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Literature summary extracted from
- Purification and characterization of a feruloyl esterase from the fungus Penicillium expansum (1997), J. Appl. Microbiol., 83, 718-726.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.1.73 | nutrition | enzymes capable of cleaving phenolic acid-ester cross-linkages may find applications in the food industry, they may enable enhanced extraction of plant material and the textural modification of food products, for example baking and other food processing industries, animal nutrition may benefit from the use of phenolic acid esterases | Penicillium expansum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.73 | Cu2+ | - |
Penicillium expansum |  |
| 3.1.1.73 | Cu2+ | - |
Talaromyces pinophilus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.73 | 2.6 | - |
methyl ferulate | - |
Penicillium expansum | |
| 3.1.1.73 | 2.9 | - |
methyl coumarate | p-coumaroyl esterase | Penicillium expansum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.73 | extracellular | - |
Streptomyces olivochromogenes | - |
- |
| 3.1.1.73 | extracellular | - |
Penicillium expansum | - |
- |
| 3.1.1.73 | extracellular | - |
Talaromyces pinophilus | - |
- |
| 3.1.1.73 | extracellular | - |
Neocallimastix sp. | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.73 | Fe3+ | 1 mM, residual activity 112% | Penicillium expansum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.73 | 57500 | - |
gel filtration, denaturing PAGE | Penicillium expansum |
| 3.1.1.73 | 65000 | - |
gel filtration, non-denaturing PAGE | Penicillium expansum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.73 | feruloyl-polysaccharide + H2O | Aspergillus niger | - |
ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | feruloyl-polysaccharide + H2O | Streptomyces olivochromogenes | - |
ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | feruloyl-polysaccharide + H2O | Fibrobacter succinogenes | - |
ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | feruloyl-polysaccharide + H2O | Schizophyllum commune | - |
ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | feruloyl-polysaccharide + H2O | Penicillium expansum | - |
ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | feruloyl-polysaccharide + H2O | Talaromyces pinophilus | - |
ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | feruloyl-polysaccharide + H2O | Neocallimastix sp. | - |
ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | italian rye gras cell walls + H2O | Penicillium expansum | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
| 3.1.1.73 | italian rye gras cell walls + H2O | Talaromyces pinophilus | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
| 3.1.1.73 | pectins + H2O | Aspergillus niger | sugar beet and spinach | ferulic acid + ? | - |
? | |
| 3.1.1.73 | pectins + H2O | Penicillium expansum | sugar beet and spinach | ferulic acid + ? | - |
? | |
| 3.1.1.73 | wheat bran + H2O | Aspergillus niger | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
| 3.1.1.73 | wheat bran + H2O | Penicillium expansum | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.73 | Aspergillus niger | - |
- |
- |
| 3.1.1.73 | Fibrobacter succinogenes | - |
- |
- |
| 3.1.1.73 | Neocallimastix sp. | - |
- |
- |
| 3.1.1.73 | Penicillium expansum | - |
- |
- |
| 3.1.1.73 | Schizophyllum commune | - |
- |
- |
| 3.1.1.73 | Streptomyces olivochromogenes | - |
- |
- |
| 3.1.1.73 | Talaromyces pinophilus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.73 | - |
Penicillium expansum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.73 | 3.2 | - |
PAXX as substrate | Penicillium expansum |
| 3.1.1.73 | 5.3 | - |
2-O-5-O-(transferuloyl)-beta-L-arabinofuranosyl-D-xylopyranose as substrate | Penicillium expansum |
| 3.1.1.73 | 8.06 | - |
methyl caffeate as substrate | Penicillium expansum |
| 3.1.1.73 | 9.6 | - |
FAXX as substrate | Penicillium expansum |
| 3.1.1.73 | 10.3 | - |
methyl coumarate as substrate | Penicillium expansum |
| 3.1.1.73 | 12.7 | - |
o-nitrophenol acetate as substrate | Penicillium expansum |
| 3.1.1.73 | 14.4 | - |
feruloyl/p-coumaroyl esterase, methyl ferulate as substrate | Penicillium expansum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.73 | -20°C, approximately 78% activity retained after storage for 2 months | Penicillium expansum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.73 | 2-nitrophenyl acetate + H2O | - |
