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Literature summary extracted from

  • Ciskanik, L.M.; Wilczek, J.M.; Fallon, R.D.
    Purification and characterization of an enantioselective amidase from Pseudomonas chlororaphis B23 (1995), Appl. Environ. Microbiol., 61, 998-1003.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.4 synthesis acrylamide production Pseudomonas chlororaphis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 Ag+ preincubation for 30 min, remaining activity in presence of 1 mM DTT Pseudomonas chlororaphis
3.5.1.4 Cd2+ preincubated for 30 min Pseudomonas chlororaphis
3.5.1.4 Cu2+ preincubated for 30 min, remaining activity in presence of 1 mM DTT Pseudomonas chlororaphis
3.5.1.4 DTNB preincubated for 30 min, remaining activity in presence of 1 mM DTT Pseudomonas chlororaphis
3.5.1.4 Fe2+ preincubated for 30 min Pseudomonas chlororaphis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 54000
-
 2 * 54000, SDS-PAGE Pseudomonas chlororaphis
3.5.1.4 105000
-
-
 Pseudomonas chlororaphis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.4 propionamide + H2O Pseudomonas chlororaphis
-
 propionic acid + NH3
-
 ?
3.5.1.4 propionamide + H2O Pseudomonas chlororaphis B23
-
 propionic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Pseudomonas chlororaphis
-
-
-
3.5.1.4 Pseudomonas chlororaphis B23
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4 ammonium sulfate precipitation, 30 to 55%, and hydrophobic, anion-exchange, gel filtration, and ceramic hydroxyapatite chromatography steps, DTT required to maintain enzymatic activity, overall level of purification 114-fold, recovery rate 21% Pseudomonas chlororaphis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 17.7
-
 turnover propionamide to propionate and ammonia Pseudomonas chlororaphis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 propionamide + H2O
-
 Pseudomonas chlororaphis propionic acid + NH3
-
 ?
3.5.1.4 propionamide + H2O
-
 Pseudomonas chlororaphis B23 propionic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 dimer 2 * 54000, SDS-PAGE Pseudomonas chlororaphis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.4 10.5
-
 Propionamide
-
 Pseudomonas chlororaphis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.4 3.4 11 100% activity at pH values between 5.9 and 9.9 Pseudomonas chlororaphis
3.5.1.4 7 8.6 against the substrate propionamide Pseudomonas chlororaphis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.4 3.9 9.9 in various buffer Pseudomonas chlororaphis