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Literature summary extracted from

  • Nandakumar, R.; Wakayama, M.; Nagano, Y.; Kawamura, T.; Sakai, K.; Moriguchi, M.
    Overexpression of salt-tolerant glutaminase from Micrococcus luteus K-3 in Escherichia coli and its purification (1999), Protein Expr. Purif., 15, 155-161.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.2 overexpression in Escherichia coli Micrococcus luteus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.2
-
 Micrococcus luteus

General Stability

EC Number General Stability Organism
3.5.1.2 stabilized by MgCl2 and glycerol in addition to NaCl Micrococcus luteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.2 EDTA
-
 Micrococcus luteus
3.5.1.2 NaCIO4 0.3 M, 25% inhibition Micrococcus luteus
3.5.1.2 NaH2PO4 0.3 M, complete inhibition Micrococcus luteus
3.5.1.2 NaI above 0.3 M Micrococcus luteus
3.5.1.2 NaNO3 0.3 M, 15% inhibition Micrococcus luteus
3.5.1.2 PCMB
-
 Micrococcus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.2 KCl 2 M, 1.4fold activation Micrococcus luteus
3.5.1.2 NaBr 0.25 M, 1.2-1.3fold activation Micrococcus luteus
3.5.1.2 NaF 0.25 M, 1.2-1.3fold activation Micrococcus luteus
3.5.1.2 NaI 0.25 M, 1.2-1.3fold activation Micrococcus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.2 43000
-
 x * 43000, SDS-PAGE Micrococcus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.2 L-Gln + H2O Micrococcus luteus
-
 L-Glu + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Micrococcus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.2 recombinant enzyme Micrococcus luteus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.2 1.23
-
 recombinant enzyme Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 Gln + H2O
-
 Micrococcus luteus Glu + NH3
-
 ?
3.5.1.2 L-Gln + H2O
-
 Micrococcus luteus L-Glu + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.2 ? x * 43000, SDS-PAGE Micrococcus luteus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.2 50
-
-
 Micrococcus luteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.2 50
-
 in presence of 1.71 M NaCl, stable up to Micrococcus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.2 8
-
-
 Micrococcus luteus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.2 7.5 9 stable Micrococcus luteus