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Literature summary extracted from
- Enzymatic properties of rat lactase-phlorizin hydrolase (1974), Biochim. Biophys. Acta, 350, 100-112.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.7.1.4 | 2-mercaptoethanol | - |
Rattus norvegicus |  |
| 3.7.1.4 | cysteine | - |
Rattus norvegicus | |
| 3.7.1.4 | dithiothreitol | - |
Rattus norvegicus | |
| 3.7.1.4 | HgCl2 | - |
Rattus norvegicus | |
| 3.7.1.4 | iodoacetate | - |
Rattus norvegicus | |
| 3.7.1.4 | N-ethylmaleimide | - |
Rattus norvegicus | |
| 3.7.1.4 | p-chloromercuribenzoate | - |
Rattus norvegicus | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.62 | at neutral pH and 37°C, lactase acitivity rapidly lost, partially prevented by p-chloromercuribenzoate, more stable if attached to its border-membrane | Rattus sp. |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.62 | 41.7 | - |
4-O-beta-D-galactopyranosyl-D-glucopyranoside | - |
Rattus sp. | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.62 | brush border | - |
Rattus sp. | 5903 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.62 | 3'-fucosyllactose + H2O | Rattus sp. | only oligosaccharides containing galactose as the glycone in beta-linkage hydrolysed | beta-D-glucose + 3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-galactopyranose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | Rattus sp. | two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis | D-galactose + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Rattus sp. | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.62 | lacto-N-tetraose + H2O | Rattus sp. | - |
beta-D-glucose + ? | - |
? | |
| 3.2.1.62 | phlorizin + H2O | Rattus sp. | only substrate that is hydrolysed at the active site for phlorizin hydrolysis | phloretin + beta-D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.62 | Rattus sp. | - |
- |
- |
| 3.7.1.4 | Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.62 | glycoprotein | 17% carbohydrate content, consisting of galactose, mannose, fucose, N-acetylglucosamine | Rattus sp. |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.62 | partial | Rattus sp. |
| 3.7.1.4 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.62 | intestine | - |
Rattus sp. | - |
| 3.7.1.4 | intestinal mucosa | - |
Rattus norvegicus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.62 | -20°C, several months | Rattus sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.62 | 3'-fucosyllactose + H2O | only oligosaccharides containing galactose as the glycone in beta-linkage hydrolysed | Rattus sp. | beta-D-glucose + 3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-galactopyranose | - |
? | |
| 3.2.1.62 | 4-methylumbelliferyl-beta-D-galactoside + H2O | - |
Rattus sp. | 4-methylumbelliferone + beta-D-galactose | - |
? | |
| 3.2.1.62 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Rattus sp. | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis | Rattus sp. | D-galactose + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis | Rattus sp. | D-galactose + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Rattus sp. | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.62 | lacto-N-tetraose + H2O | - |
Rattus sp. | beta-D-glucose + ? | - |
? | |
| 3.2.1.62 | phlorizin + H2O | only substrate that is hydrolysed at the active site for phlorizin hydrolysis | Rattus sp. | phloretin + beta-D-glucose | - |
? | |
| 3.7.1.4 | lactose + H2O | - |
Rattus norvegicus | D-galactose + D-glucose | - |
? | |
| 3.7.1.4 | phlorhizin + H2O | - |
Rattus norvegicus | 3-(4-hydroxyphenyl)propanoate + arbutin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.7.1.4 | pentamer | SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.4 | -20 | - |
stable for months | Rattus norvegicus |
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