Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • D'Adamo, A.F.; Peisach, J.; Manner, G.; Weiler, C.T.
    N-Acetyl-aspartate amidohydrolase: Purification and properties (1977), J. Neurochem., 28, 739-744.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.5.1.15 pure enzyme extremely unstable Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.15 aspartic acid 0.002 M, 20-25% inhibition Rattus norvegicus
3.5.1.15 glutamic acid 0.002 M, 20-25% inhibition Rattus norvegicus
3.5.1.15 N-ethylmaleimide
-
 Rattus norvegicus
3.5.1.15 p-hydroxymercuribenzoate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.15 0.2
-
 N-acyl L-aspartic acid
-
 Sus scrofa
3.5.1.15 0.2
-
 N-acyl L-aspartic acid brain enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.15 cytosol
-
 Rattus norvegicus 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.15 N-acetyl-L-aspartic acid + H2O Rattus norvegicus
-
 acetate + aspartic acid
-
 ?
3.5.1.15 N-acetyl-L-aspartic acid + H2O Sus scrofa
-
 acetate + aspartic acid
-
 ?
3.5.1.15 N-acetyl-L-aspartic acid + H2O Gallus sp.
-
 acetate + aspartic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.15 Gallus sp.
-
-
-
3.5.1.15 Rattus norvegicus
-
-
-
3.5.1.15 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.15 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.15 brain
-
 Rattus norvegicus
-
3.5.1.15 brain
-
 Sus scrofa
-
3.5.1.15 brain
-
 Gallus sp.
-
3.5.1.15 kidney
-
 Rattus norvegicus
-
3.5.1.15 kidney
-
 Sus scrofa
-
3.5.1.15 kidney
-
 Gallus sp.
-
3.5.1.15 liver
-
 Rattus norvegicus
-
3.5.1.15 liver
-
 Gallus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.15 0.47
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.5.1.15 -20°C, frozen storage of undialyzed enzyme for a few days leads to a complete disappearance of activity following a single thaw Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.15 additional information N-methyl aspartic acid, N-carbamoyl aspartic acid and N-glycyl aspartic acids are no substrates Rattus norvegicus ?
-
 ?
3.5.1.15 N-acetyl-L-aspartic acid + H2O
-
 Rattus norvegicus acetate + aspartic acid
-
 ?
3.5.1.15 N-acetyl-L-aspartic acid + H2O
-
 Sus scrofa acetate + aspartic acid
-
 ?
3.5.1.15 N-acetyl-L-aspartic acid + H2O
-
 Gallus sp. acetate + aspartic acid
-
 ?
3.5.1.15 N-acyl L-aspartic acid + H2O
-
 Rattus norvegicus acetate + L-aspartate
-
 ?
3.5.1.15 N-acyl L-aspartic acid + H2O
-
 Sus scrofa acetate + L-aspartate
-
 ?
3.5.1.15 N-acyl L-aspartic acid + H2O
-
 Gallus sp. acetate + L-aspartate
-
 ?
3.5.1.15 N-formyl aspartic acid + H2O
-
 Rattus norvegicus formate + aspartate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.15 N-acetyl-aspartate deacetylase
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.15 57
-
 rapidly denatures above Rattus norvegicus
3.5.1.15 70
-
 stable at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.15 7.5 8.5
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.15 3 10
-
 Rattus norvegicus