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Literature summary extracted from

  • McCleary, B.V.
    beta-D-Mannanase (1988), Methods Enzymol., 160, 596-610.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 additional information
-
 additional information
-
 Bacillus subtilis
3.2.1.78 additional information
-
 additional information
-
 Aspergillus niger
3.2.1.78 additional information
-
 additional information
-
 Medicago sativa
3.2.1.78 additional information
-
 additional information
-
 Helix pomatia
3.2.1.78 additional information
-
 additional information
-
 Cyamopsis tetragonoloba
3.2.1.78 additional information
-
 additional information
-
 Irpex lacteus
3.2.1.78 1.9
-
 soluble mannan
-
 Medicago sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 37000
-
 SDS-PAGE Bacillus subtilis
3.2.1.78 37000
-
 SDS-PAGE Helix pomatia
3.2.1.78 41000
-
 SDS-PAGE Medicago sativa
3.2.1.78 45000
-
-
 Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Aspergillus niger
-
 commercial preparations
-
3.2.1.78 Bacillus subtilis
-
 commercial preparation
-
3.2.1.78 Cyamopsis tetragonoloba
-
 guar
-
3.2.1.78 Helix pomatia
-
 snail
-
3.2.1.78 Irpex lacteus
-
-
-
3.2.1.78 Medicago sativa
-
 lucerne
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78
-
 Bacillus subtilis
3.2.1.78
-
 Medicago sativa
3.2.1.78
-
 Helix pomatia
3.2.1.78
-
 Cyamopsis tetragonoloba
3.2.1.78
-
 Irpex lacteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.78 commercial preparation
-
 Bacillus subtilis
-
3.2.1.78 commercial preparation
-
 Aspergillus niger
-
3.2.1.78 commercial preparation
-
 Medicago sativa
-
3.2.1.78 commercial preparation
-
 Helix pomatia
-
3.2.1.78 culture filtrate
-
 Bacillus subtilis
-
3.2.1.78 culture filtrate
-
 Medicago sativa
-
3.2.1.78 culture filtrate
-
 Irpex lacteus
-
3.2.1.78 endosperm
-
 Bacillus subtilis
-
3.2.1.78 endosperm
-
 Aspergillus niger
-
3.2.1.78 endosperm
-
 Medicago sativa
-
3.2.1.78 endosperm
-
 Helix pomatia
-
3.2.1.78 endosperm
-
 Cyamopsis tetragonoloba
-
3.2.1.78 endosperm
-
 Irpex lacteus
-
3.2.1.78 gut
-
 Bacillus subtilis
-
3.2.1.78 gut
-
 Aspergillus niger
-
3.2.1.78 gut
-
 Medicago sativa
-
3.2.1.78 gut
-
 Helix pomatia
-
3.2.1.78 seed
-
 Medicago sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 additional information
-
-
 Bacillus subtilis
3.2.1.78 additional information
-
-
 Aspergillus niger
3.2.1.78 additional information
-
-
 Medicago sativa
3.2.1.78 additional information
-
-
 Helix pomatia
3.2.1.78 additional information
-
-
 Cyamopsis tetragonoloba
3.2.1.78 additional information
-
-
 Irpex lacteus
3.2.1.78 39
-
-
 Aspergillus niger
3.2.1.78 125
-
-
 Cyamopsis tetragonoloba
3.2.1.78 514
-
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
3.2.1.78 4°C, stable for 18 h, pH 4-10 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 galactoglucomannan + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.78 galactoglucomannan + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.78 galactoglucomannan + H2O
-
 Medicago sativa ?
-
 ?
3.2.1.78 galactoglucomannan + H2O
-
 Helix pomatia ?
-
 ?
3.2.1.78 galactoglucomannan + H2O
-
 Cyamopsis tetragonoloba ?
-
 ?
3.2.1.78 galactoglucomannan + H2O
-
 Irpex lacteus ?
-
 ?
3.2.1.78 galactomannan + H2O
-
 Bacillus subtilis hydrolyzed galactomannan
-
 ?
3.2.1.78 galactomannan + H2O
-
 Aspergillus niger hydrolyzed galactomannan
-
 ?
3.2.1.78 galactomannan + H2O
-
 Medicago sativa hydrolyzed galactomannan
-
 ?
3.2.1.78 galactomannan + H2O
-
 Helix pomatia hydrolyzed galactomannan
-
 ?
3.2.1.78 glucomannan + H2O
-
 Bacillus subtilis oligosaccharides
-
 ?
3.2.1.78 glucomannan + H2O
-
 Aspergillus niger oligosaccharides
-
 ?
3.2.1.78 glucomannan + H2O
-
 Medicago sativa oligosaccharides
-
 ?
3.2.1.78 glucomannan + H2O
-
 Helix pomatia oligosaccharides
-
 ?
3.2.1.78 glucomannan + H2O
-
 Cyamopsis tetragonoloba oligosaccharides
-
 ?
3.2.1.78 glucomannan + H2O
-
 Irpex lacteus oligosaccharides
-
 ?
3.2.1.78 mannan + H2O
-
 Bacillus subtilis oligosaccharides
-
 ?
3.2.1.78 mannan + H2O
-
 Aspergillus niger oligosaccharides
-
 ?