Penicillium expansum | 2-nitrophenol + acetate | - |
? | |
| 3.1.1.73 | 2-O-5-O-(trans-feruloyl)-beta-L-arabinofuranosyl-D-xylopyranose + H2O | FAX | Penicillium expansum | ferulic acid + 2-O-beta-L-arabinofuranosyl-D-xylopyranose | - |
? | |
| 3.1.1.73 | ferulic acid methyl ester + H2O | - |
Aspergillus niger | ferulic acid + methanol | - |
? | |
| 3.1.1.73 | ferulic acid methyl ester + H2O | - |
Streptomyces olivochromogenes | ferulic acid + methanol | - |
? | |
| 3.1.1.73 | ferulic acid methyl ester + H2O | - |
Penicillium expansum | ferulic acid + methanol | - |
? | |
| 3.1.1.73 | ferulic acid methyl ester + H2O | - |
Talaromyces pinophilus | ferulic acid + methanol | - |
? | |
| 3.1.1.73 | ferulic acid methyl ester + H2O | - |
Neocallimastix sp. | ferulic acid + methanol | - |
? | |
| 3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Aspergillus niger | ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Streptomyces olivochromogenes | ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Fibrobacter succinogenes | ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Schizophyllum commune | ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Penicillium expansum | ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Talaromyces pinophilus | ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Neocallimastix sp. | ferulate + polysaccharide | - |
? | |
| 3.1.1.73 | italian rye gras cell walls + H2O | - |
Penicillium expansum | ferulic acid + p-coumaric acid + ? | - |
? | |
| 3.1.1.73 | italian rye gras cell walls + H2O | - |
Talaromyces pinophilus | ferulic acid + p-coumaric acid + ? | - |
? | |
| 3.1.1.73 | methyl caffeate + H2O | - |
Aspergillus niger | caffeic acid + methanol | - |
? | |
| 3.1.1.73 | methyl caffeate + H2O | - |
Penicillium expansum | caffeic acid + methanol | - |
? | |
| 3.1.1.73 | methyl coumarate + H2O | - |
Penicillium expansum | coumaric acid + methanol | - |
? | |
| 3.1.1.73 | additional information | - |
Aspergillus niger | ? | - |
? | |
| 3.1.1.73 | additional information | - |
Streptomyces olivochromogenes | ? | - |
? | |
| 3.1.1.73 | additional information | - |
Talaromyces pinophilus | ? | - |
? | |
| 3.1.1.73 | additional information | - |
Neocallimastix sp. | ? | - |
? | |
| 3.1.1.73 | additional information | no activity on methyl sinapinate, methyl syringate, methyl vanillate, p-nitrophenyl-alpha-L-arabinofuranoside, p-nitrophenyl-beta-D-xylopyranoside, oat spelt xylan, sodium polypectate and citrus pectin | Penicillium expansum | ? | - |
? | |
| 3.1.1.73 | O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAXX | Penicillium expansum | ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| 3.1.1.73 | O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAXX | Neocallimastix sp. | ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| 3.1.1.73 | O-5-O-[(E)-p-coumaroyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | PAXX | Penicillium expansum | p-coumaric acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| 3.1.1.73 | O-5-O-[(E)-p-coumaroyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | PAXX | Neocallimastix sp. | p-coumaric acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| 3.1.1.73 | p-nitrophenyl butyrate + H2O | - |
Aspergillus niger | p-nitrophenol + butyric acid | - |
? | |
| 3.1.1.73 | p-nitrophenyl butyrate + H2O | - |
Penicillium expansum | p-nitrophenol + butyric acid | - |
? | |
| 3.1.1.73 | pectins + H2O | sugar beet and spinach | Aspergillus niger | ferulic acid + ? | - |
? | |
| 3.1.1.73 | pectins + H2O | sugar beet and spinach | Penicillium expansum | ferulic acid + ? | - |
? | |
| 3.1.1.73 | wheat bran + H2O | - |
Aspergillus niger | ferulic acid + p-coumaric acid + ? | - |
? | |
| 3.1.1.73 | wheat bran + H2O | - |
Penicillium expansum | ferulic acid + p-coumaric acid + ? | - |
? | |
| 3.1.1.73 | xylan polysaccharide + H2O | - |
Aspergillus niger | ferulic acid + p-coumaric acid | - |
? | |
| 3.1.1.73 | xylan polysaccharide + H2O | - |
Penicillium expansum | ferulic acid + p-coumaric acid | - |
? | |
| 3.1.1.73 | xylan polysaccharide + H2O | - |
Talaromyces pinophilus | ferulic acid + p-coumaric acid | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.73 | 37 | - |
- |
Penicillium expansum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.73 | 5.6 | - |
- |
Penicillium expansum |
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