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Literature summary extracted from

  • Bauer, I.; Max, N.; Fetzner, S.; Lingens, F.
    2,4-Dioxygenases catalyzing N-heterocyclic-ring cleavage and formation of carbon monoxide. Purification and some properties of 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase from Arthrobacter sp. Rii61a and comparison with 1H-3-hydroxy-4-oxoquinoline 2,4-dioxygenase from Pseudomonas putida 33/1 (1996), Eur. J. Biochem., 240, 576-583.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.47 ascorbate
-
 Arthrobacter sp.
1.13.11.47 Ca2+ weak Arthrobacter sp.
1.13.11.47 Co2+ weak Arthrobacter sp.
1.13.11.47 Cu2+ strong Arthrobacter sp.
1.13.11.47 Cu2+
-
 Pseudomonas putida
1.13.11.47 ethylxanthate
-
 Arthrobacter sp.
1.13.11.47 Fe2+ strong Arthrobacter sp.
1.13.11.47 H2O2
-
 Arthrobacter sp.
1.13.11.47 H2O2
-
 Pseudomonas putida
1.13.11.47 iodoacetamide
-
 Arthrobacter sp.
1.13.11.47 Mg2+ weak Arthrobacter sp.
1.13.11.47 Mn2+ weak Arthrobacter sp.
1.13.11.47 NEM
-
 Arthrobacter sp.
1.13.11.47 Ni2+ strong Arthrobacter sp.
1.13.11.47 p-hydroxymercuribenzoate
-
 Arthrobacter sp.
1.13.11.47 Sodium azide
-
 Arthrobacter sp.
1.13.11.47 Sodium dithionite
-
 Arthrobacter sp.
1.13.11.47 Zn2+ strong Arthrobacter sp.
1.13.11.47 Zn2+
-
 Pseudomonas putida
1.13.11.48 Ca2+ 1 mM, weak inhibition Arthrobacter sp.
1.13.11.48 Co2+ 1 mM, weak inhibition Arthrobacter sp.
1.13.11.48 Cu2+ 1 mM, strong inhibition Arthrobacter sp.
1.13.11.48 Fe2+ 1 mM, strong inhibition Arthrobacter sp.
1.13.11.48 Mg2+ 1 mM, weak inhibition Arthrobacter sp.
1.13.11.48 Mn2+ 1 mM, weak inhibition Arthrobacter sp.
1.13.11.48 Ni2+ 1 mM, strong inhibition Arthrobacter sp.
1.13.11.48 Zn2+ 1 mM, strong inhibition Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.47 0.024
-
 1H-3-Hydroxy-4-oxoquinoline
-
 Pseudomonas putida
1.13.11.47 0.03
-
 1H-3-Hydroxy-4-oxoquinaldine
-
 Arthrobacter sp.
1.13.11.48 0.03
-
 1H-3-Hydroxy-4-oxoquinaldine
-
 Arthrobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.47 copper contains 0.048 mol of Cu per mol of enzyme Pseudomonas putida
1.13.11.47 additional information addition of metal ions in the absence and in the presence of the reductant ascorbate does not increase activity Pseudomonas putida
1.13.11.47 additional information addition of metal ions in the absence and in the presence of the reductant ascorbate does not increase activity Arthrobacter sp.
1.13.11.47 Nickel contains 0.038 mol of Ni per mol of enzyme Pseudomonas putida
1.13.11.47 Nickel contains 0.02 mol of Ni per mol of enzyme Arthrobacter sp.
1.13.11.47 Zinc contains 0.045 mol of zinc per mol of enzyme Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.47 30000
-
 gel filtration Pseudomonas putida
1.13.11.47 32000
-
 gel filtration Arthrobacter sp.
1.13.11.48 32000
-
 gel filtration Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.47 Arthrobacter sp.
-
 strain R³61a
-
1.13.11.47 Arthrobacter sp. Ru61a
-
 strain R³61a
-
1.13.11.47 Pseudomonas putida
-
-
-
1.13.11.47 Pseudomonas putida 33/1
-
-
-
1.13.11.48 Arthrobacter sp.
-
 Ru61a
-
1.13.11.48 Arthrobacter sp. Ru61a
-
 Ru61a
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.47
-
 Pseudomonas putida
1.13.11.47
-
 Arthrobacter sp.
1.13.11.48
-
 Arthrobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.47 culture condition:quinaldine-grown cell
-
 Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.47 13.1
-
-
 Pseudomonas putida
1.13.11.47 70.7
-
-
 Arthrobacter sp.
1.13.11.48 70.7
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.47 1H-3-Hydroxy-4-oxoquinaldine + O2
-
 Arthrobacter sp. N-Acetylanthranilate + CO
-
 ?
1.13.11.47 1H-3-Hydroxy-4-oxoquinaldine + O2
-
 Arthrobacter sp. Ru61a N-Acetylanthranilate + CO
-
 ?
1.13.11.47 1H-3-Hydroxy-4-oxoquinoline + O2
-
 Pseudomonas putida N-Formylanthranilate + CO
-
 ?
1.13.11.47 1H-3-Hydroxy-4-oxoquinoline + O2 at 20% of the activity with 1H-3-Hydroxy-4-oxoquinaldine Arthrobacter sp. N-Formylanthranilate + CO
-
 ?
1.13.11.47 1H-3-Hydroxy-4-oxoquinoline + O2
-
 Pseudomonas putida 33/1 N-Formylanthranilate + CO
-
 ?
1.13.11.47 1H-3-Hydroxy-4-oxoquinoline + O2 at 20% of the activity with 1H-3-Hydroxy-4-oxoquinaldine Arthrobacter sp. Ru61a N-Formylanthranilate + CO
-
 ?
1.13.11.48 1H-3-hydroxy-4-oxoquinaldine + O2 1H-3-hydroxy-4-oxoquinaldine oxygenase Arthrobacter sp. N-acetylanthranilic acid + CO
-
 ir
1.13.11.48 1H-3-hydroxy-4-oxoquinaldine + O2 1H-3-hydroxy-4-oxoquinaldine oxygenase Arthrobacter sp. Ru61a N-acetylanthranilic acid + CO
-
 ir
1.13.11.48 1H-3-hydroxy-4-oxoquinoline + O2 also 1H-3-hydroxy-4-oxoquinaldine oxygenase Arthrobacter sp. N-formylanthranilic acid + CO
-
 ir
1.13.11.48 1H-3-hydroxy-4-oxoquinoline + O2 also 1H-3-hydroxy-4-oxoquinaldine oxygenase Arthrobacter sp. Ru61a N-formylanthranilic acid + CO
-
 ir

Subunits

EC Number Subunits Comment Organism
1.13.11.47 monomer 1 * 30000, SDS-PAGE Pseudomonas putida
1.13.11.47 monomer 1 * 32000, SDS-PAGE Arthrobacter sp.
1.13.11.48 monomer SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.47 25
-
-
 Pseudomonas putida
1.13.11.47 30
-
-
 Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.47 30
-
 labile above Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.47 8
-
-
 Pseudomonas putida
1.13.11.47 8
-
-
 Arthrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.47 additional information no spectral evidence for the presence of a chromophoric cofactor Pseudomonas putida
1.13.11.47 additional information no spectral evidence for the presence of a chromophoric cofactor Arthrobacter sp.
1.13.11.48 additional information no cofactors Arthrobacter sp.