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Literature summary extracted from

  • Witt, E.; Stewart, G.C.; Hengstenberg, W.
    A new protein that replaces the use of secondary antibodies engineered from the two staphylococcal enzymes protein A and 6-phospho-beta-galactosidase (1992), Protein Eng., 5, 267-271.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.85 expressed in Escherichia coli Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.85 additional information hybrid protein with protein A Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.85 D-galactose-6-phosphate
-
 Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.85 0.6 3 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate 2.0 hybrid with protein A Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.85 lactose-6-phosphate + H2O Staphylococcus aureus
-
 D-glucose + beta-D-galactose-6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.85 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.85 hybrid with protein A Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.85 100
-
 maximal activity of hybrid with protein A Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.85 lactose-6-phosphate + H2O
-
 Staphylococcus aureus D-glucose + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
 Staphylococcus aureus o-nitrophenol + beta-D-galactose-6-phosphate
-
 ?