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Literature summary extracted from
- Purification and properties of alpha-amino-epsilon-caprolactam racemase from Achromobacter obae (1982), FEBS Lett., 150, 370-374.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.1.1.15 | freezing inactivates, particularly in dilute solution of less than 1 mg/ml | Achromobacter obae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.15 | hydroxylamine | 5 mM | Achromobacter obae |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.15 | 6 | - |
L-alpha-amino-epsilon-caprolactam | - |
Achromobacter obae | |
| 5.1.1.15 | 8 | - |
D-alpha-Amino-epsilon-caprolactam | - |
Achromobacter obae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.15 | 49000 | - |
1 * 49000, SDS-PAGE | Achromobacter obae |
| 5.1.1.15 | 51000 | - |
gel filtration | Achromobacter obae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.15 | Achromobacter obae | - |
FERM-P776 | - |
| 5.1.1.15 | Achromobacter obae FERM-P776 | - |
FERM-P776 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.1.15 | - |
Achromobacter obae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.15 | 380 | - |
- |
Achromobacter obae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.1.1.15 | -20°C, 0.25 M sucrose, enzyme concentration above 3 mg protein/ml, stable for at least 6 months | Achromobacter obae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.15 | L-alpha-Amino-epsilon-caprolactam | r | Achromobacter obae | D-alpha-Amino-epsilon-caprolactam | - |
? | |
| 5.1.1.15 | L-alpha-Amino-epsilon-caprolactam | r | Achromobacter obae FERM-P776 | D-alpha-Amino-epsilon-caprolactam | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.1.15 | monomer | 1 * 49000, SDS-PAGE | Achromobacter obae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.15 | 9 | - |
L-alpha-amino-epsilon-caprolactam | Achromobacter obae |
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