Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Davidson, L.; Brear, D.R.; Wingard, P.; Hawkins, J.; Kitto, G.B.
    Purification and properties of an L-glutaminase-L-asparaginase from Pseudomonas acidovorans (1977), J. Bacteriol., 129, 1379-1386.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.38 p-chloromercuribenzoate 26% inhibition at 0.1 mM Delftia acidovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.38 0.015
-
 L-asparagine
-
 Delftia acidovorans
3.5.1.38 0.022
-
 L-glutamine
-
 Delftia acidovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.38 39000
-
 4 * 39000, SDS-PAGE Delftia acidovorans
3.5.1.38 156000
-
 gel filtration Delftia acidovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.38 L-asparagine + H2O Delftia acidovorans hydrolysed at a rate of 0.83 compared to L-glutamine L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O Delftia acidovorans
-
 L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.38 Delftia acidovorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.38 to homogeneity, 3step chromatography Delftia acidovorans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.38 cell culture
-
 Delftia acidovorans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.38 86
-
 asparaginase activity Delftia acidovorans
3.5.1.38 104
-
 glutaminase activity Delftia acidovorans

Storage Stability

EC Number Storage Stability Organism
3.5.1.38 4°C, 50 mM borate buffer, pH 7.0, 100 mM NaCl, 1 mM EDTA, 3 to 4 months Delftia acidovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.38 beta-D-aspartyl hydroxylammonium sulfate + H2O
-
 Delftia acidovorans L-Asp + hydroxylammonium sulfate
-
 ?
3.5.1.38 beta-L-aspartyl hydroxylammonium sulfate + H2O
-
 Delftia acidovorans L-Asp + hydroxylammonium sulfate
-
 ?
3.5.1.38 D-asparagine + H2O low activity Delftia acidovorans D-aspartate + NH3
-
 ?
3.5.1.38 D-glutamine + H2O low activity Delftia acidovorans D-glutamate + NH3
-
 ?
3.5.1.38 gamma-L-glutamyl hydroxylammonium sulfate + H2O
-
 Delftia acidovorans L-glutamate + hydroxylammonium sulfate
-
 ?
3.5.1.38 L-asparagine + H2O hydrolysed at a rate of 0.83 compared to L-glutamine Delftia acidovorans L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O
-
 Delftia acidovorans L-glutamate + NH3
-
 ?
3.5.1.38 N-acetyl-L-asparagine + H2O 5% of the activity compared to L-asparagine Delftia acidovorans N-acetyl-L-aspartate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.38 tetramer 4 * 39000, SDS-PAGE Delftia acidovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.38 50
-
 half-life: 10 min, protection against thermal inactivation by addition of substrate Delftia acidovorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.38 7.4
-
 70% activity Delftia acidovorans