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Literature summary extracted from

  • Ajisaka, K.; Fujimoto, H.; Miyasato, M.
    An alpha-L-fucosidase from Penicillium multicolor as a candidate enzyme for the synthesis of alpha (1-3)-linked fucosyl oligosaccharides by transglycosylation (1998), Carbohydr. Res., 309, 125-129.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.51 extracellular
-
 Aspergillus niger
-
-
3.2.1.51 extracellular
-
 Penicillium multicolor
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 180000
-
 gel filtration Penicillium multicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.51 alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine Penicillium multicolor same yield of transglycosylation and hydrolysis products alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
 ?
3.2.1.51 alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine Penicillium multicolor slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
 ?
3.2.1.51 alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine Penicillium multicolor same yield of transglycosylation and hydrolysis products alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
-
 ?
3.2.1.51 Fuc-alpha-(1-3)-GlcNAc + H2O Aspergillus niger slow hydrolysis rate alpha-L-fucose + N-acetyl-D-glucosamine
-
 ?
3.2.1.51 Fuc-alpha-(1-3)-GlcNAc + H2O Penicillium multicolor slow hydrolysis rate alpha-L-fucose + N-acetyl-D-glucosamine
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.51 dimethyl formamide
-
 Aspergillus niger
3.2.1.51 dimethyl formamide more stable than the Aspergillus niger enzyme, no loss of activity up to 10%, v/v Penicillium multicolor
3.2.1.51 dimethyl sulfoxide
-
 Aspergillus niger
3.2.1.51 dimethyl sulfoxide more stable than the Aspergillus niger enzyme, no loss of activity up to 20%, v/v Penicillium multicolor
3.2.1.51 dioxane
-
 Aspergillus niger
3.2.1.51 dioxane more stable than the Aspergillus niger enzyme, no loss of activity up to 10%, v/v Penicillium multicolor

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Aspergillus niger
-
-
-
3.2.1.51 Penicillium multicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 partial Penicillium multicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 commercial preparation lactase-P Penicillium multicolor
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 0.04
-
-
 Penicillium multicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O transglycosylation activity Aspergillus niger 4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc no other isomer ?
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O transglycosylation activity Penicillium multicolor 4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc no other isomer ?
3.2.1.51 alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine same yield of transglycosylation and hydrolysis products Penicillium multicolor alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
 ?
3.2.1.51 alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity Penicillium multicolor alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
 ?
3.2.1.51 alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine same yield of transglycosylation and hydrolysis products Penicillium multicolor alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
-
 ?
3.2.1.51 Fuc-alpha-(1-3)-GlcNAc + H2O slow hydrolysis rate Aspergillus niger alpha-L-fucose + N-acetyl-D-glucosamine
-
 ?
3.2.1.51 Fuc-alpha-(1-3)-GlcNAc + H2O slow hydrolysis rate Penicillium multicolor alpha-L-fucose + N-acetyl-D-glucosamine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 50
-
 stable up to Aspergillus niger
3.2.1.51 50
-
 stable up to Penicillium multicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 5
-
-
 Penicillium multicolor

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.51 3 7 stable within Penicillium multicolor