EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.80 | expression in Escherichia coli | Streptococcus mutans |
3.2.1.154 | fruA is expressed under its own control in Escherichia coli JM83(pFRU1) | Streptococcus mutans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.80 | Ag+ | - |
Streptococcus mutans | |
3.2.1.80 | Cu2+ | partially | Streptococcus mutans | |
3.2.1.80 | Hg2+ | - |
Streptococcus mutans | |
3.2.1.154 | Ag2+ | 1 mM, almost complete inhibition | Streptococcus mutans | |
3.2.1.154 | Cu2+ | 1 mM, about 20% inhibition | Streptococcus mutans | |
3.2.1.154 | Hg2+ | 0.01 mM, almost complete inhibition | Streptococcus mutans | |
3.2.1.154 | Mg2+ | 1 mM, about 20% inhibition | Streptococcus mutans | |
3.2.1.154 | additional information | no inhibition by F- or Tris. No evidence of product inhibition by fructose | Streptococcus mutans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.154 | cytoplasm | - |
Streptococcus mutans | 5737 | - |
3.2.1.154 | cytoplasmic membrane | - |
Streptococcus mutans | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.80 | Co2+ | slight stimulation | Streptococcus mutans | |
3.2.1.80 | Mn2+ | slight stimulation | Streptococcus mutans | |
3.2.1.154 | Co2+ | slight stimulation | Streptococcus mutans | |
3.2.1.154 | Mn2+ | slight stimulation | Streptococcus mutans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.80 | 140000 | - |
x * 140000, SDS-PAGE | Streptococcus mutans |
3.2.1.154 | 140000 | - |
x * 140000, SDS-PAGE | Streptococcus mutans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.80 | fructans + H2O | Streptococcus mutans | degradation | D-fructose | - |
? | |
3.2.1.80 | fructans + H2O | Streptococcus mutans GS-5 | degradation | D-fructose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.80 | Streptococcus mutans | - |
GS-5 | - |
3.2.1.80 | Streptococcus mutans GS-5 | - |
GS-5 | - |
3.2.1.154 | Streptococcus mutans | - |
GS-5 | - |
3.2.1.154 | Streptococcus mutans GS-5 | - |
GS-5 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.80 | - |
Streptococcus mutans |
3.2.1.154 | - |
Streptococcus mutans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.154 | 66.4 | - |
enzyme from Streptococcus mutans | Streptococcus mutans |
3.2.1.154 | 204.5 | - |
recombinant enzyme expressed in Escherichia coli | Streptococcus mutans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.80 | fructans + H2O | degradation | Streptococcus mutans | D-fructose | - |
? | |
3.2.1.80 | fructans + H2O | degradation | Streptococcus mutans GS-5 | D-fructose | - |
? | |
3.2.1.80 | inulin + H2O | - |
Streptococcus mutans | D-fructose + ? | - |
? | |
3.2.1.80 | inulin + H2O | - |
Streptococcus mutans GS-5 | D-fructose + ? | - |
? | |
3.2.1.80 | levan + H2O | - |
Streptococcus mutans | D-fructose + ? | - |
? | |
3.2.1.80 | levan + H2O | - |
Streptococcus mutans GS-5 | D-fructose + ? | - |
? | |
3.2.1.80 | raffinose + H2O | - |
Streptococcus mutans | D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside | - |
? | |
3.2.1.80 | raffinose + H2O | - |
Streptococcus mutans GS-5 | D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside | - |
? | |
3.2.1.80 | sucrose + H2O | - |
Streptococcus mutans | D-fructose + D-glucose | - |
? | |
3.2.1.80 | sucrose + H2O | - |
Streptococcus mutans GS-5 | D-fructose + D-glucose | - |
? | |
3.2.1.154 | inulin + H2O | 34% of the activity with levan | Streptococcus mutans | fructose + ? | - |
? | |
3.2.1.154 | levan + H2O | - |
Streptococcus mutans | fructose + ? | - |
? | |
3.2.1.154 | raffinose + H2O | 12% of the activity with levan | Streptococcus mutans | ? | - |
? | |
3.2.1.154 | sucrose + H2O | 21% of the activity with levan | Streptococcus mutans | D-fructose + D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.80 | ? | x * 140000, SDS-PAGE | Streptococcus mutans |
3.2.1.154 | ? | x * 140000, SDS-PAGE | Streptococcus mutans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.80 | 4.5 | - |
sucrose | Streptococcus mutans |
3.2.1.80 | 5.5 | - |
fructan polymers | Streptococcus mutans |
3.2.1.154 | 4.5 | - |
sucrose degradation | Streptococcus mutans |
3.2.1.154 | 5.5 | - |
reaction with levan or inulin | Streptococcus mutans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.80 | 3.5 | 7 | little fructanase activity at or above pH 7.0 | Streptococcus mutans |
3.2.1.154 | 4 | 6 | pH 4.0: about 85% of maximal activity, pH 6.0: about 70% of maximal activity, reaction with sucrose | Streptococcus mutans |
3.2.1.154 | 4 | 7 | pH 4.0: about 40% of maximal activity, pH 7.0: about 50% of maximal activity, reaction with levan or inulin | Streptococcus mutans |