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Literature summary extracted from

  • Wriston, J.C.
    Asparaginase (1985), Methods Enzymol., 113, 608-618.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.01
-
 L-asparagine
-
 Pectobacterium carotovorum
3.5.1.1 0.0125
-
 L-asparagine
-
 Escherichia coli
3.5.1.1 0.015
-
 L-asparagine enzyme AG Chlamydomonas sp.
3.5.1.1 0.026
-
 L-asparagine
-
 Proteus vulgaris
3.5.1.1 0.029
-
 L-asparagine
-
 Citrobacter freundii
3.5.1.1 0.0478
-
 L-asparagine
-
 Wolinella succinogenes
3.5.1.1 0.072
-
 L-asparagine
-
 Cavia porcellus
3.5.1.1 0.1
-
 L-asparagine
-
 Serratia marcescens
3.5.1.1 0.11
-
 L-asparagine
-
 Azotobacter vinelandii
3.5.1.1 0.134
-
 L-asparagine
-
 Chlamydomonas sp.
3.5.1.1 0.35
-
 L-asparagine enzyme I Saccharomyces cerevisiae
3.5.1.1 0.5
-
 L-asparagine
-
 Fusarium tricinctum
3.5.1.1 0.74
-
 L-asparagine enzyme I Saccharomyces cerevisiae
3.5.1.1 1
-
 L-asparagine enzyme A Chlamydomonas sp.
3.5.1.1 2
-
 L-asparagine
-
 Acinetobacter calcoaceticus
3.5.1.1 3.2 3.7 L-asparagine
-
 Pisum sativum
3.5.1.1 6.6
-
 L-asparagine
-
 Lupinus arboreus
3.5.1.1 7
-
 L-asparagine
-
 Lupinus angustifolius
3.5.1.1 12.2
-
 L-asparagine
-
 Lupinus polyphyllus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 68300
-
-
 Pisum sativum
3.5.1.1 72000
-
 sucrose density gradient centrifugation Lupinus polyphyllus
3.5.1.1 75000
-
 gel filtration Lupinus angustifolius
3.5.1.1 75000
-
 gel filtration Lupinus arboreus
3.5.1.1 84000
-
-
 Azotobacter vinelandii
3.5.1.1 96000
-
 enzyme A Stenotrophomonas geniculata
3.5.1.1 105000
-
 gel filtration Acinetobacter calcoaceticus
3.5.1.1 120000
-
-
 Proteus vulgaris
3.5.1.1 130000
-
-
 Acinetobacter calcoaceticus
3.5.1.1 130000
-
 ultracentrifugation Escherichia coli
3.5.1.1 135000
-
 ultracentrifugation Pectobacterium carotovorum
3.5.1.1 135000
-
 enzyme AG Stenotrophomonas geniculata
3.5.1.1 138000
-
 ultracentrifugation Cavia porcellus
3.5.1.1 140000
-
-
 Citrobacter freundii
3.5.1.1 141000
-
-
 Klebsiella aerogenes
3.5.1.1 146000
-
-
 Wolinella succinogenes
3.5.1.1 147000
-
 equilibrium sedimentation Serratia marcescens
3.5.1.1 165000
-
 sucrose density gradient centrifugation Fusarium tricinctum
3.5.1.1 275000
-
-
 Chlamydomonas sp.
3.5.1.1 400000
-
 enzyme I Saccharomyces cerevisiae
3.5.1.1 800000
-
 enzyme II Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Acinetobacter calcoaceticus
-
-
-
3.5.1.1 Azotobacter vinelandii
-
-
-
3.5.1.1 Cavia porcellus
-
-
-
3.5.1.1 Chlamydomonas sp.
-
-
-
3.5.1.1 Citrobacter freundii
-
-
-
3.5.1.1 Escherichia coli
-
-
-
3.5.1.1 Fusarium tricinctum
-
-
-
3.5.1.1 Klebsiella aerogenes
-
-
-
3.5.1.1 Lupinus angustifolius
-
-
-
3.5.1.1 Lupinus arboreus
-
-
-
3.5.1.1 Lupinus polyphyllus
-
-
-
3.5.1.1 Pectobacterium carotovorum
-
-
-
3.5.1.1 Pisum sativum
-
-
-
3.5.1.1 Proteus vulgaris
-
-
-
3.5.1.1 Saccharomyces cerevisiae
-
-
-
3.5.1.1 Serratia marcescens
-
-
-
3.5.1.1 Stenotrophomonas geniculata
-
-
-
3.5.1.1 Wolinella succinogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Escherichia coli
3.5.1.1
-
 Wolinella succinogenes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.1 serum
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 1.65
-
-
 Lupinus polyphyllus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 beta-cyano-L-Ala + H2O beta-cyano-L-Ala Cavia porcellus ?
-
 ?
3.5.1.1 beta-cyano-L-Ala + H2O beta-cyano-L-Ala Escherichia coli ?
-
 ?
3.5.1.1 beta-cyano-L-Ala + H2O beta-cyano-L-Ala Pectobacterium carotovorum ?
-
 ?
3.5.1.1 L-Asn + H2O
-
 Cavia porcellus L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Escherichia coli L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Saccharomyces cerevisiae L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Pisum sativum L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Klebsiella aerogenes L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Acinetobacter calcoaceticus L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Serratia marcescens L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Proteus vulgaris L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Azotobacter vinelandii L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Chlamydomonas sp. L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Pectobacterium carotovorum L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Citrobacter freundii L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Lupinus angustifolius L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Lupinus polyphyllus L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Wolinella succinogenes L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Fusarium tricinctum L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Lupinus arboreus L-Asp + NH3
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 6.8
-
 enzyme II Saccharomyces cerevisiae
3.5.1.1 6.9
-
 broad Serratia marcescens
3.5.1.1 6.9 9.5
-
 Chlamydomonas sp.
3.5.1.1 7 8
-
 Proteus vulgaris
3.5.1.1 7 8.2
-
 Klebsiella aerogenes
3.5.1.1 7.5 8.5
-
 Cavia porcellus
3.5.1.1 7.5 8.7
-
 Fusarium tricinctum
3.5.1.1 8
-
-
 Lupinus arboreus
3.5.1.1 8
-
 broad Escherichia coli
3.5.1.1 8.5
-
-
 Serratia marcescens
3.5.1.1 8.5
-
-
 Lupinus angustifolius
3.5.1.1 8.5
-
 enzyme I Saccharomyces cerevisiae
3.5.1.1 8.6
-
-
 Azotobacter vinelandii
3.5.1.1 8.6
-
-
 Pectobacterium carotovorum
3.5.1.1 9
-
 broad, enzyme A Stenotrophomonas geniculata
3.5.1.1 9.5
-
 broad, enzyme AG Stenotrophomonas geniculata