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Literature summary extracted from

  • Ogata-Arakawa, M.; Muramatsu, T.; Kobata, A.
    alpha-L-Fucosidases from almond emulsin: characterization of the two enzymes with different specificities (1977), Arch. Biochem. Biophys., 181, 353-358.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.111 NaCl 0.1 M, required for optimal activity Prunus dulcis

General Stability

EC Number General Stability Organism
3.2.1.111 major loss of activity by ion-exchange chromatography Prunus dulcis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 4-chloromercuriphenylsulfonic acid slight inhibition at 1 mM Prunus dulcis
3.2.1.51 Ca2+ alpha-fucosidase I, slight inhibition at 10 mM Prunus dulcis
3.2.1.51 L-cysteine alpha-fucosidase I, slight inhibition at 10 mM Prunus dulcis
3.2.1.51 Mg2+ slight inhibition at 10 mM Prunus dulcis
3.2.1.63 MgCl2
-
 Prunus dulcis
3.2.1.63 p-chloromercuriphenylsulfonate 1 mM, 12% inhibition Prunus dulcis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.095
-
 lacto-N-fucopentaitol III alpha-fucosidase I Prunus dulcis
3.2.1.51 0.1
-
 lacto-N-fucopentaitol II alpha-fucosidase I Prunus dulcis
3.2.1.51 0.67
-
 2'-fucosyllactitol alpha-fucosidase II Prunus dulcis
3.2.1.63 0.675
-
 2-fucosyllactitol
-
 Prunus dulcis
3.2.1.111 0.095
-
 lacto-N-fucopentaitol III
-
 Prunus dulcis
3.2.1.111 0.101
-
 lacto-N-fucopentaitol II pH 5.0, 37°C Prunus dulcis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.51 Ca2+ alpha-fucosidase II, 21% activation at 10 mM Prunus dulcis
3.2.1.63 CaCl2 slight activation Prunus dulcis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.51 2'-fucosyllactitol + H2O Prunus dulcis alpha-fucosidase II highly active alpha-L-fucose + lactitol
-
 ?
3.2.1.51 3-fucosyllactitol + H2O Prunus dulcis low activity alpha-L-fucose + lactitol
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O Prunus dulcis only hydrolysed by alpha-L-fucosidase II Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O Prunus dulcis lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O Prunus dulcis only hydrolysed by alpha-fucosidase II alpha-L-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O Prunus dulcis 2'-fucosyllactose alpha-L-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O Prunus dulcis low activity alpha-L-fucose + Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc
-
 ?
3.2.1.51 Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O Prunus dulcis low activity for alpha-L-fucosidase II alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O Prunus dulcis lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 lacto-N-fucopentaitol III + H2O Prunus dulcis low activity for alpha-L-fucosidase II alpha-L-fucose + ?
-
 ?
3.2.1.111 additional information Prunus dulcis the enzyme hydrolyzes fucose alpha-1,3 or alpha-1,4-linkages to N-acteylglucosamine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Prunus dulcis
-
 almond
-
3.2.1.63 Prunus dulcis
-
 almond
-
3.2.1.111 Prunus dulcis
-
 almond
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 partial, two forms Prunus dulcis
3.2.1.63 partial Prunus dulcis
3.2.1.111 partial Prunus dulcis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 emulsin two forms, alpha-fucosidase I and II Prunus dulcis
-
3.2.1.63 emulsin
-
 Prunus dulcis
-
3.2.1.111 emulsin
-
 Prunus dulcis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 0.19
-
 alpha-L-fucosidase II Prunus dulcis
3.2.1.51 1.46
-
 alpha-L-fucosidase I Prunus dulcis
3.2.1.63 0.00186
-
-
 Prunus dulcis
3.2.1.111 0.00146
-
-
 Prunus dulcis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 2'-fucosyllactitol + H2O alpha-fucosidase II highly active Prunus dulcis alpha-L-fucose + lactitol
-
 ?
3.2.1.51 3-fucosyllactitol + H2O low activity Prunus dulcis alpha-L-fucose + lactitol
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O only hydrolysed by alpha-L-fucosidase II Prunus dulcis Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide Prunus dulcis Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O only hydrolysed by alpha-fucosidase II Prunus dulcis alpha-L-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O 2'-fucosyllactose Prunus dulcis alpha-L-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O low activity Prunus dulcis alpha-L-fucose + Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc
-
 ?
3.2.1.51 Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O low activity for alpha-L-fucosidase II Prunus dulcis alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide Prunus dulcis alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide Prunus dulcis alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 lacto-N-fucopentaitol II + H2O
-
 Prunus dulcis alpha-L-fucose + ?
-
 ?
3.2.1.51 lacto-N-fucopentaitol III + H2O low activity for alpha-L-fucosidase II Prunus dulcis alpha-L-fucose + ?
-
 ?
3.2.1.63 2'-fucosyllactose + H2O
-
 Prunus dulcis fucose + lactose
-
 ?
3.2.1.63 2-fucosyllactitol + H2O
-
 Prunus dulcis fucose + lactitol
-
 ?
3.2.1.63 2-O-alpha-L-fucopyranosyl-D-galactose + H2O
-
 Prunus dulcis L-fucose + galactose
-
 ?
3.2.1.63 additional information highly specific for non-reducing terminal L-fucose residues linked to D-galactose residues by 1,2-alpha linkage Prunus dulcis ?
-
 ?
3.2.1.111 lacto-N-difucohexaitol + H2O poor substrate Prunus dulcis ?
-
 ?
3.2.1.111 lacto-N-fucopentaitol + H2O lacto-N-fucopentaitol II or III, no substrate: lacto-N-fucopentaitol I Prunus dulcis ?
-
 ?
3.2.1.111 lacto-N-fucopentaitol II + H2O
-
 Prunus dulcis ?
-
 ?
3.2.1.111 lacto-N-fucopentaitol III + H2O
-
 Prunus dulcis ?
-
 ?
3.2.1.111 additional information no hydrolysis of fucose alpha-1,6 or alpha-1,2-linkages to N-acetylglucosamine, as in IgG glycopeptides or 2'-fucosyllactitol Prunus dulcis ?
-
 ?
3.2.1.111 additional information the enzyme hydrolyzes fucose alpha-1,3 or alpha-1,4-linkages to N-acteylglucosamine Prunus dulcis ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 5 6.5 alpha-fucosidase II Prunus dulcis
3.2.1.51 5.5
-
 alpha-fucosidase I Prunus dulcis
3.2.1.63 5 6.5
-
 Prunus dulcis
3.2.1.111 5.5
-
-
 Prunus dulcis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.111 4 6.8 about half-maximal activity at pH 4 and 6.8 Prunus dulcis