyl acetate + H2O | Delftia acidovorans AE1 | - |
4-nitrophenol + acetate | - |
? | |
| 3.5.1.4 | phenetidine + acetate | Delftia acidovorans | transferase activity | phenacetin + H2O | - |
? | |
| 3.5.1.4 | phenetidine + acetate | Delftia acidovorans AE1 | transferase activity | phenacetin + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.4 | Delftia acidovorans | - |
- |
- |
| 3.5.1.4 | Delftia acidovorans AE1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.4 | lysis of crude extract, addition of streptomycin, ammonium sulfate fractionation, desalting by Sephadex-gel filtration, DEAE-Sepharose chromatography, hydroxyapatite column, yield 72% | Delftia acidovorans |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.5.1.4 | a monocarboxylic acid amide + H2O = a monocarboxylate + NH3 | hydrolysis of ester and aromatic amide | Delftia acidovorans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 0.05 | - |
substrate acetamide, crude extract | Delftia acidovorans |
| 3.5.1.4 | 7.7 | - |
substrate 4-nitroacetanilide, crude extract | Delftia acidovorans |
| 3.5.1.4 | 73 | - |
substrate phenacetin, pure enzyme, pH 8.6 and 30°C | Delftia acidovorans |
| 3.5.1.4 | 121 | - |
substrate 2-nitroacetanilide, pure enzyme, pH 8.6 and 30°C | Delftia acidovorans |
| 3.5.1.4 | 133 | - |
substrate acetanilide, pure enzyme, pH 8.6 and 30°C | Delftia acidovorans |
| 3.5.1.4 | 149 | - |
substrate phenylacetate, pure enzyme, pH 8.6 and 30°C | Delftia acidovorans |
| 3.5.1.4 | 181 | - |
substrate 4-nitrophenylacetate, pure enzyme, pH 8.6 and 30°C | Delftia acidovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.4 | 2-nitroacetanilide | - |
Delftia acidovorans | 2-nitrophenylamine + acetate | - |
? | |
| 3.5.1.4 | 2-nitroacetanilide | - |
Delftia acidovorans AE1 | 2-nitrophenylamine + acetate | - |
? | |
| 3.5.1.4 | 4-nitrophenyl acetate + H2O | - |
Delftia acidovorans | 4-nitrophenol + acetate | - |
? | |
| 3.5.1.4 | 4-nitrophenyl acetate + H2O | - |
Delftia acidovorans AE1 | 4-nitrophenol + acetate | - |
? | |
| 3.5.1.4 | acetanilide + H2O | - |
Delftia acidovorans | acetate + aniline | - |
? | |
| 3.5.1.4 | acetanilide + H2O | - |
Delftia acidovorans AE1 | acetate + aniline | - |
? | |
| 3.5.1.4 | p-nitrophenylacetamide + H2O | - |
Delftia acidovorans | p-nitrophenylacetic acid + NH3 | - |
? | |
| 3.5.1.4 | phenetidine + acetate | transferase activity | Delftia acidovorans | phenacetin + H2O | - |
? | |
| 3.5.1.4 | phenetidine + acetate | transferase activity | Delftia acidovorans AE1 | phenacetin + H2O | - |
? | |
| 3.5.1.4 | phenylacetamide | - |
Delftia acidovorans | phenylacetic acid + NH3 | - |
? | |
| 3.5.1.4 | phenylacetamide | - |
Delftia acidovorans AE1 | phenylacetic acid + NH3 | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.4 | 129 | - |
2-nitroacetanilide | - |
Delftia acidovorans | |
| 3.5.1.4 | 134 | - |
acetanilide | - |
Delftia acidovorans | |
| 3.5.1.4 | 144 | - |
4-nitrophenylacetate | - |
Delftia acidovorans | |
| 3.5.1.4 | 147 | - |
phenylacetate | - |
Delftia acidovorans | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 10 | - |
- |
Delftia acidovorans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 7 | 9 | - |
Delftia acidovorans |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.4 | 0.0000089 | - |
Phenacetin | substrate 4-nitroacetanilide, competitive inhibition, induction of activity for 2-nitrophenylacetate and methylbutyrate | Delftia acidovorans | |
| 3.5.1.4 | 0.0014 | - |
acetanilide | substrate 4-nitroacetanilide, competitive inhibition | Delftia acidovorans | |
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