Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sebastian, J.; Kolattukudy, P.E.
    Purification and characterization of cutinase from a fluorescent Pseudomonas putida bacterial strain isolated from phyllosphere (1988), Arch. Biochem. Biophys., 263, 77-85.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.74 (O,O)-diethyl-(3,5,6-trichloro)-2-pyridylphosphorothioate 1 mM, 90% inhibition Pseudomonas putida
3.1.1.74 alkylboronic acids
-
 Pseudomonas putida
3.1.1.74 diisopropyl fluorophosphate 1 mM, 90% inhibition Pseudomonas putida
3.1.1.74 phenylboronic acid 5 mM, 63% inhibition, competitive Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.74 0.21
-
 4-nitrophenyl hexanoate
-
 Pseudomonas putida
3.1.1.74 0.27
-
 p-nitrophenylbutanoate
-
 Pseudomonas putida
3.1.1.74 1.7
-
 4-nitrophenyl octanoate
-
 Pseudomonas putida
3.1.1.74 2.27
-
 p-nitrophenyltetradecanoate
-
 Pseudomonas putida
3.1.1.74 3.55
-
 p-nitrophenyldodecanoate
-
 Pseudomonas putida
3.1.1.74 3.98
-
 p-nitrophenyldecanoate
-
 Pseudomonas putida
3.1.1.74 4.54
-
 p-nitrophenylhexadecanoate
-
 Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.74 extracellular
-
 Pseudomonas putida
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.74 30000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.74 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.74
-
 Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.74 culture medium
-
 Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.74 7030
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.74 4-nitrophenyl dodecanoate + H2O
-
 Pseudomonas putida 4-nitrophenol + dodecanoate
-
 ?
3.1.1.74 4-nitrophenyl octanoate + H2O
-
 Pseudomonas putida 4-nitrophenol + octanoate
-
 ?
3.1.1.74 cutin + H2O apple cutin Pseudomonas putida additional information
-
 ?
3.1.1.74 additional information does not act on tripalmitoyl glycerol or trioleoyl glycerol Pseudomonas putida ?
-
 ?
3.1.1.74 p-nitrophenyl hexanoate + H2O
-
 Pseudomonas putida p-nitrophenol + hexanoate
-
 ?
3.1.1.74 p-nitrophenylbutanoate + H2O
-
 Pseudomonas putida p-nitrophenol + butanoate
-
 ?
3.1.1.74 p-nitrophenyldecanoate + H2O
-
 Pseudomonas putida p-nitrophenol + decanoate
-
 ?
3.1.1.74 p-nitrophenylhexadecanoate + H2O
-
 Pseudomonas putida p-nitrophenol + hexadecanoate
-
 ?
3.1.1.74 p-nitrophenyltetradecanoate + H2O
-
 Pseudomonas putida p-nitrophenol + tetradecanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.74 monomer 1 * 30000, SDS-PAGE Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.74 8.5 10.5
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.74 8 11 pH 8.0: about 60% of maximal activity, pH 11.0: about 50% of maximal activity Pseudomonas putida