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Literature summary extracted from

  • Faulds, C.B.; Williamson, G.
    The purification and characterization of 4-hydroxy-3-methoxycinnamic (ferulic) acid esterase from Streptomyces olivochromogenes (1991), J. Gen. Microbiol., 137, 2339-2345.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 1.86
-
methyl ferulate
-
Streptomyces olivochromogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
Streptomyces olivochromogenes
-
-
3.1.1.73 extracellular
-
Neocallimastix sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 29000
-
gel filtration Streptomyces olivochromogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus niger
-
ferulate + polysaccharide
-
?
3.1.1.73 feruloyl-polysaccharide + H2O Streptomyces olivochromogenes
-
ferulate + polysaccharide
-
?
3.1.1.73 feruloyl-polysaccharide + H2O Schizophyllum commune
-
ferulate + polysaccharide
-
?
3.1.1.73 feruloyl-polysaccharide + H2O Neocallimastix sp.
-
ferulate + polysaccharide
-
?
3.1.1.73 feruloyl-polysaccharide + H2O Streptomyces olivochromogenes NRCC 2258
-
ferulate + polysaccharide
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus niger
-
-
-
3.1.1.73 Neocallimastix sp.
-
rumen fungus, MC-2
-
3.1.1.73 Schizophyllum commune
-
-
-
3.1.1.73 Streptomyces olivochromogenes
-
NRCC 2258
-
3.1.1.73 Streptomyces olivochromogenes NRCC 2258
-
NRCC 2258
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
Streptomyces olivochromogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 0.19
-
methyl ferulate as substrate Streptomyces olivochromogenes
3.1.1.73 1.51
-
de-starched wheat bran as substrate Streptomyces olivochromogenes

Storage Stability

EC Number Storage Stability Organism
3.1.1.73 -20°C, pH 7, enzyme loses activity over 24 h even in presence of EDTA, DTT, Mg2+ and Ca2+, additives which stabilizes the enzyme during purification Streptomyces olivochromogenes
3.1.1.73 4°C, pH 7, enzyme loses activity over 24 h even in presence of EDTA, DTT, Mg2+ and Ca2+, additives which stabilizes the enzyme during purification Streptomyces olivochromogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 cinnamic acid methyl ester + H2O
-
Aspergillus niger cinnamic acid + methanol
-
?
3.1.1.73 cinnamic acid methyl ester + H2O
-
Neocallimastix sp. cinnamic acid + methanol
-
?
3.1.1.73 de-starched wheat bran + H2O
-
Aspergillus niger ferulic acid + ?
-
?
3.1.1.73 de-starched wheat bran + H2O purified enzyme releases ferulic acid only in presence of xylanase Streptomyces olivochromogenes ferulic acid + ?
-
?
3.1.1.73 de-starched wheat bran + H2O purified enzyme releases ferulic acid only in presence of xylanase Schizophyllum commune ferulic acid + ?
-
?
3.1.1.73 de-starched wheat bran + H2O purified enzyme releases ferulic acid only in presence of xylanase Streptomyces olivochromogenes NRCC 2258 ferulic acid + ?
-
?
3.1.1.73 ferulic acid methyl ester + H2O
-
Aspergillus niger ferulic acid + methanol
-
?
3.1.1.73 ferulic acid methyl ester + H2O
-
Streptomyces olivochromogenes ferulic acid + methanol
-
?
3.1.1.73 ferulic acid methyl ester + H2O
-
Neocallimastix sp. ferulic acid + methanol
-
?
3.1.1.73 ferulic acid methyl ester + H2O
-
Streptomyces olivochromogenes NRCC 2258 ferulic acid + methanol
-
?
3.1.1.73 feruloyl-polysaccharide + H2O
-
Aspergillus niger ferulate + polysaccharide
-
?
3.1.1.73 feruloyl-polysaccharide + H2O
-
Streptomyces olivochromogenes ferulate + polysaccharide
-
?
3.1.1.73 feruloyl-polysaccharide + H2O
-
Schizophyllum commune ferulate + polysaccharide
-
?
3.1.1.73 feruloyl-polysaccharide + H2O
-
Neocallimastix sp. ferulate + polysaccharide
-
?
3.1.1.73 feruloyl-polysaccharide + H2O
-
Streptomyces olivochromogenes NRCC 2258 ferulate + polysaccharide
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 monomer
-
Neocallimastix sp.
3.1.1.73 monomer 1 * 29000, SDS-PAGE Streptomyces olivochromogenes

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 4-hydroxy-3-methoxycinnamic acid esterase
-
Streptomyces olivochromogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 30
-
methyl ferulate as substrate Streptomyces olivochromogenes
3.1.1.73 50
-
-
Neocallimastix sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 20 70
-
Aspergillus niger
3.1.1.73 20 70
-
Streptomyces olivochromogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5.5
-
methyl ferulate as substrate Streptomyces olivochromogenes
3.1.1.73 7.5
-
-
Neocallimastix sp.