EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.73 | 1.86 | - |
methyl ferulate | - |
Streptomyces olivochromogenes |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.73 | extracellular | - |
Streptomyces olivochromogenes | - |
- |
3.1.1.73 | extracellular | - |
Neocallimastix sp. | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 29000 | - |
gel filtration | Streptomyces olivochromogenes |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | feruloyl-polysaccharide + H2O | Aspergillus niger | - |
ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | Streptomyces olivochromogenes | - |
ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | Schizophyllum commune | - |
ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | Neocallimastix sp. | - |
ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | Streptomyces olivochromogenes NRCC 2258 | - |
ferulate + polysaccharide | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.73 | Aspergillus niger | - |
- |
- |
3.1.1.73 | Neocallimastix sp. | - |
rumen fungus, MC-2 | - |
3.1.1.73 | Schizophyllum commune | - |
- |
- |
3.1.1.73 | Streptomyces olivochromogenes | - |
NRCC 2258 | - |
3.1.1.73 | Streptomyces olivochromogenes NRCC 2258 | - |
NRCC 2258 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.73 | - |
Streptomyces olivochromogenes |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 0.19 | - |
methyl ferulate as substrate | Streptomyces olivochromogenes |
3.1.1.73 | 1.51 | - |
de-starched wheat bran as substrate | Streptomyces olivochromogenes |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.1.73 | -20°C, pH 7, enzyme loses activity over 24 h even in presence of EDTA, DTT, Mg2+ and Ca2+, additives which stabilizes the enzyme during purification | Streptomyces olivochromogenes |
3.1.1.73 | 4°C, pH 7, enzyme loses activity over 24 h even in presence of EDTA, DTT, Mg2+ and Ca2+, additives which stabilizes the enzyme during purification | Streptomyces olivochromogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | cinnamic acid methyl ester + H2O | - |
Aspergillus niger | cinnamic acid + methanol | - |
? | |
3.1.1.73 | cinnamic acid methyl ester + H2O | - |
Neocallimastix sp. | cinnamic acid + methanol | - |
? | |
3.1.1.73 | de-starched wheat bran + H2O | - |
Aspergillus niger | ferulic acid + ? | - |
? | |
3.1.1.73 | de-starched wheat bran + H2O | purified enzyme releases ferulic acid only in presence of xylanase | Streptomyces olivochromogenes | ferulic acid + ? | - |
? | |
3.1.1.73 | de-starched wheat bran + H2O | purified enzyme releases ferulic acid only in presence of xylanase | Schizophyllum commune | ferulic acid + ? | - |
? | |
3.1.1.73 | de-starched wheat bran + H2O | purified enzyme releases ferulic acid only in presence of xylanase | Streptomyces olivochromogenes NRCC 2258 | ferulic acid + ? | - |
? | |
3.1.1.73 | ferulic acid methyl ester + H2O | - |
Aspergillus niger | ferulic acid + methanol | - |
? | |
3.1.1.73 | ferulic acid methyl ester + H2O | - |
Streptomyces olivochromogenes | ferulic acid + methanol | - |
? | |
3.1.1.73 | ferulic acid methyl ester + H2O | - |
Neocallimastix sp. | ferulic acid + methanol | - |
? | |
3.1.1.73 | ferulic acid methyl ester + H2O | - |
Streptomyces olivochromogenes NRCC 2258 | ferulic acid + methanol | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Aspergillus niger | ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Streptomyces olivochromogenes | ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Schizophyllum commune | ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Neocallimastix sp. | ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Streptomyces olivochromogenes NRCC 2258 | ferulate + polysaccharide | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.73 | monomer | - |
Neocallimastix sp. |
3.1.1.73 | monomer | 1 * 29000, SDS-PAGE | Streptomyces olivochromogenes |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.73 | 4-hydroxy-3-methoxycinnamic acid esterase | - |
Streptomyces olivochromogenes |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 30 | - |
methyl ferulate as substrate | Streptomyces olivochromogenes |
3.1.1.73 | 50 | - |
- |
Neocallimastix sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 20 | 70 | - |
Aspergillus niger |
3.1.1.73 | 20 | 70 | - |
Streptomyces olivochromogenes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 5.5 | - |
methyl ferulate as substrate | Streptomyces olivochromogenes |
3.1.1.73 | 7.5 | - |
- |
Neocallimastix sp. |