BRENDA - Enzyme Database

3-Methylcrotonyl-CoA and geranyl-CoA carboxylases from Pseudomonas citronellolis

Fall, R.R.; Methods Enzymol. 71, 791-799 (1981) View publication on PubMed

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.4
0.043
-
3-methylcrotonoyl-CoA
-
Pseudomonas citronellolis
6.4.1.4
0.138
-
(E)-crotonoyl-CoA
-
Pseudomonas citronellolis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.4
63000
-
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B
Pseudomonas citronellolis
6.4.1.4
73000
-
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B
Pseudomonas citronellolis
6.4.1.4
520000
580000
gel filtration
Pseudomonas citronellolis
6.4.1.5
63000
-
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE
Pseudomonas citronellolis
6.4.1.5
75000
-
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE
Pseudomonas citronellolis
6.4.1.5
520000
580000
gel filtration, native PAGE
Pseudomonas citronellolis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
6.4.1.4
Pseudomonas citronellolis
-
-
-
6.4.1.5
Pseudomonas citronellolis
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.4.1.4
-
Pseudomonas citronellolis
6.4.1.5
-
Pseudomonas citronellolis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4.1.4
10.54
-
-
Pseudomonas citronellolis
6.4.1.5
8.38
-
-
Pseudomonas citronellolis
Storage Stability
EC Number
Storage Stability
Organism
6.4.1.4
-80°C, approximately 25% loss of activity per month
Pseudomonas citronellolis
6.4.1.5
-80°C, stable for several months
Pseudomonas citronellolis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6.4.1.4
ATP + 3-methylcrotonoyl-CoA + HCO3-
-
1886
Pseudomonas citronellolis
ADP + phosphate + 3-methylglutaconyl-CoA
-
1886
Pseudomonas citronellolis
?
6.4.1.4
ATP + crotonoyl-CoA + HCO3-
(E)-crotonoyl-CoA
1886
Pseudomonas citronellolis
?
-
-
-
?
6.4.1.4
ATP + crotonoyl-CoA + HCO3-
44% of the activity relative to 3-methylcrotonoyl-CoA
1886
Pseudomonas citronellolis
?
-
-
-
?
6.4.1.5
ATP + 3-methylcrotonyl-CoA + HCO3-
4-8% of the activity relative to (Z)-geranoyl-CoA
1886
Pseudomonas citronellolis
?
-
-
-
?
6.4.1.5
ATP + ethylcrotonyl-CoA + HCO3-
cis-ethylcrotonyl-CoA and trans-ethylcrotonyl-CoA
1886
Pseudomonas citronellolis
?
-
-
-
?
6.4.1.5
ATP + farnesyl-CoA + HCO3-
36% of the activity relative to (Z)-geranoyl-CoA
1886
Pseudomonas citronellolis
?
-
-
-
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
(Z)-geranoyl-CoA, no activity with (E)-geranoyl-CoA
1886
Pseudomonas citronellolis
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
1886
Pseudomonas citronellolis
?
6.4.1.5
additional information
the enzyme utilizes 5-15-carbon acyl-CoA substrates
1886
Pseudomonas citronellolis
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
6.4.1.4
octamer
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B
Pseudomonas citronellolis
6.4.1.5
octamer
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE
Pseudomonas citronellolis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.4.1.5
60
-
50% loss of activity after 1 min
Pseudomonas citronellolis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.4.1.4
biotin
enzyme contains biotin
Pseudomonas citronellolis
6.4.1.4
biotin
biotin is bound to the heavier subunit
Pseudomonas citronellolis
6.4.1.5
biotin
enzyme contains 1 mol of biotin per 148000-157000 g of protein
Pseudomonas citronellolis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.4.1.4
biotin
enzyme contains biotin
Pseudomonas citronellolis
6.4.1.4
biotin
biotin is bound to the heavier subunit
Pseudomonas citronellolis
6.4.1.5
biotin
enzyme contains 1 mol of biotin per 148000-157000 g of protein
Pseudomonas citronellolis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.4
0.043
-
3-methylcrotonoyl-CoA
-
Pseudomonas citronellolis
6.4.1.4
0.138
-
(E)-crotonoyl-CoA
-
Pseudomonas citronellolis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.4
63000
-
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B
Pseudomonas citronellolis
6.4.1.4
73000
-
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B
Pseudomonas citronellolis
6.4.1.4
520000
580000
gel filtration
Pseudomonas citronellolis
6.4.1.5
63000
-
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE
Pseudomonas citronellolis
6.4.1.5
75000
-
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE
Pseudomonas citronellolis
6.4.1.5
520000
580000
gel filtration, native PAGE
Pseudomonas citronellolis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.4.1.4
-
Pseudomonas citronellolis
6.4.1.5
-
Pseudomonas citronellolis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4.1.4
10.54
-
-
Pseudomonas citronellolis
6.4.1.5
8.38
-
-
Pseudomonas citronellolis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
6.4.1.4
-80°C, approximately 25% loss of activity per month
Pseudomonas citronellolis
6.4.1.5
-80°C, stable for several months
Pseudomonas citronellolis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6.4.1.4
ATP + 3-methylcrotonoyl-CoA + HCO3-
-
1886
Pseudomonas citronellolis
ADP + phosphate + 3-methylglutaconyl-CoA
-
1886
Pseudomonas citronellolis
?
6.4.1.4
ATP + crotonoyl-CoA + HCO3-
(E)-crotonoyl-CoA
1886
Pseudomonas citronellolis
?
-
-
-
?
6.4.1.4
ATP + crotonoyl-CoA + HCO3-
44% of the activity relative to 3-methylcrotonoyl-CoA
1886
Pseudomonas citronellolis
?
-
-
-
?
6.4.1.5
ATP + 3-methylcrotonyl-CoA + HCO3-
4-8% of the activity relative to (Z)-geranoyl-CoA
1886
Pseudomonas citronellolis
?
-
-
-
?
6.4.1.5
ATP + ethylcrotonyl-CoA + HCO3-
cis-ethylcrotonyl-CoA and trans-ethylcrotonyl-CoA
1886
Pseudomonas citronellolis
?
-
-
-
?
6.4.1.5
ATP + farnesyl-CoA + HCO3-
36% of the activity relative to (Z)-geranoyl-CoA
1886
Pseudomonas citronellolis
?
-
-
-
?
6.4.1.5
ATP + geranoyl-CoA + HCO3-
(Z)-geranoyl-CoA, no activity with (E)-geranoyl-CoA
1886
Pseudomonas citronellolis
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
1886
Pseudomonas citronellolis
?
6.4.1.5
additional information
the enzyme utilizes 5-15-carbon acyl-CoA substrates
1886
Pseudomonas citronellolis
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.4.1.4
octamer
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B
Pseudomonas citronellolis
6.4.1.5
octamer
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE
Pseudomonas citronellolis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.4.1.5
60
-
50% loss of activity after 1 min
Pseudomonas citronellolis