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Literature summary extracted from

  • Tomkinson, A.E.; Levin, D.S.
    Mammalian DNA ligases (1997), BioEssays, 19, 893-901.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.5.1.1 XRCC1 DNA ligase III-alpha forms a complex with the DNA single-strand break repair protein XRCC1 Mammalia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 DNA ligase I, III-alpha, III-beta, IV Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 ATP inhibits the last step of the reaction, which involves cleavage of the diphosphate bond of the DNA-adenylate intermediate and phosphodiester bond formation Mammalia
6.5.1.1 pyridoxal 5'-phosphate inhibits last step of the reaction, which involves cleavage of the diphosphate bond of the DNA-adenylate intermediate and phosphodiester bond formation Mammalia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.5.1.1 nucleus
-
 Drosophila melanogaster 5634
-
6.5.1.1 nucleus
-
 Mammalia 5634
-
6.5.1.1 nucleus
-
 Homo sapiens 5634
-
6.5.1.1 nucleus
-
 Bos taurus 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.1 125000
-
 x * 125000, denaturing PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Drosophila melanogaster
-
 ?
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Homo sapiens
-
 ?
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Saccharomyces cerevisiae
-
 ?
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Bos taurus
-
 ?
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Vaccinia virus
-
 ?
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Mammalia DNA ligase III-beta is expressed only in male meiotic germ cells, suggesting a role for this isoform in meiotic recombination ?
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Mammalia DNA ligase I is involved in DNA replication ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Bos taurus
-
-
-
6.5.1.1 Drosophila melanogaster
-
-
-
6.5.1.1 Homo sapiens
-
-
-
6.5.1.1 Mammalia
-
 DNA ligase I, II, III-alpha, III-beta, and IV. DNA ligase II appears to be derived from DNA ligase III by a proteolytic mechanism
-
6.5.1.1 Saccharomyces cerevisiae
-
-
-
6.5.1.1 Schizosaccharomyces pombe
-
-
-
6.5.1.1 Vaccinia virus
-
-
-
6.5.1.1 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 DNA ligase I Bos taurus
6.5.1.1 DNA ligase I, II, III, and IV Mammalia

Reaction

EC Number Reaction Comment Organism Reaction ID
6.5.1.1 ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate mechanism: 1. formation of a covalent enzyme-adenylate complex, the ATP is cleaved to AMP and diphosphate with adenylyl residue linked by a phosphoramidate bond to the epsilon-amino group of a specific lysine residue at the active site of the protein, 2. the activated AMP residue of the DNA ligase-adenylate intermediate is transferred to the 5'-phosphate terminus of a single strand break in double-stranded DNA to generate a covalent DNA-AMP complex with a 5'-5' phosphoanhydride bond, 3. in the final step of DNA ligation, unadenylated DNA ligase is required for the generation of a phosphodiester bond and catalyzes displacement of the AMP residue through attack by adjacent 3'-hydroxyl group of the adenylylated site Mammalia
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.5.1.1 germ male meiotic cell, ligase III-beta is expressed only in male meiotic germ cells Mammalia
-
6.5.1.1 testis
-
 Homo sapiens
-
6.5.1.1 testis
-
 Bos taurus
-
6.5.1.1 thymus
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Mammalia AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Homo sapiens AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Saccharomyces cerevisiae AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Bos taurus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Xenopus laevis AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Schizosaccharomyces pombe AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Vaccinia virus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Drosophila melanogaster ?
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Homo sapiens ?
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Saccharomyces cerevisiae ?
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Bos taurus ?
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Vaccinia virus ?
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase III-beta is expressed only in male meiotic germ cells, suggesting a role for this isoform in meiotic recombination Mammalia ?
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase I is involved in DNA replication Mammalia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.1 ?
-
 Drosophila melanogaster
6.5.1.1 ?
-
 Homo sapiens
6.5.1.1 ?
-
 Saccharomyces cerevisiae
6.5.1.1 ?
-
 Schizosaccharomyces pombe
6.5.1.1 ? x * 125000, denaturing PAGE Bos taurus