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Literature summary extracted from

  • Ramakrishnan, M.S.; Joseph, R.
    Characterization of an extracellular asparaginase of Rhodosporidium toruloides CBS14 exhibiting unique physicochemical properties (1996), Can. J. Microbiol., 42, 316-325.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.38 2-mercaptoethanol slight activation Rhodotorula toruloides
3.5.1.38 cysteine slight activation Rhodotorula toruloides
3.5.1.38 dithiothreitol slight activation Rhodotorula toruloides

General Stability

EC Number General Stability Organism
3.5.1.38 stabilizing effect by addition of glycine and maintaining the pH to 9.0 Rhodotorula toruloides

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.38 2-Hydroxy-5-nitrobenzyl bromide 40% inhibition at 10 mM, concentration-dependent, protection against inhibition in presence of substrate Rhodotorula toruloides
3.5.1.38 Hg2+ slight inhibition Rhodotorula toruloides
3.5.1.38 iodoacetamide slight inhibition Rhodotorula toruloides
3.5.1.38 N-bromosuccinimide concentration-dependent inhibition, 40% at 10 mM, protection against inhibition in presence of substrate Rhodotorula toruloides
3.5.1.38 p-chloromercuribenzoate slight inhibition Rhodotorula toruloides
3.5.1.38 Urea 95% loss of activity in 2.25 M Rhodotorula toruloides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.38 1.43
-
 L-asparagine
-
 Rhodotorula toruloides
3.5.1.38 2.4
-
 N-glycyl-L-asparagine
-
 Rhodotorula toruloides
3.5.1.38 6.45
-
 L-glutamine
-
 Rhodotorula toruloides
3.5.1.38 8
-
 N-carbamoyl-L-asparagine
-
 Rhodotorula toruloides
3.5.1.38 10
-
 N-acetyl-L-asparagine
-
 Rhodotorula toruloides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.38 periplasmic space
-
 Rhodotorula toruloides 42597
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.38 Ni2+ slightly enhanced activity Rhodotorula toruloides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.38 87000
-
 2 * 87000, SDS-PAGE Rhodotorula toruloides
3.5.1.38 180000
-
 gel filtration Rhodotorula toruloides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.38 L-asparagine + H2O Rhodotorula toruloides
-
 L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O Rhodotorula toruloides one fifth of the activity on L-asparagine L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.38 Rhodotorula toruloides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.38 to homogeneity, chromatography, preparative gel electrophoresis Rhodotorula toruloides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.38 cell culture
-
 Rhodotorula toruloides
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.38 2156
-
 glutaminase activity Rhodotorula toruloides
3.5.1.38 10510
-
 asparaginase activity Rhodotorula toruloides

Storage Stability

EC Number Storage Stability Organism
3.5.1.38 -10°C, lyophilized to dryness in the presence of 50 mM Tris, 250 mM glycine, pH 9.0, 90% activity in 3 months Rhodotorula toruloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.38 beta-L-aspartyl hydroxylammonium sulfate + H2O
-
 Rhodotorula toruloides L-Asp + hydroxylammonium sulfate
-
 ?
3.5.1.38 gamma-L-glutamyl hydroxylammonium sulfate + H2O
-
 Rhodotorula toruloides L-glutamate + hydroxylammonium sulfate
-
 ?
3.5.1.38 L-asparagine + H2O
-
 Rhodotorula toruloides L-aspartate + NH3
-
 ?
3.5.1.38 L-asparagine + H2O not active on D-asparagine Rhodotorula toruloides L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O one fifth of the activity on L-asparagine Rhodotorula toruloides L-glutamate + NH3
-
 ?
3.5.1.38 N-acetyl-L-asparagine + H2O
-
 Rhodotorula toruloides N-acetyl-L-aspartate + NH3
-
 ?
3.5.1.38 N-carbamoyl-L-asparagine + H2O
-
 Rhodotorula toruloides N-carbamoyl-L-aspartate + NH3
-
 ?
3.5.1.38 N-glycyl-L-asparagine + H2O 60% higher activity than on L-asparagine Rhodotorula toruloides N-glycyl-L-aspartate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.38 dimer 2 * 87000, SDS-PAGE Rhodotorula toruloides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.38 37
-
-
 Rhodotorula toruloides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.38 5 7 optimal range for asparaginase activity Rhodotorula toruloides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.38 7
-
 complete inactivation Rhodotorula toruloides