Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hartman, S.C.
    Glutaminases and gamma-glutamyltransferases (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 79-100.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.2 1,10-phenanthroline slight activation Escherichia coli
3.5.1.2 dithiothreitol slight activation Escherichia coli
3.5.1.2 EDTA slight activation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.2 6-diazo-5-oxo-L-norleucine
-
 Escherichia coli
3.5.1.2 Ag+
-
 Escherichia coli
3.5.1.2 Cu2+
-
 Escherichia coli
3.5.1.2 Hg2+ 0.1 mM, complete inhibition Escherichia coli
3.5.1.2 p-mercuribenzoate 0.1 mM, complete inhibition, presence of Gln prevents inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.2 additional information
-
 additional information effect of pH on Km-value Escherichia coli
3.5.1.2 0.42
-
 Gln
-
 Escherichia coli
3.5.1.2 2.9
-
 Glu
-
 Escherichia coli
3.5.1.2 3.3
-
 gamma-glutamyl methylamide
-
 Escherichia coli
3.5.1.2 10
-
 gamma-thiomethyl glutamate
-
 Escherichia coli
3.5.1.2 12
-
 gamma-glutamyl hydrazide
-
 Escherichia coli
3.5.1.2 23
-
 gamma-thioethyl glutamate
-
 Escherichia coli
3.5.1.2 50
-
 gamma-ethyl glutamate
-
 Escherichia coli
3.5.1.2 64
-
 gamma-methyl glutamate
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Escherichia coli
-
 production of significant amounts of enzyme only after the stage of rapid exponential growth
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.2
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.2 L-glutamine + H2O = L-glutamate + NH3 mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 gamma-ethyl glutamate + H2O
-
 Escherichia coli Glu + ethanol
-
 ?
3.5.1.2 gamma-ethyl glutamate + hydroxylamine
-
 Escherichia coli gamma-glutamyl hydroxamate + ethanol
-
 ?
3.5.1.2 gamma-glutamyl-hydrazide + H2O
-
 Escherichia coli Glu + hydroxyhydrazine
-
 ?
3.5.1.2 gamma-glutamyl-hydrazide + hydroxylamine
-
 Escherichia coli Glu + hydroxyhydrazine
-
 ?
3.5.1.2 gamma-glutamyl-hydroxamate + H2O
-
 Escherichia coli Glu + hydroxylamine
-
 ?
3.5.1.2 gamma-glutamyl-hydroxamate + hydroxylamine
-
 Escherichia coli gamma-glutamyl hydroxamate + hydroxylamine
-
 ?
3.5.1.2 gamma-glutamyl-methoxyamide + H2O
-
 Escherichia coli Glu + N-hydroxy-O-methylhydroxylamine
-
 ?
3.5.1.2 gamma-glutamyl-methoxyamide + hydroxylamine
-
 Escherichia coli gamma-glutamyl hydroxamate + N-hydroxy-O-methylhydroxylamine
-
 ?
3.5.1.2 gamma-glutamyl-methylamide + H2O
-
 Escherichia coli Glu + N-methylhydroxylamine
-
 ?
3.5.1.2 gamma-glutamyl-methylamide + hydroxylamine
-
 Escherichia coli gamma-glutamyl hydroxamate + N-methylhydroxylamine
-
 ?
3.5.1.2 gamma-methyl glutamate + H2O
-
 Escherichia coli Glu + methanol
-
 r
3.5.1.2 gamma-methyl glutamate + hydroxylamine
-
 Escherichia coli gamma-glutamyl hydroxamate + methanol
-
 ?
3.5.1.2 gamma-thioethyl glutamate + H2O
-
 Escherichia coli Glu + thioethanol
-
 ?
3.5.1.2 gamma-thioethyl glutamate + hydroxylamine
-
 Escherichia coli gamma-glutamyl hydroxamate + ?
-
 ?
3.5.1.2 gamma-thiomethyl glutamate + H2O
-
 Escherichia coli Glu + thiomethanol
-
 ?
3.5.1.2 gamma-thiomethyl glutamate + hydroxylamine
-
 Escherichia coli gamma-glutamyl hydroxamate + ?
-
 ?
3.5.1.2 Gln + H2O
-
 Escherichia coli Glu + NH3
-
 ?
3.5.1.2 Glu + hydroxylamine
-
 Escherichia coli gamma-glutamyl hydroxamate + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.2 additional information
-
 additional information effect of pH on turnover number Escherichia coli
3.5.1.2 8
-
 gamma-glutamyl methylamide
-
 Escherichia coli
3.5.1.2 14
-
 gamma-glutamyl hydrazide
-
 Escherichia coli
3.5.1.2 36
-
 gamma-ethyl glutamate
-
 Escherichia coli
3.5.1.2 212
-
 gamma-glutamyl-hydroxamate
-
 Escherichia coli
3.5.1.2 296
-
 gamma-glutamyl methoxyamide
-
 Escherichia coli
3.5.1.2 300
-
 gamma-thioethyl glutamate
-
 Escherichia coli
3.5.1.2 645
-
 gamma-methyl glutamate
-
 Escherichia coli
3.5.1.2 1260
-
 gamma-thiomethyl glutamate
-
 Escherichia coli
3.5.1.2 1270
-
 Gln
-
 Escherichia coli
3.5.1.2 5080
-
 Glu
-
 Escherichia coli