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Literature summary extracted from

  • Fall, R.R.
    3-Methylcrotonyl-CoA and geranyl-CoA carboxylases from Pseudomonas citronellolis (1981), Methods Enzymol., 71, 791-799.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.4 0.043
-
3-methylcrotonoyl-CoA
-
Pseudomonas citronellolis
6.4.1.4 0.138
-
(E)-crotonoyl-CoA
-
Pseudomonas citronellolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.4 63000
-
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B Pseudomonas citronellolis
6.4.1.4 73000
-
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B Pseudomonas citronellolis
6.4.1.4 520000 580000 gel filtration Pseudomonas citronellolis
6.4.1.5 63000
-
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE Pseudomonas citronellolis
6.4.1.5 75000
-
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE Pseudomonas citronellolis
6.4.1.5 520000 580000 gel filtration, native PAGE Pseudomonas citronellolis

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.4 Pseudomonas citronellolis
-
-
-
6.4.1.5 Pseudomonas citronellolis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.4
-
Pseudomonas citronellolis
6.4.1.5
-
Pseudomonas citronellolis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.4 10.54
-
-
Pseudomonas citronellolis
6.4.1.5 8.38
-
-
Pseudomonas citronellolis

Storage Stability

EC Number Storage Stability Organism
6.4.1.4 -80°C, approximately 25% loss of activity per month Pseudomonas citronellolis
6.4.1.5 -80°C, stable for several months Pseudomonas citronellolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.4 ATP + 3-methylcrotonoyl-CoA + HCO3-
-
Pseudomonas citronellolis ADP + phosphate + 3-methylglutaconyl-CoA
-
?
6.4.1.4 ATP + crotonoyl-CoA + HCO3- (E)-crotonoyl-CoA Pseudomonas citronellolis ?
-
?
6.4.1.4 ATP + crotonoyl-CoA + HCO3- 44% of the activity relative to 3-methylcrotonoyl-CoA Pseudomonas citronellolis ?
-
?
6.4.1.5 ATP + 3-methylcrotonyl-CoA + HCO3- 4-8% of the activity relative to (Z)-geranoyl-CoA Pseudomonas citronellolis ?
-
?
6.4.1.5 ATP + ethylcrotonyl-CoA + HCO3- cis-ethylcrotonyl-CoA and trans-ethylcrotonyl-CoA Pseudomonas citronellolis ?
-
?
6.4.1.5 ATP + farnesyl-CoA + HCO3- 36% of the activity relative to (Z)-geranoyl-CoA Pseudomonas citronellolis ?
-
?
6.4.1.5 ATP + geranoyl-CoA + HCO3- (Z)-geranoyl-CoA, no activity with (E)-geranoyl-CoA Pseudomonas citronellolis ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
?
6.4.1.5 additional information the enzyme utilizes 5-15-carbon acyl-CoA substrates Pseudomonas citronellolis ?
-
?

Subunits

EC Number Subunits Comment Organism
6.4.1.4 octamer 4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B Pseudomonas citronellolis
6.4.1.5 octamer 4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE Pseudomonas citronellolis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.4.1.5 60
-
50% loss of activity after 1 min Pseudomonas citronellolis

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.4 biotin enzyme contains biotin Pseudomonas citronellolis
6.4.1.4 biotin biotin is bound to the heavier subunit Pseudomonas citronellolis
6.4.1.5 biotin enzyme contains 1 mol of biotin per 148000-157000 g of protein Pseudomonas citronellolis