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Literature summary extracted from

  • Ahmed, S.A.; Esaki, N.; Tanaka, H.; Soda, K.
    Properties of alpha-amino-epsilon-caprolactam racemase from Achromobacter obae (1983), Agric. Biol. Chem., 47, 1887-1893.
No PubMed abstract available

General Stability

EC Number General Stability Organism
5.1.1.15 freezing inactivates, particularly in dilute solution of less than 1 mg/ml Achromobacter obae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.15 2-Methyl-3-benzothiazolonehydrazone hydrochloride
-
 Achromobacter obae
5.1.1.15 Ala weak Achromobacter obae
5.1.1.15 CuSO4
-
 Achromobacter obae
5.1.1.15 D-cycloserine
-
 Achromobacter obae
5.1.1.15 epsilon-Caprolactam
-
 Achromobacter obae
5.1.1.15 Glu weak Achromobacter obae
5.1.1.15 Gly
-
 Achromobacter obae
5.1.1.15 HgCl2
-
 Achromobacter obae
5.1.1.15 hydroxylamine
-
 Achromobacter obae
5.1.1.15 L-His
-
 Achromobacter obae
5.1.1.15 L-Lys
-
 Achromobacter obae
5.1.1.15 L-Met
-
 Achromobacter obae
5.1.1.15 L-Orn
-
 Achromobacter obae
5.1.1.15 Leu weak Achromobacter obae
5.1.1.15 NEM
-
 Achromobacter obae
5.1.1.15 PCMB
-
 Achromobacter obae
5.1.1.15 phenylhydrazine
-
 Achromobacter obae
5.1.1.15 Pro weak Achromobacter obae
5.1.1.15 Sodium borohydride
-
 Achromobacter obae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.15 6
-
 L-alpha-amino-epsilon-caprolactam
-
 Achromobacter obae
5.1.1.15 8
-
 D-alpha-Amino-epsilon-caprolactam
-
 Achromobacter obae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.1.15 cytoplasm
-
 Achromobacter obae 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.15 49000
-
 1 * 49000, SDS-PAGE Achromobacter obae
5.1.1.15 51000
-
 gel filtration Achromobacter obae

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.15 Achromobacter obae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.15
-
 Achromobacter obae

Storage Stability

EC Number Storage Stability Organism
5.1.1.15 -20°C, 0.25 M sucrose, 10 mM potassium phosphate buffer, pH 7.3, 0.02 mM pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, protein concentration: more than 0.3%, stable for at least 6 months Achromobacter obae
5.1.1.15 20°C, 0.25 M sucrose, enzyme retains approximately 90% of its initial activity after storage for 4 months Achromobacter obae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.15 L-alpha-Amino-epsilon-caprolactam r Achromobacter obae D-alpha-Amino-epsilon-caprolactam
-
 ?
5.1.1.15 additional information the enzyme catalyzes alpha-proton exchange of the substrate with deuterium during racemization in deuterium oxide Achromobacter obae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.15 monomer 1 * 49000, SDS-PAGE Achromobacter obae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.15 60
-
 pH 7.2, 20 min, stable Achromobacter obae
5.1.1.15 65
-
 pH 7.2, 20 min, 5% loss of activity Achromobacter obae
5.1.1.15 70
-
 pH 7.2, 20 min, 15% loss of activity Achromobacter obae
5.1.1.15 75
-
 pH 7.2, 20 min, 40% loss of activity Achromobacter obae
5.1.1.15 80
-
 pH 7.2, 20 min, 25% loss of activity Achromobacter obae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.15 8.8
-
 L-alpha-amino-epsilon-caprolactam Achromobacter obae
5.1.1.15 8.8
-
 D-alpha-amino-epsilon-caprolactam Achromobacter obae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.1.15 6 9 at 25°C, 20 min, stable Achromobacter obae

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.15 pyridoxal 5'-phosphate enzyme contains 1 mol of pyridoxal 5'-phosphate per mol of enzyme. Km: 0.00021 mM Achromobacter obae