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Literature summary extracted from
- 6-Phospho-beta-galactosidases of gram-positive and 6-phospho-beta-glucosidase B of gram-negative bacteria: comparison of structure and function by kinetic and immunological methods and mutagenesis of the lacG gene of Staphylococcus aureus (1993), Protein Eng., 6, 913-920.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.85 | expressed in Escherichia coli | Staphylococcus aureus |
| 3.2.1.85 | expressed in Escherichia coli | Lactococcus lactis |
| 3.2.1.85 | expressed in Escherichia coli | Lacticaseibacillus casei |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.85 | E160Q | 1000fold reduction in activity compared to wild type | Staphylococcus aureus |
| 3.2.1.85 | E375Q | complete loss of activity | Staphylococcus aureus |
| 3.2.1.85 | G332R | complete loss of catalytic activity, but same behavior during purification and in affinity to antisera as wild type | Staphylococcus aureus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.85 | D-galactose-6-phosphate | competitive inhibition; weak inhibition | Staphylococcus aureus |  |
| 3.2.1.85 | p-nitrophenyl-beta-thiogalactoside 6-phosphate | competitive inhibition | Staphylococcus aureus | |
| 3.2.1.85 | p-nitrophenyl-beta-thioglucoside 6-phosphate | competitive inhibition | Staphylococcus aureus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.85 | 0.04 | - |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lactococcus lactis | |
| 3.2.1.85 | 0.09 | - |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lactococcus lactis | |
| 3.2.1.85 | 0.1 | - |
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lactococcus lactis | |
| 3.2.1.85 | 0.15 | - |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
| 3.2.1.85 | 0.16 | - |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Staphylococcus aureus | |
| 3.2.1.85 | 0.2 | - |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
| 3.2.1.85 | 0.3 | - |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Staphylococcus aureus | |
| 3.2.1.85 | 0.4 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lactococcus lactis | |
| 3.2.1.85 | 0.4 | - |
p-nitrophenyl-beta-D-glucopyranoside 6 phosphate | - |
Staphylococcus aureus | |
| 3.2.1.85 | 0.6 | - |
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
| 3.2.1.85 | 0.6 | 3 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Staphylococcus aureus | |
| 3.2.1.85 | 1.3 | 1.59 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.85 | lactose-6-phosphate + H2O | Staphylococcus aureus | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | lactose-6-phosphate + H2O | Lactococcus lactis | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | lactose-6-phosphate + H2O | Lacticaseibacillus casei | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.85 | Lacticaseibacillus casei | - |
- |
- |
| 3.2.1.85 | Lactococcus lactis | - |
- |
- |
| 3.2.1.85 | Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.85 | - |
Staphylococcus aureus |
| 3.2.1.85 | - |
Lactococcus lactis |
| 3.2.1.85 | - |
Lacticaseibacillus casei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.85 | lactose-6-phosphate + H2O | - |
Staphylococcus aureus | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | lactose-6-phosphate + H2O | - |
Lactococcus lactis | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | lactose-6-phosphate + H2O | - |
Lacticaseibacillus casei | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Staphylococcus aureus | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactococcus lactis | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lacticaseibacillus casei | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | only little activity | Staphylococcus aureus | o-nitrophenol + beta-D-glucose-6-phosphate | - |
? | |
| 3.2.1.85 | o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | only little activity | Lactococcus lactis | o-nitrophenol + beta-D-glucose-6-phosphate | - |
? | |
| 3.2.1.85 | o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | only little activity | Lacticaseibacillus casei | o-nitrophenol + beta-D-glucose-6-phosphate | - |
? | |
| 3.2.1.85 | p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Staphylococcus aureus | p-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactococcus lactis | p-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lacticaseibacillus casei | p-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
| 3.2.1.85 | p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | only little activity | Staphylococcus aureus | p-nitrophenol + beta-D-glucose-6-phosphate | - |
? | |
| 3.2.1.85 | p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | only little activity | Lactococcus lactis | p-nitrophenol + beta-D-glucose-6-phosphate | - |
? | |
| 3.2.1.85 | p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | only little activity | Lacticaseibacillus casei | p-nitrophenol + beta-D-glucose-6-phosphate | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.85 | 2 | - |
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
| 3.2.1.85 | 4 | - |
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Staphylococcus aureus | |
| 3.2.1.85 | 4 | - |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
| 3.2.1.85 | 5 | - |
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lactococcus lactis | |
| 3.2.1.85 | 7 | - |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lactococcus lactis | |
| 3.2.1.85 | 7 | - |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
| 3.2.1.85 | 9 | - |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Staphylococcus aureus | |
| 3.2.1.85 | 18 | - |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Staphylococcus aureus | |
| 3.2.1.85 | 22 | - |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lactococcus lactis | |
| 3.2.1.85 | 57 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
| 3.2.1.85 | 63 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Staphylococcus aureus | |
| 3.2.1.85 | 83 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lactococcus lactis | |
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