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Literature summary extracted from

  • Blum, D.L.; Kataeva, I.A.; Li, X.L.; Ljungdahl, L.G.
    Feruloyl esterase activity of the Clostridium thermocellum cellulosome can be attributed to previously unknown domains of XynY and XynZ (2000), J. Bacteriol., 182, 1346-1351.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 domains XynY and XynZ containing homolgy sequences to Orpinomyces feruloyl esterase cloned and expressed in Escherichia coli Acetivibrio thermocellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 45000
-
 domain XynZ, expected product of the 1,203-bp gene Acetivibrio thermocellus
3.1.1.73 2000000
-
 cellulosome enzyme-complex Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 coastal bermuda grass + H2O Acetivibrio thermocellus
-
 ferulic acid + ?
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus niger
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Pseudomonas fluorescens
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Butyrivibrio fibrisolvens
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Orpinomyces sp.
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus tubingensis
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Acetivibrio thermocellus
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Neocallimastix sp.
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Ruminococcus sp.
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 wheat bran + H2O Aspergillus niger
-
 ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 wheat bran + H2O Acetivibrio thermocellus
-
 ferulic acid + p-coumaric acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Acetivibrio thermocellus
-
-
-
3.1.1.73 Acetivibrio thermocellus JW20
-
-
-
3.1.1.73 Aspergillus niger
-
-
-
3.1.1.73 Aspergillus tubingensis
-
-
-
3.1.1.73 Butyrivibrio fibrisolvens
-
-
-
3.1.1.73 Neocallimastix sp.
-
 N. patriciarum
-
3.1.1.73 no activity in Escherichia coli
-
-
-
3.1.1.73 Orpinomyces sp.
-
-
-
3.1.1.73 Pseudomonas fluorescens
-
 subsp. cellulosa
-
3.1.1.73 Ruminococcus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 recombinant protein Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 26.6
-
 FAE-XynZ Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabinofuranosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O
-
 Acetivibrio thermocellus ferulic acid + O-beta-D-xylopyranosyl-(1-2)-O-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
 ?
3.1.1.73 5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabinofuranosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O FAX3-I Neocallimastix sp. ferulic acid + O-beta-D-xylopyranosyl-(1-2)-O-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
 ?
3.1.1.73 5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabinofuranosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O
-
 Acetivibrio thermocellus JW20 ferulic acid + O-beta-D-xylopyranosyl-(1-2)-O-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
 ?
3.1.1.73 coastal bermuda grass + H2O
-
 Acetivibrio thermocellus ferulic acid + ?
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Aspergillus niger ferulic acid + ethanol
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Acetivibrio thermocellus ferulic acid + ethanol
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Acetivibrio thermocellus JW20 ferulic acid + ethanol
-
 ?
3.1.1.73 ethyl p-coumarate + H2O
-
 Acetivibrio thermocellus p-coumaric acid + ethanol
-
 ?
3.1.1.73 ethyl p-coumarate + H2O
-
 Acetivibrio thermocellus JW20 p-coumaric acid + ethanol
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Aspergillus niger ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Pseudomonas fluorescens ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Butyrivibrio fibrisolvens ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Orpinomyces sp. ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Aspergillus tubingensis ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Acetivibrio thermocellus ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Neocallimastix sp. ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Ruminococcus sp. ferulate + polysaccharide
-
 ?
3.1.1.73 additional information
-
 Aspergillus niger ?
-
 ?
3.1.1.73 additional information
-
 Pseudomonas fluorescens ?
-
 ?
3.1.1.73 additional information
-
 Neocallimastix sp. ?
-
 ?
3.1.1.73 additional information enzyme lacks activity towards carboxymethylcellulose, avicel, p-nitrophenyl(pNP)-arabinopyranoside, pNP-glucopyranoside, pNP-xylopyranoside and pNP-acetate Acetivibrio thermocellus ?
-
 ?
3.1.1.73 additional information enzyme lacks activity towards carboxymethylcellulose, avicel, p-nitrophenyl(pNP)-arabinopyranoside, pNP-glucopyranoside, pNP-xylopyranoside and pNP-acetate Acetivibrio thermocellus JW20 ?
-
 ?
3.1.1.73 O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O FAXX Acetivibrio thermocellus ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
 ?
3.1.1.73 O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O FAXX Neocallimastix sp. ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
 ?
3.1.1.73 O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O FAXX Acetivibrio thermocellus JW20 ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
 ?
3.1.1.73 wheat bran + H2O
-
 Aspergillus niger ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 wheat bran + H2O
-
 Acetivibrio thermocellus ferulic acid + p-coumaric acid + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 cellulosome multi-enzyme complex
-
 Acetivibrio thermocellus
3.1.1.73 cellulosome xylanase Z feruloyl esterase
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 50 60
-
 Acetivibrio thermocellus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73
-
 70 recombinant enzyme Acetivibrio thermocellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 70 90 stable for 6h, loses 50% activity at 80°C within 3 h, loses all activity after 1 h at 90°C Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 4 7 recombinant enzyme Acetivibrio thermocellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 3 10
-
 Acetivibrio thermocellus