Literature summary extracted from

  • Li, Y.; Wang, L.; Pavlovat, N.V.; Li, S.; Lee, Y.C.
    alpha-KDOase activity in oyster and synthesis of alpha- and beta-4-methylumbelliferyl ketosides of 3-deoxy-D-manno-octulosonic acid (KDO) (1997), J. Biol. Chem., 272, 26419-26424.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.144 re-lipopolysaccharide + H2O Crassostrea virginica
-
3-deoxy-D-manno-octulosonic acid + lipopolysaccharide
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.144 Crassostrea virginica
-
oyster
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.144 Sephacryl S200, SP-Fractogel, Con A-Sepharose, octyl-Sepharose Crassostrea virginica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.144 hepatopancreas
-
Crassostrea virginica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.144 1433
-
-
Crassostrea virginica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.144 4-methylumbelliferyl 3-deoxy-alpha-D-manno-2-octulopyranosidonic acid + H2O
-
Crassostrea virginica 3-deoxy-D-manno-octulosonic acid + 4-methylumbelliferone
-
?
3.2.1.144 re-lipopolysaccharide + H2O
-
Crassostrea virginica 3-deoxy-D-manno-octulosonic acid + lipopolysaccharide
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.144 alpha-Kdo-ase enzyme releases alpha linked 3-deoxy-D-manno-octulosonic acid (KDO) from lipopolysaccharides Crassostrea virginica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.144 4.5
-
pH-optimum for natural substrate re-lipopolysaccharide, optimum for 4-methylumbelliferyl 3-deoxy-alpha-D-manno-2-octulopyranosidonic acid at pH 3 Crassostrea virginica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.144 3 8
-
Crassostrea virginica