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Literature summary extracted from

  • Borneman, W.S.; Ljungdahl, L.G.; Hartley, R.D.; Akin, D.E.
    Purification and partial characterization of two feruloyl esterases from the anaerobic fungus Neocallimastix strain MC-2 (1992), Appl. Environ. Microbiol., 58, 3762-3766.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.0096
-
 O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose FAE-II Neocallimastix sp.
3.1.1.73 0.0319
-
 O-5-O-(E)-feruloyl-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose FAE-I Neocallimastix sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Streptomyces olivochromogenes
-
-
3.1.1.73 extracellular
-
 Neocallimastix sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 24000
-
 FAE-II, denaturing and nondenaturing PAGE, gel filtration, high performance gel filtration Neocallimastix sp.
3.1.1.73 29000
-
-
 Streptomyces olivochromogenes
3.1.1.73 68000
-
 FAE-I, high-performance gel filtration Neocallimastix sp.
3.1.1.73 69000
-
 FAE-I, denaturing and nondenaturing PAGE, gel filtration Neocallimastix sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus niger
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Streptomyces olivochromogenes
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Schizophyllum commune
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Neocallimastix sp.
-
 ferulate + polysaccharide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus niger
-
-
-
3.1.1.73 Neocallimastix sp.
-
 2 extracellular feruloyl esterases, FAE-I and FAE-II
-
3.1.1.73 Neocallimastix sp.
-
 rumen fungus, MC-2
-
3.1.1.73 Schizophyllum commune
-
-
-
3.1.1.73 Streptomyces olivochromogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
 Streptomyces olivochromogenes
3.1.1.73 p-coumaroyl esterase, FAE-I, FAE-II Neocallimastix sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 3.9
-
 FAE-I Neocallimastix sp.
3.1.1.73 11.2
-
 FAE-II Neocallimastix sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabinofuranosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O FAX3-I Neocallimastix sp. ferulic acid + O-beta-D-xylopyranosyl-(1-2)-O-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
 ?
3.1.1.73 de-starched wheat bran + H2O
-
 Aspergillus niger ferulic acid + ?
-
 ?
3.1.1.73 de-starched wheat bran + H2O
-
 Streptomyces olivochromogenes ferulic acid + ?
-
 ?
3.1.1.73 de-starched wheat bran + H2O
-
 Schizophyllum commune ferulic acid + ?
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Aspergillus niger ferulic acid + methanol
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Streptomyces olivochromogenes ferulic acid + methanol
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Neocallimastix sp. ferulic acid + methanol
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Aspergillus niger ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Streptomyces olivochromogenes ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Schizophyllum commune ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Neocallimastix sp. ferulate + polysaccharide
-
 ?
3.1.1.73 O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O FAXX Neocallimastix sp. ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
 ?
3.1.1.73 O-5-O-[(E)-p-coumaroyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O PAXX Neocallimastix sp. p-coumaric acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
 ?
3.1.1.73 O-beta-D-xylopyranosyl-(1-4)-O-[5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O FAX3-II Neocallimastix sp. ferulic acid + O-beta-D-xylopyranosyl-(1-4)-[O-alpha-L-arabinofuranosyl-(1-3)]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 monomer
-
 Streptomyces olivochromogenes
3.1.1.73 monomer 1 * 68000, SDS-PAGE, FAE-I Neocallimastix sp.
3.1.1.73 monomer 1 * 24000, SDS-PAGE, FAE-II Neocallimastix sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 40
-
 both FAE-I and FAE-II relatively stable Neocallimastix sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6.2
-
-
 Neocallimastix sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 5.5 6.8 FAE-I most active in this range Neocallimastix sp.
3.1.1.73 6.4 7.6 FAE-II most active in this range Neocallimastix sp.