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Literature summary extracted from

  • Yoshimoto, T.; Takahashi, E.; Tsuru, D.
    Purification and enzymatic properties of an endo-alpha-N-acetylgalactosaminidase from Bacillus laterosporus SS-16 (1990), J. Ferment. Bioeng., 70, 348-350.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.97 Ag+ 5 mM, 35% inhibition Brevibacillus laterosporus
3.2.1.97 C2H5HgCl 5 mM, 23% inhibition Brevibacillus laterosporus
3.2.1.97 Ca2+ 5 mM, 26% inhibition Brevibacillus laterosporus
3.2.1.97 Cd2+ 5 mM, 22% inhibition Brevibacillus laterosporus
3.2.1.97 CH3HgCl 5 mM, 18% inhibition Brevibacillus laterosporus
3.2.1.97 Co2+ 5 mM, 18% inhibition Brevibacillus laterosporus
3.2.1.97 Cr3+ 5 mM, 58% inhibition Brevibacillus laterosporus
3.2.1.97 Cu2+ 5 mM, 20% inhibition Brevibacillus laterosporus
3.2.1.97 DFP 1 mM, 48% inhibition Brevibacillus laterosporus
3.2.1.97 Fe3+ 5 mM, 15% inhibition Brevibacillus laterosporus
3.2.1.97 galactonolactone 5 mM, 11% inhibition Brevibacillus laterosporus
3.2.1.97 glucose 5 mM, 11% inhibition Brevibacillus laterosporus
3.2.1.97 Hg2+ 5 mM, 20% inhibition Brevibacillus laterosporus
3.2.1.97 iodoacetate 5 mM, 17% inhibition Brevibacillus laterosporus
3.2.1.97 Mg2+ 5 mM, 17% inhibition Brevibacillus laterosporus
3.2.1.97 Mn2+ 5 mM, 22% inhibition Brevibacillus laterosporus
3.2.1.97 N-bromosuccinimide 1 mM, complete inhibition Brevibacillus laterosporus
3.2.1.97 PCMB 1 mM, 26% inhibition Brevibacillus laterosporus
3.2.1.97 Zn2+ 5 mM, 9% inhibition Brevibacillus laterosporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.97 0.52
-
 asialofetuin
-
 Brevibacillus laterosporus
3.2.1.97 0.78
-
 asialo-kappa-casein
-
 Brevibacillus laterosporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.97 48000
-
 4 * 48000, SDS-PAGE Brevibacillus laterosporus
3.2.1.97 210000
-
 gel filtration Brevibacillus laterosporus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.97 Brevibacillus laterosporus
-
-
-
3.2.1.97 Brevibacillus laterosporus SS-16
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.97
-
 Brevibacillus laterosporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.97 asialo kappa-casein + H2O
-
 Brevibacillus laterosporus Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 asialo kappa-casein + H2O
-
 Brevibacillus laterosporus SS-16 Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 asialo-kappa-casein + H2O
-
 Brevibacillus laterosporus Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 asialo-kappa-casein + H2O
-
 Brevibacillus laterosporus SS-16 Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 asialofetuin + H2O
-
 Brevibacillus laterosporus additional information
-
 ?
3.2.1.97 asialofetuin + H2O
-
 Brevibacillus laterosporus SS-16 additional information
-
 ?
3.2.1.97 asialoglycophorin + H2O
-
 Brevibacillus laterosporus Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 asialoglycophorin + H2O
-
 Brevibacillus laterosporus SS-16 Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 glycoprotein + H2O hydrolyzes the O-glycosidic linkage between alpha-N-acetylgalactosamine and the hydroxyl group of Ser or Thr residues in mucus and mucin-type glycoproteins Brevibacillus laterosporus Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 glycoprotein + H2O hydrolyzes the O-glycosidic linkage between alpha-N-acetylgalactosamine and the hydroxyl group of Ser or Thr residues in mucus and mucin-type glycoproteins Brevibacillus laterosporus SS-16 Galbeta(1-3)GalNAc + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.97 tetramer 4 * 48000, SDS-PAGE Brevibacillus laterosporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.97 35 37
-
 Brevibacillus laterosporus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.97 55
-
 1 h, stable below Brevibacillus laterosporus
3.2.1.97 70
-
 1 h, complete inactivation Brevibacillus laterosporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.97 8
-
-
 Brevibacillus laterosporus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.97 3.5 9 37°C, 1 h, stable Brevibacillus laterosporus