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Literature summary extracted from

  • Inagaki, K.; Tanizawa, K.; Tanaka, H.; Soda, K.
    Purification and characterization of amino acid racemase with very broad substrate specificity from Aeromonas caviae (1987), Agric. Biol. Chem., 51, 173-180.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.10 hydroxylamine
-
Aeromonas caviae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.10 0.83
-
D-Lys
-
Aeromonas caviae
5.1.1.10 0.9
-
L-Orn
-
Aeromonas caviae
5.1.1.10 1
-
D-Ser
-
Aeromonas caviae
5.1.1.10 1.05
-
L-Lys
-
Aeromonas caviae
5.1.1.10 1.2
-
L-Met
-
Aeromonas caviae
5.1.1.10 1.6
-
D-Met
-
Aeromonas caviae
5.1.1.10 2.5
-
L-Lys D-Lys Aeromonas caviae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.10 40000
-
2 * 40000, SDS-PAGE Aeromonas caviae
5.1.1.10 73000
-
gel filtration Aeromonas caviae
5.1.1.10 76000
-
sedimentation equilibrium method Aeromonas caviae

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.10 Aeromonas caviae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.10
-
Aeromonas caviae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.10 1250
-
L-Lys as substrate Aeromonas caviae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.10 D-Arg 65% of the activity relative to L-Lys Aeromonas caviae L-Arg
-
?
5.1.1.10 D-Ser
-
Aeromonas caviae L-Ser
-
r
5.1.1.10 L-Asn 9% of the activity relative to L-Lys Aeromonas caviae D-Asn
-
?
5.1.1.10 L-Citrulline 30% of the activity relative to L-Lys Aeromonas caviae D-Citrulline
-
?
5.1.1.10 L-Ethionine 67% of the activity relative to L-Lys Aeromonas caviae D-Ethionine
-
?
5.1.1.10 L-Glu 57% of the activity relative to L-Lys Aeromonas caviae D-Glu
-
?
5.1.1.10 L-Homoarginine 67% of the activity relative to L-Lys Aeromonas caviae L-Homoarginine
-
?
5.1.1.10 L-Homocitrulline 34% of the activity relative to L-Lys Aeromonas caviae D-Homocitrulline
-
?
5.1.1.10 L-Homocysteine 55% of the activity relative to L-Lys Aeromonas caviae D-Homocysteine
-
?
5.1.1.10 L-homoserine 12% of the activity relative to L-Lys Aeromonas caviae D-homoserine
-
?
5.1.1.10 L-Leu 12% of the activity relative to L-Lys Aeromonas caviae D-Leu
-
?
5.1.1.10 L-Lys
-
Aeromonas caviae D-Lys
-
?
5.1.1.10 L-Met 67% of the activity relative to L-Lys Aeromonas caviae D-Met
-
?
5.1.1.10 L-Norleucine 28% of the activity relative to L-Lys Aeromonas caviae D-Norleucine
-
?
5.1.1.10 L-Orn 79% of the activity relative to L-Lys Aeromonas caviae D-Orn
-
?
5.1.1.10 L-Selenohomocysteine 27% of the activity relative to L-Lys Aeromonas caviae D-Selenohomocysteine
-
?
5.1.1.10 N6-Acetyl-L-Lys 37% of the activity relative to L-Lys Aeromonas caviae N6-Acetyl-D-Lys
-
?
5.1.1.10 S-Methyl-L-Cys 43% of the activity relative to L-Lys Aeromonas caviae S-Methyl-D-Cys
-
?

Subunits

EC Number Subunits Comment Organism
5.1.1.10 dimer 2 * 40000, SDS-PAGE Aeromonas caviae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.10 60
-
-
Aeromonas caviae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.10 60
-
stable up to 60°C, pH 8.0, 10 min, in 0.1 M potassium phosphate Aeromonas caviae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.10 7.5 9
-
Aeromonas caviae

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.10 pyridoxal 5'-phosphate holoenzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme Aeromonas caviae
5.1.1.10 pyridoxal 5'-phosphate required for maximal activity Aeromonas caviae
5.1.1.10 pyridoxal 5'-phosphate Km: 0.0026 mM Aeromonas caviae