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Literature summary extracted from

  • Narbad, A.; Gasson, M.J.
    Metabolism of ferulic acid via vanillin using a novel CoA-dependent pathway in a newly isolated strain of Pseudomonas fluorescens (1998), Microbiology, 144, 1397-1405.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.34 Mg2+ required Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.67 4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O Pseudomonas fluorescens no reduction of vanillic acid to vanillin in crude cell extract observed, metabolism of ferulic acid to vanillic acid 4-hydroxy-3-methoxybenzoate + NADH + H+
-
 ir
1.2.1.67 4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O Pseudomonas fluorescens AN103 no reduction of vanillic acid to vanillin in crude cell extract observed, metabolism of ferulic acid to vanillic acid 4-hydroxy-3-methoxybenzoate + NADH + H+
-
 ir
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA Bacillus subtilis
-
 vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA Escherichia coli
-
 vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA Pseudomonas fluorescens
-
 vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA Corynebacterium glutamicum
-
 vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA Streptomyces griseus
-
 vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA Delftia acidovorans
-
 vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA Burkholderia cepacia
-
 vanillin + acetyl-CoA
-
 ?
4.1.2.61 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA Pseudomonas fluorescens
-
 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA Pseudomonas fluorescens AN103
-
 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 trans-feruloyl-CoA + H2O Pseudomonas fluorescens
-
 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?
4.1.2.61 trans-feruloyl-CoA + H2O Pseudomonas fluorescens AN103
-
 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?
6.2.1.34 ferulic acid + CoASH + ATP Pseudomonas fluorescens enzyme is involved in ferulic acid metabolism, inducible enzyme trans-feruloyl-CoA + products of ATP breakdown
-
 ?
6.2.1.34 ferulic acid + CoASH + ATP Pseudomonas fluorescens AN103 enzyme is involved in ferulic acid metabolism, inducible enzyme trans-feruloyl-CoA + products of ATP breakdown
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.67 Pseudomonas fluorescens
-
-
-
4.1.2.61 Bacillus subtilis
-
-
-
4.1.2.61 Burkholderia cepacia
-
-
-
4.1.2.61 Corynebacterium glutamicum
-
-
-
4.1.2.61 Delftia acidovorans
-
-
-
4.1.2.61 Escherichia coli
-
-
-
4.1.2.61 Pseudomonas fluorescens
-
-
-
4.1.2.61 Streptomyces griseus
-
-
-
6.2.1.34 Pseudomonas fluorescens
-
 AN103
-
6.2.1.34 Pseudomonas fluorescens AN103
-
 AN103
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.67 cell culture maximal enzyme synthesis, if cells grown in ferulic acid containing medium Pseudomonas fluorescens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.67 4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O no reduction of vanillic acid to vanillin in crude cell extract observed, metabolism of ferulic acid to vanillic acid Pseudomonas fluorescens 4-hydroxy-3-methoxybenzoate + NADH + H+
-
 ir
1.2.1.67 4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O no reduction of vanillic acid to vanillin in crude cell extract observed, metabolism of ferulic acid to vanillic acid Pseudomonas fluorescens AN103 4-hydroxy-3-methoxybenzoate + NADH + H+
-
 ir
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Pseudomonas fluorescens 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Bacillus subtilis vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Escherichia coli vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Pseudomonas fluorescens vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Corynebacterium glutamicum vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Streptomyces griseus vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Delftia acidovorans vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Burkholderia cepacia vanillin + acetyl-CoA
-
 ?
4.1.2.61 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 Pseudomonas fluorescens 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 Pseudomonas fluorescens AN103 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 trans-feruloyl-CoA + H2O
-
 Pseudomonas fluorescens 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?
4.1.2.61 trans-feruloyl-CoA + H2O
-
 Pseudomonas fluorescens AN103 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?
6.2.1.34 ferulic acid + CoASH + ATP
-
 Pseudomonas fluorescens trans-feruloyl-CoA + products of ATP breakdown
-
 ?
6.2.1.34 ferulic acid + CoASH + ATP enzyme is involved in ferulic acid metabolism, inducible enzyme Pseudomonas fluorescens trans-feruloyl-CoA + products of ATP breakdown
-
 ?
6.2.1.34 ferulic acid + CoASH + ATP
-
 Pseudomonas fluorescens AN103 trans-feruloyl-CoA + products of ATP breakdown
-
 ?
6.2.1.34 ferulic acid + CoASH + ATP enzyme is involved in ferulic acid metabolism, inducible enzyme Pseudomonas fluorescens AN103 trans-feruloyl-CoA + products of ATP breakdown
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.61 4-hydroxycinnamoyl-CoA hydratase/lyase
-
 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.67 NAD+ in absence of NAD+ only 25% oxidation of vanillin observed Pseudomonas fluorescens