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Literature summary extracted from
- Purification and characterization of 6-phospho-beta-galactosidase from Lactobacillus gasseri JCM 1031 (1996), Biosci. Biotechnol. Biochem., 60, 139-141.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.85 | 2-mercaptoethanol | - |
Lactobacillus gasseri |  |
| 3.2.1.85 | dithiothreitol | - |
Lactobacillus gasseri | |
| 3.2.1.85 | EDTA | 30% increase of activity | Lactobacillus gasseri | |
| 3.2.1.85 | Fe2+ | 60% increase of activity | Lactobacillus gasseri | |
| 3.2.1.85 | glutathione | - |
Lactobacillus gasseri | |
| 3.2.1.85 | L-cysteine | - |
Lactobacillus gasseri | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.85 | iodoacetamide | - |
Lactobacillus gasseri | |
| 3.2.1.85 | N-ethylmaleimide | - |
Lactobacillus gasseri | |
| 3.2.1.85 | p-chloromercuribenzoic acid | - |
Lactobacillus gasseri | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.85 | 0.76 | 0.8 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lactobacillus gasseri | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.85 | Hg2+ | - |
Lactobacillus gasseri | |
| 3.2.1.85 | Zn2+ | - |
Lactobacillus gasseri | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.85 | 58000 | - |
SDS-PAGE | Lactobacillus gasseri |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.85 | lactose-6-phosphate + H2O | Lactobacillus gasseri | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | lactose-6-phosphate + H2O | Lactobacillus gasseri JCM 1031 | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.85 | Lactobacillus gasseri | - |
JCM 1031 | - |
| 3.2.1.85 | Lactobacillus gasseri JCM 1031 | - |
JCM 1031 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.85 | - |
Lactobacillus gasseri |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.85 | lactose-6-phosphate + H2O | - |
Lactobacillus gasseri | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | lactose-6-phosphate + H2O | - |
Lactobacillus gasseri JCM 1031 | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactobacillus gasseri | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactobacillus gasseri JCM 1031 | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.85 | 5 | 5.5 | - |
Lactobacillus gasseri |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.85 | 5 | 7 | at 37°C for 15 min | Lactobacillus gasseri |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.85 | 4 | 9 | at 37°C for 15 min | Lactobacillus gasseri |
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