BRENDA - Enzyme Database

Acyl-coenzyme A carboxylases. Homologous 3-methylcrotonyl-CoA and geranyl-CoA carboxylase from Pseudomonas citronellolis

Fall, R.R.; Hector, M.L.; Biochemistry 16, 4000-4005 (1977) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.4
Avidin
irreversible inhibition
Pseudomonas citronellolis
6.4.1.5
Avidin
irreversible
Pseudomonas citronellolis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.4
63000
-
x * 63000 + x * 73000, SDS-PAGE
Pseudomonas citronellolis
6.4.1.4
73000
-
x * 63000 + x * 73000, SDS-PAGE
Pseudomonas citronellolis
6.4.1.4
520000
580000
gel filtration
Pseudomonas citronellolis
6.4.1.5
63000
-
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE
Pseudomonas citronellolis
6.4.1.5
75000
-
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE
Pseudomonas citronellolis
6.4.1.5
520000
580000
gel filtration, native PAGE
Pseudomonas citronellolis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
6.4.1.4
Pseudomonas citronellolis
-
-
-
6.4.1.5
Pseudomonas citronellolis
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.4.1.4
-
Pseudomonas citronellolis
6.4.1.5
-
Pseudomonas citronellolis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4.1.4
10.54
-
-
Pseudomonas citronellolis
6.4.1.5
8.38
-
-
Pseudomonas citronellolis
Storage Stability
EC Number
Storage Stability
Organism
6.4.1.5
-80°C, stable for several months
Pseudomonas citronellolis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6.4.1.4
ATP + 3-methylcrotonoyl-CoA + HCO3-
-
1888
Pseudomonas citronellolis
ADP + phosphate + 3-methylglutaconyl-CoA
-
1888
Pseudomonas citronellolis
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
-
1888
Pseudomonas citronellolis
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
1888
Pseudomonas citronellolis
?
6.4.1.5
additional information
ATP-dependent carboxylation of d-biotin at 3-5% the rate of geranoyl-CoA carboxylation
1888
Pseudomonas citronellolis
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
6.4.1.4
?
x * 63000 + x * 73000, SDS-PAGE
Pseudomonas citronellolis
6.4.1.5
octamer
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE
Pseudomonas citronellolis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.4.1.4
biotin
enzyme contains biotin
Pseudomonas citronellolis
6.4.1.4
biotin
contains 1 mol biotin per 157000 MW AB protomeric structure
Pseudomonas citronellolis
6.4.1.5
biotin
enzyme contains 1 mol of biotin per 148000-157000 g of protein
Pseudomonas citronellolis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.4.1.4
biotin
enzyme contains biotin
Pseudomonas citronellolis
6.4.1.4
biotin
contains 1 mol biotin per 157000 MW AB protomeric structure
Pseudomonas citronellolis
6.4.1.5
biotin
enzyme contains 1 mol of biotin per 148000-157000 g of protein
Pseudomonas citronellolis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.4
Avidin
irreversible inhibition
Pseudomonas citronellolis
6.4.1.5
Avidin
irreversible
Pseudomonas citronellolis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.4
63000
-
x * 63000 + x * 73000, SDS-PAGE
Pseudomonas citronellolis
6.4.1.4
73000
-
x * 63000 + x * 73000, SDS-PAGE
Pseudomonas citronellolis
6.4.1.4
520000
580000
gel filtration
Pseudomonas citronellolis
6.4.1.5
63000
-
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE
Pseudomonas citronellolis
6.4.1.5
75000
-
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE
Pseudomonas citronellolis
6.4.1.5
520000
580000
gel filtration, native PAGE
Pseudomonas citronellolis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.4.1.4
-
Pseudomonas citronellolis
6.4.1.5
-
Pseudomonas citronellolis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4.1.4
10.54
-
-
Pseudomonas citronellolis
6.4.1.5
8.38
-
-
Pseudomonas citronellolis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
6.4.1.5
-80°C, stable for several months
Pseudomonas citronellolis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6.4.1.4
ATP + 3-methylcrotonoyl-CoA + HCO3-
-
1888
Pseudomonas citronellolis
ADP + phosphate + 3-methylglutaconyl-CoA
-
1888
Pseudomonas citronellolis
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
-
1888
Pseudomonas citronellolis
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
1888
Pseudomonas citronellolis
?
6.4.1.5
additional information
ATP-dependent carboxylation of d-biotin at 3-5% the rate of geranoyl-CoA carboxylation
1888
Pseudomonas citronellolis
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.4.1.4
?
x * 63000 + x * 73000, SDS-PAGE
Pseudomonas citronellolis
6.4.1.5
octamer
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE
Pseudomonas citronellolis