3.2.1.78 mannan + H2O
-
 Medicago sativa oligosaccharides
-
 ?
3.2.1.78 mannan + H2O
-
 Helix pomatia oligosaccharides
-
 ?
3.2.1.78 mannan + H2O
-
 Cyamopsis tetragonoloba oligosaccharides
-
 ?
3.2.1.78 mannan + H2O
-
 Irpex lacteus oligosaccharides
-
 ?
3.2.1.78 mannopentaose + H2O
-
 Bacillus subtilis mannose + mannobiose + mannotriose + mannotetraose
-
 ?
3.2.1.78 mannopentaose + H2O
-
 Aspergillus niger mannose + mannobiose + mannotriose + mannotetraose
-
 ?
3.2.1.78 mannopentaose + H2O
-
 Medicago sativa mannose + mannobiose + mannotriose + mannotetraose
-
 ?
3.2.1.78 mannopentaose + H2O
-
 Helix pomatia mannose + mannobiose + mannotriose + mannotetraose
-
 ?
3.2.1.78 mannopentaose + H2O
-
 Cyamopsis tetragonoloba mannose + mannobiose + mannotriose + mannotetraose
-
 ?
3.2.1.78 mannopentaose + H2O
-
 Irpex lacteus mannose + mannobiose + mannotriose + mannotetraose
-
 ?
3.2.1.78 mannotetraose + H2O
-
 Bacillus subtilis mannobiose + mannose + mannotriose
-
 ?
3.2.1.78 mannotetraose + H2O
-
 Medicago sativa mannobiose + mannose + mannotriose
-
 ?
3.2.1.78 mannotetraose + H2O
-
 Helix pomatia mannobiose + mannose + mannotriose
-
 ?
3.2.1.78 mannotetraose + H2O
-
 Aspergillus niger mannobiose + mannose + mannotriose small amounts of mannose and mannotriose ?
3.2.1.78 mannotriose + H2O
-
 Aspergillus niger mannobiose + mannose
-
 ?
3.2.1.78 additional information not: mannobiose Aspergillus niger ?
-
 ?
3.2.1.78 soluble mannan + H2O
-
 Medicago sativa mannosyl oligosaccharides
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 additional information
-
-
 Bacillus subtilis
3.2.1.78 additional information
-
-
 Aspergillus niger
3.2.1.78 additional information
-
-
 Medicago sativa
3.2.1.78 additional information
-
-
 Helix pomatia
3.2.1.78 additional information
-
-
 Cyamopsis tetragonoloba
3.2.1.78 additional information
-
-
 Irpex lacteus
3.2.1.78 80
-
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.78 additional information
-
-
 Bacillus subtilis
3.2.1.78 additional information
-
-
 Aspergillus niger
3.2.1.78 additional information
-
-
 Medicago sativa
3.2.1.78 additional information
-
-
 Helix pomatia
3.2.1.78 additional information
-
-
 Cyamopsis tetragonoloba
3.2.1.78 additional information
-
-
 Irpex lacteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 additional information
-
-
 Bacillus subtilis
3.2.1.78 additional information
-
-
 Aspergillus niger
3.2.1.78 additional information
-
-
 Medicago sativa
3.2.1.78 additional information
-
-
 Helix pomatia
3.2.1.78 additional information
-
-
 Cyamopsis tetragonoloba
3.2.1.78 additional information
-
-
 Irpex lacteus
3.2.1.78 39
-
 4 h, stable between pH 5 and 7 Aspergillus niger
3.2.1.78 50
-
 stable below Bacillus subtilis
3.2.1.78 50
-
 stable below Medicago sativa
3.2.1.78 50
-
 stable below Helix pomatia
3.2.1.78 70
-
-
 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 additional information
-
-
 Bacillus subtilis
3.2.1.78 additional information
-
-
 Aspergillus niger
3.2.1.78 additional information
-
-
 Medicago sativa
3.2.1.78 additional information
-
-
 Helix pomatia
3.2.1.78 additional information
-
-
 Cyamopsis tetragonoloba
3.2.1.78 additional information
-
-
 Irpex lacteus
3.2.1.78 3
-
-
 Aspergillus niger
3.2.1.78 3.6
-
-
 Aspergillus niger
3.2.1.78 4
-
-
 Cyamopsis tetragonoloba
3.2.1.78 4.5 5.5
-
 Helix pomatia
3.2.1.78 5 6
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 additional information
-
-
 Bacillus subtilis
3.2.1.78 additional information
-
-
 Aspergillus niger
3.2.1.78 additional information
-
-
 Medicago sativa
3.2.1.78 additional information
-
-
 Helix pomatia
3.2.1.78 additional information
-
-
 Cyamopsis tetragonoloba
3.2.1.78 additional information
-
-
 Irpex lacteus
3.2.1.78 3 8
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 additional information
-
-
 Bacillus subtilis
3.2.1.78 additional information
-
-
 Aspergillus niger
3.2.1.78 additional information
-
-
 Medicago sativa
3.2.1.78 additional information
-
-
 Helix pomatia
3.2.1.78 additional information
-
-
 Cyamopsis tetragonoloba
3.2.1.78 additional information
-
-
 Irpex lacteus
3.2.1.78 4 8 40°C, 1 h Medicago sativa
3.2.1.78 5 8
-
 Helix pomatia