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Literature summary extracted from

  • Wallis, G.L.F.; Hemming, F.W.; Peberdy, J.F.
    An extracellular beta-galactofuranosidase from Aspergillus niger and its use as a tool for glycoconjugate analysis (2001), Biochim. Biophys. Acta, 1525, 19-28.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.146 D-galactono-1,4-lactone
-
 Aspergillus fumigatus
3.2.1.146 D-galactono-1,4-lactone competitive inhibition, 50% inhibition in the presence of 1 mM ZnCl2, CaCl2, NiCl2 or AgNO3 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.146 3.87
-
 p-nitrophenyl beta-D-galactofuranoside
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.146 55000
-
 non-denaturing PAGE Aspergillus niger
3.2.1.146 90000
-
 SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.146 Aspergillus fumigatus
-
-
-
3.2.1.146 Aspergillus nidulans
-
-
-
3.2.1.146 Aspergillus niger
-
 enzyme is induced in the presence of galactofuranoside containing glycoconjugates
-
3.2.1.146 Aspergillus parasiticus
-
-
-
3.2.1.146 Aspergillus tamarii
-
-
-
3.2.1.146 Aspergillus versicolor
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.146 glycoprotein
-
 Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.146 dialysed culture filtrate, Sepharose-Q, NeobarAQ-1, Neobar AQ-2, Superose-12, GNA-agarose Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.146 26.3
-
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.146 p-nitrophenyl beta-D-galactofuranoside + H2O
-
 Aspergillus nidulans p-nitrophenol + D-galactose
-
 ?
3.2.1.146 p-nitrophenyl beta-D-galactofuranoside + H2O
-
 Aspergillus niger p-nitrophenol + D-galactose
-
 ?
3.2.1.146 p-nitrophenyl beta-D-galactofuranoside + H2O
-
 Aspergillus parasiticus p-nitrophenol + D-galactose
-
 ?
3.2.1.146 p-nitrophenyl beta-D-galactofuranoside + H2O
-
 Aspergillus fumigatus p-nitrophenol + D-galactose
-
 ?
3.2.1.146 p-nitrophenyl beta-D-galactofuranoside + H2O
-
 Aspergillus versicolor p-nitrophenol + D-galactose
-
 ?
3.2.1.146 p-nitrophenyl beta-D-galactofuranoside + H2O
-
 Aspergillus tamarii p-nitrophenol + D-galactose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.146 3 4 very little activity above pH 6.0 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.146 3 10
-
 Aspergillus niger