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Literature summary extracted from
- The glycosylceramidase in the murine intestine. Purification and substrate specificity (1981), J. Biol. Chem., 256, 7768-7773.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.62 | taurodeoxycholate | activation | Mus musculus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.62 | 0.01 | - |
glucosylceramide | - |
Mus musculus | |
| 3.2.1.62 | 0.065 | - |
galactosylceramide | - |
Mus musculus | |
| 3.2.1.62 | 0.18 | - |
phlorizin | - |
Mus musculus | |
| 3.2.1.62 | 0.24 | - |
galactosylsphingosine | - |
Mus musculus | |
| 3.2.1.62 | 0.24 | - |
4-methylumbelliferyl-beta-D-galactoside | - |
Mus musculus | |
| 3.2.1.62 | 0.27 | - |
lactosylceramide | - |
Mus musculus | |
| 3.2.1.62 | 0.36 | - |
4-nitrophenyl-beta-D-fucoside | - |
Mus musculus | |
| 3.2.1.62 | 0.37 | - |
glucosylsphingosine | - |
Mus musculus | |
| 3.2.1.62 | 0.45 | - |
4-methylumbelliferyl-alpha-L-arabinoside | - |
Mus musculus | |
| 3.2.1.62 | 0.53 | - |
4-methylumbelliferyl-beta-D-glucoside | - |
Mus musculus | |
| 3.2.1.62 | 1.3 | - |
4-O-beta-D-glucopyranosyl-D-glucopyranoside | - |
Mus musculus | |
| 3.2.1.62 | 1.4 | - |
4-methylumbelliferyl-beta-D-xyloside | - |
Mus musculus | |
| 3.2.1.62 | 2.78 | - |
4-O-beta-D-glucopyranosyl-D-glucopyranoside | - |
Mus musculus | |
| 3.2.1.62 | 4.8 | - |
4-O-beta-D-galactopyranosyl-D-glucosamine | - |
Mus musculus | |
| 3.2.1.62 | 22 | - |
6-O-beta-D-glucopyranosyl-D-glucopyranoside | - |
Mus musculus | |
| 3.2.1.62 | 28 | - |
4-O-beta-D-galactopyranosyl-D-glucopyranoside | - |
Mus musculus | |
| 3.2.1.62 | 67 | - |
4-O-beta-D-galactopyranosyl-D-fructoside | - |
Mus musculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.62 | 290000 | 300000 | gel filtration | Mus musculus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-fructoside + H2O | Mus musculus | very low activity | beta-D-galactose + D-fructose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | Mus musculus | best substrate | D-galactose + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-N-acetylglucosamine + H2O | Mus musculus | poor activity | beta-D-galactose + N-acetylglucosamine | - |
? | |
| 3.2.1.62 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Mus musculus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.62 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Mus musculus | low activity | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.62 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Mus musculus | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.62 | galactosylceramide + H2O | Mus musculus | - |
beta-D-galactose + ceramide | - |
ir | |
| 3.2.1.62 | galactosylsphingosine + H2O | Mus musculus | very low activity | beta-D-galactose + sphingosine | - |
? | |
| 3.2.1.62 | glucosylceramide + H2O | Mus musculus | - |
beta-D-glucose + ceramide | - |
? | |
| 3.2.1.62 | glucosylsphingosine + H2O | Mus musculus | very low activity | beta-D-glucose + N-acylsphingosine | - |
? | |
| 3.2.1.62 | lactosylceramide + H2O | Mus musculus | low activity | beta-D-glucose + galactosylceramide | - |
? | |
| 3.2.1.62 | phlorizin + H2O | Mus musculus | low activity | phloretin + beta-D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.62 | Mus musculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.62 | to homogeneity, chromatography steps | Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.62 | intestine | small intestine | Mus musculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.62 | 1.78 | - |
galactosylceramide hydrolysis | Mus musculus |
| 3.2.1.62 | 2.67 | - |
glucosylceramide hydrolysis | Mus musculus |
| 3.2.1.62 | 15.33 | - |
lactose hydrolysis | Mus musculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.62 | 4-methylumbelliferyl-alpha-L-arabinoside + H2O | - |
Mus musculus | 4-methylumbelliferone + alpha-L-arabinose | - |
? | |
| 3.2.1.62 | 4-methylumbelliferyl-beta-D-galactoside + H2O | - |
Mus musculus | 4-methylumbelliferone + beta-D-galactose | - |
? | |
| 3.2.1.62 | 4-methylumbelliferyl-beta-D-glucoside + H2O | - |
Mus musculus | 4-methylumbelliferone + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-methylumbelliferyl-beta-D-xyloside + H2O | low activity | Mus musculus | 4-methylumbelliferone + beta-D-xylose | - |
? | |
| 3.2.1.62 | 4-nitrophenyl beta-D-fucoside + H2O | - |
Mus musculus | 4-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-fructoside + H2O | very low activity | Mus musculus | beta-D-galactose + D-fructose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | best substrate | Mus musculus | D-galactose + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-glucosamine + H2O | - |
Mus musculus | ? | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-N-acetylglucosamine + H2O | poor activity | Mus musculus | beta-D-galactose + N-acetylglucosamine | - |
? | |
| 3.2.1.62 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Mus musculus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.62 | 6-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O | - |
Mus musculus | ? | - |
? | |
| 3.2.1.62 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Mus musculus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.62 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Mus musculus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.62 | galactosylceramide + H2O | - |
Mus musculus | beta-D-galactose + ceramide | - |
? | |
| 3.2.1.62 | galactosylceramide + H2O | - |
Mus musculus | beta-D-galactose + ceramide | - |
ir | |
| 3.2.1.62 | galactosylsphingosine + H2O | very low activity | Mus musculus | beta-D-galactose + sphingosine | - |
? | |
| 3.2.1.62 | glucosylceramide + H2O | - |
Mus musculus | beta-D-glucose + ceramide | - |
? | |
| 3.2.1.62 | glucosylsphingosine + H2O | very low activity | Mus musculus | beta-D-glucose + N-acylsphingosine | - |
? | |
| 3.2.1.62 | lactosylceramide + H2O | low activity | Mus musculus | beta-D-glucose + galactosylceramide | - |
? | |
| 3.2.1.62 | phlorizin + H2O | low activity | Mus musculus | phloretin + beta-D-glucose | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.62 | 45 | - |
more than 90% of activity lost within 20 min | Mus musculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.62 | 5.2 | - |
hydrolysis of galactosyl- or glucosylceramide in the presence of taurodeoxycholate | Mus musculus |
| 3.2.1.62 | 5.5 | 5.7 | hydrolysis of disaccharides or artificial substrates | Mus musculus |
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