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Literature summary extracted from

  • Bones, A.M.; Rossiter, J.T.
    The myrosinase-glucosinolate system, its organisation and biochemistry (1996), Physiol. Plant., 97, 194-208.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.147 ascorbic acid
-
 Lepidium sativum
3.2.1.147 ascorbic acid
-
 Brassica juncea
3.2.1.147 ascorbic acid activates Brassica napus
3.2.1.147 ascorbic acid activates Raphanus sativus
3.2.1.147 ascorbic acid activates Sinapis alba
3.2.1.147 ascorbic acid activates Eutrema japonicum
3.2.1.147 ascorbic acid maximal activation at 0.7 mM Sinapis alba
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Brassica oleracea
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Aspergillus sydowii
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Brassica rapa
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Sinapis alba
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Crambe hispanica subsp. abyssinica
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Armoracia rusticana
3.2.1.147 epithiospecifier protein interacts with myrosinase to promote sulfur transfer from the S-glucose moiety to the terminal alkenyl moiety. Degradation of progoitrin produces mainly oxazolidine-2-thione in the absence of epithiospecifier protein and mainly epithionitrile in the presence of epithiospecifier protein Crambe hispanica subsp. abyssinica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.147 extracellular
-
 Armoracia rusticana
-
-
3.2.1.147 membrane associated with the cytoplasmic side of internal membranes Armoracia rusticana 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Arabis alpina
-
-
-
3.2.1.147 Armoracia rusticana
-
-
-
3.2.1.147 Aspergillus niger
-
-
-
3.2.1.147 Aspergillus sydowii
-
-
-
3.2.1.147 Brassica juncea
-
-
-
3.2.1.147 Brassica napus
-
 i.e. oilseed rape
-
3.2.1.147 Brassica oleracea
-
 var. bullata subvar. gemmifera, i.e. Brussel sprouts
-
3.2.1.147 Brassica rapa
-
 var. chinensis
-
3.2.1.147 Brassica rapa subsp. chinensis
-
-
-
3.2.1.147 Brevicoryne brassicae
-
-
-
3.2.1.147 Crambe hispanica subsp. abyssinica
-
-
-
3.2.1.147 Enterobacter cloacae
-
-
-
3.2.1.147 Erysimum cheiri
-
-
-
3.2.1.147 Eutrema japonicum
-
-
-
3.2.1.147 Iberis umbellata
-
-
-
3.2.1.147 Lepidium sativum
-
-
-
3.2.1.147 Lipaphis erysimi
-
-
-
3.2.1.147 Paracolobactrum aerogenoides
-
-
-
3.2.1.147 Raphanus sativus
-
-
-
3.2.1.147 Sinapis alba
-
-
-
3.2.1.147 Tropaeolum majus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.147 cotyledon
-
 Brassica napus
-
3.2.1.147 cotyledon
-
 Sinapis alba
-
3.2.1.147 leaf
-
 Raphanus sativus
-
3.2.1.147 leaf
-
 Brassica rapa
-
3.2.1.147 leaf
-
 Brassica rapa subsp. chinensis
-
3.2.1.147 leaf activity is 4-5 times higher in the outer leaves than in the stalk, the inner leaves and the centre of the Brussels sprouts Brassica oleracea
-
3.2.1.147 myrosin cell
-
 Brassica napus
-
3.2.1.147 myrosin cell
-
 Raphanus sativus
-
3.2.1.147 myrosin cell
-
 Sinapis alba
-
3.2.1.147 seed
-
 Brassica napus
-
3.2.1.147 seed
-
 Raphanus sativus
-
3.2.1.147 seed
-
 Lepidium sativum
-
3.2.1.147 seed
-
 Brassica rapa
-
3.2.1.147 seed
-
 Sinapis alba
-
3.2.1.147 seed
-
 Brassica juncea
-
3.2.1.147 seed
-
 Crambe hispanica subsp. abyssinica
-
3.2.1.147 seedling
-
 Raphanus sativus
-
3.2.1.147 seedling
-
 Lepidium sativum
-
3.2.1.147 seedling root Sinapis alba
-
3.2.1.147 seedling cotyledon Brassica napus
-
3.2.1.147 seedling cotyledon Sinapis alba
-
3.2.1.147 seedling myrosinase gene MYR2 is transcribed in all organs of the seedling, myrosinase gene Myr1 shows no transcription in seedling Sinapis alba
-
3.2.1.147 seedling leaf Sinapis alba
-
3.2.1.147 seedling hypocotyl Sinapis alba
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 progoitrin + H2O
-
 Brassica napus additional information
-
 ?
3.2.1.147 progoitrin + H2O
-
 Raphanus sativus additional information
-
 ?
3.2.1.147 progoitrin + H2O
-
 Sinapis alba additional information
-
 ?
3.2.1.147 progoitrin + H2O
-
 Brevicoryne brassicae additional information
-
 ?
3.2.1.147 progoitrin + H2O
-
 Crambe hispanica subsp. abyssinica additional information mainly oxazolidine-2-thione in the absence of epithiospecifier protein and mainly epithionitrile in the presence of epithiospecifier protein ?
3.2.1.147 sinigrin + H2O
-
 Aspergillus niger D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Enterobacter cloacae D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Brassica napus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Raphanus sativus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Brassica oleracea D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Aspergillus sydowii D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Lepidium sativum D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Brassica rapa D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Sinapis alba D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Tropaeolum majus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Brassica juncea D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Paracolobactrum aerogenoides D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Crambe hispanica subsp. abyssinica D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Brassica rapa subsp. chinensis D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Armoracia rusticana D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Eutrema japonicum D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Brevicoryne brassicae D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Lipaphis erysimi D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Erysimum cheiri D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Arabis alpina D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Iberis umbellata D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.147 60
-
-
 Brassica napus
3.2.1.147 60
-
-
 Sinapis alba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.147 5.8
-
-
 Sinapis alba