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Literature summary extracted from

  • Jwanny, E.W.; El-Sayed, S.T.; Rashad, M.M.; Mahmoud, A.E.; Abdallah, N.M.
    Myrosinase from roots of Raphanus sativus (1995), Phytochemistry, 39, 1301-1303.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.147 2-mercaptoethanol 1 mM, slight activation Raphanus sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.147 Ag+ 1 mM AgNO3, 79% inhibition Raphanus sativus
3.2.1.147 ascorbic acid
-
 Raphanus sativus
3.2.1.147 Ca2+ 1 mM CaSO4, 55% inhibition Raphanus sativus
3.2.1.147 Co2+ 1 mM CoNO3, 66% inhibition Raphanus sativus
3.2.1.147 Cu2+ 1 mM CuSO4, 30% inhibition Raphanus sativus
3.2.1.147 EDTA
-
 Raphanus sativus
3.2.1.147 Fe2+ 1 mM FeSO4, 61% inhibition Raphanus sativus
3.2.1.147 K2SO4 1 mM, complete inhibition Raphanus sativus
3.2.1.147 L-Cys
-
 Raphanus sativus
3.2.1.147 Mg2+ 1 mM MgSO4, 93% inhibition Raphanus sativus
3.2.1.147 Pb2+ 1 mM Pb-acetate, 60% inhibition Raphanus sativus
3.2.1.147 PCMB
-
 Raphanus sativus
3.2.1.147 Zn2+ 1 mM ZnSO4, 37% inhibition Raphanus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.147 0.47
-
 sinigrin
-
 Raphanus sativus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Raphanus sativus
-
 i.e. radish
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.147 2 isoenzymes Raphanus sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.147 leaf
-
 Raphanus sativus
-
3.2.1.147 root
-
 Raphanus sativus
-
3.2.1.147 seed
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.147 additional information
-
-
 Raphanus sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 glucosinalbin + H2O
-
 Raphanus sativus ?
-
 ?
3.2.1.147 progoitrin + H2O
-
 Raphanus sativus additional information
-
 ?
3.2.1.147 sinigrin + H2O
-
 Raphanus sativus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.147 37
-
-
 Raphanus sativus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.147 30
-
 90 min, stable Raphanus sativus
3.2.1.147 40
-
 45 min, 18% loss of activity Raphanus sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.147 6 6.5 at 37°C Raphanus sativus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.147 5.2 6.5 pH 5.2: about 50% of maximal activity, pH 6.0-6.5: optimum Raphanus sativus