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Literature summary extracted from

  • Calmes, R.; Brown, A.T.
    Regulation of lactose catabolism in Streptococcus mutans: purification and regulatory properties of phospho-beta-galactosidase (1979), Infect. Immun., 23, 68-79.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.85 2-phosphoglycerate 26% inhibition at 4.2 mM Streptococcus mutans
3.2.1.85 ATP 100% inhibition at 2.1 mM Streptococcus mutans
3.2.1.85 D-fructose-1,6-diphosphate 19% inhibition at 4.2 mM Streptococcus mutans
3.2.1.85 D-fructose-6-phosphate 20% inhibition at 4.2 mM Streptococcus mutans
3.2.1.85 D-galactose-6-phosphate 55% inhibition at 4.2 mM Streptococcus mutans
3.2.1.85 D-Glucose-6-phosphate 38% inhibition at 4.2 mM Streptococcus mutans
3.2.1.85 phosphoenolpyruvate 97% inhibition at 1 mM Streptococcus mutans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.85 0.19
-
 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
 Streptococcus mutans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.85 cytoplasm
-
 Streptococcus mutans 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.85 Co2+ 14% inhibition at 5 mM Streptococcus mutans
3.2.1.85 Cu2+ 100% inhibition at 5 mM Streptococcus mutans
3.2.1.85 Zn2+ 57% inhibition at 5 mM Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.85 40000
-
 strain SL1, gel filtration Streptococcus mutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.85 lactose-6-phosphate + H2O Streptococcus mutans
-
 D-glucose + beta-D-galactose-6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.85 Streptococcus mutans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.85
-
 Streptococcus mutans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.85 20.9
-
 purified enzyme Streptococcus mutans

Storage Stability

EC Number Storage Stability Organism
3.2.1.85 -70°C, purified enzyme, at least 6 months Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.85 lactose-6-phosphate + H2O
-
 Streptococcus mutans D-glucose + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
 Streptococcus mutans o-nitrophenol + beta-D-galactose-6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.85 phospho-beta-galactosidase
-
 Streptococcus mutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.85 6.5
-
 most active in Tris-HCl buffer Streptococcus mutans