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Literature summary extracted from

  • Opheim, D.J.; Touster, O.
    alpha-L-Fucosidase from rat liver lysosomes (1978), Methods Enzymol., 50, 505-510.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.51 stabilizing effect by addition of 0.05 mM/ml bovine serum albumin, 1 mM 2-mercaptoethanol, 1 mM MgSO4, high protein concentrations required Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 4-chloromercuriphenylsulfonic acid complete inhibition at 0.002 mM Rattus sp.
3.2.1.51 alpha-L-fucose competitive inhibition, Ki: 1.6 mM Rattus sp.
3.2.1.51 Cu2+
-
 Rattus sp.
3.2.1.51 Hg2+
-
 Rattus sp.
3.2.1.51 Ni2+
-
 Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.19
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.51 lysosome
-
 Rattus sp. 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 55000
-
 4 * 55000, SDS-PAGE Rattus sp.
3.2.1.51 217000
-
 sedimentation equilibrium centrifugation Rattus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Rattus sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.51 glycoprotein residues per tetramer: fucose 3.5, mannose 32, galactose 8: glucose 9, glucosamine 32, sialic acid 8 Rattus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 to homogeneity, affinity chromatography Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 27 28
-
 Rattus sp.

Storage Stability

EC Number Storage Stability Organism
3.2.1.51 -20°C, acetate buffer, pH 6.0, 100 mM L-fucose, several months Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages Rattus sp. 4-nitrophenol + alpha-L-fucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 tetramer 4 * 55000, SDS-PAGE Rattus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 25
-
 50% remaining activity after 2 h Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 5.5 5.8
-
 Rattus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.51 4 8 stable in the presence of 0.05 mg/mg bovine serum albumin, 1 mM 2-mercaptoethanol Rattus sp.