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Literature summary extracted from

  • Wriston, J.C.
    L-Asparaginase (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 101-121.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.1.1 medicine enzyme has antitumor activity Cavia porcellus
3.5.1.1 medicine enzyme has antitumor activity Escherichia coli
3.5.1.1 medicine enzyme has antitumor activity Serratia marcescens
3.5.1.1 medicine enzyme has antitumor activity Pectobacterium carotovorum
3.5.1.1 medicine enzyme has antitumor activity Erwinia aroidea

General Stability

EC Number General Stability Organism
3.5.1.1 stable to dialysis in the cold, but labile under conditions which promote surface denaturation Cavia porcellus
3.5.1.1 stable to repeated freezing and thawing Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 5-Diazo-4-oxo-L-norvaline
-
 Escherichia coli
3.5.1.1 Dimethylsulfoxide
-
 Escherichia coli
3.5.1.1 Hg2+
-
 Cavia porcellus
3.5.1.1 NH4+ product inhibition at pH 8.5 Escherichia coli
3.5.1.1 p-mercuribenzoate
-
 Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 additional information
-
 additional information
-
 Cavia porcellus
3.5.1.1 additional information
-
 additional information
-
 Weizmannia coagulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 additional information
-
-
 Escherichia coli
3.5.1.1 85000
-
 sucrose density gradient centrifugation Weizmannia coagulans
3.5.1.1 128000 145000
-
 Pectobacterium carotovorum
3.5.1.1 133000
-
 gel filtration Cavia porcellus
3.5.1.1 138000
-
 ultracentrifugation Cavia porcellus
3.5.1.1 150000
-
 sucrose density gradient centrifugation Serratia marcescens
3.5.1.1 160000 171000 sucrose density gradient centrifugation Fusarium tricinctum

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Cavia porcellus
-
-
-
3.5.1.1 Erwinia aroidea
-
-
-
3.5.1.1 Escherichia coli
-
-
-
3.5.1.1 Fusarium tricinctum
-
-
-
3.5.1.1 Pectobacterium carotovorum
-
-
-
3.5.1.1 Serratia marcescens
-
-
-
3.5.1.1 Weizmannia coagulans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.1.1 urea denaturation is irreversible Weizmannia coagulans
3.5.1.1 urea denaturation is reversible Escherichia coli
3.5.1.1 urea denaturation is reversible Serratia marcescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.1 serum
-
 Cavia porcellus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 beta-cyano-L-Ala + H2O
-
 Cavia porcellus ?
-
 ?
3.5.1.1 beta-cyano-L-Ala + H2O
-
 Escherichia coli ?
-
 ?
3.5.1.1 beta-cyano-L-Ala + H2O
-
 Pectobacterium carotovorum ?
-
 ?
3.5.1.1 D-Asn + H2O
-
 Escherichia coli D-Asp + NH3
-
 ?
3.5.1.1 D-Asn + H2O
-
 Weizmannia coagulans D-Asp + NH3
-
 ?
3.5.1.1 D-Asn + H2O
-
 Pectobacterium carotovorum D-Asp + NH3
-
 ?
3.5.1.1 D-Asn + H2O 3% of the activity with L-Asn Cavia porcellus D-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Cavia porcellus L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Escherichia coli L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Serratia marcescens L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Weizmannia coagulans L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Pectobacterium carotovorum L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Erwinia aroidea L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Fusarium tricinctum L-Asp + NH3
-
 ?
3.5.1.1 L-Asp + H2O
-
 Escherichia coli ?
-
 ?
3.5.1.1 L-Gln + H2O
-
 Escherichia coli L-Glu + NH3
-
 ?
3.5.1.1 L-Gln + H2O
-
 Pectobacterium carotovorum L-Glu + NH3
-
 ?
3.5.1.1 L-leucine amide + H2O 5.3% of the activity with L-Asn Cavia porcellus ?
-
 ?
3.5.1.1 L-phenylalanine amide + H2O 8.2% of the activity with L-Asn Cavia porcellus ?
-
 ?
3.5.1.1 additional information enzyme has no antitumor activity Weizmannia coagulans ?
-
 ?
3.5.1.1 additional information enzyme has no antitumor activity Fusarium tricinctum ?
-
 ?
3.5.1.1 additional information enzyme has antitumor activity Cavia porcellus ?
-
 ?
3.5.1.1 additional information enzyme has antitumor activity Escherichia coli ?
-
 ?
3.5.1.1 additional information enzyme has antitumor activity Serratia marcescens ?
-
 ?
3.5.1.1 additional information enzyme has antitumor activity Pectobacterium carotovorum ?
-
 ?
3.5.1.1 additional information enzyme has antitumor activity Erwinia aroidea ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 55
-
 10 min, stable Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 6.9
-
 broad Serratia marcescens
3.5.1.1 7.5
-
-
 Erwinia aroidea
3.5.1.1 7.5 8.5
-
 Cavia porcellus
3.5.1.1 7.5 8.7
-
 Fusarium tricinctum
3.5.1.1 8
-
 broad Escherichia coli
3.5.1.1 8.5 9.5
-
 Weizmannia coagulans