EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.4 | 0.043 | - |
3-methylcrotonoyl-CoA | - |
Pseudomonas citronellolis | |
6.4.1.4 | 0.138 | - |
(E)-crotonoyl-CoA | - |
Pseudomonas citronellolis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.4.1.4 | 63000 | - |
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B | Pseudomonas citronellolis |
6.4.1.4 | 73000 | - |
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B | Pseudomonas citronellolis |
6.4.1.4 | 520000 | 580000 | gel filtration | Pseudomonas citronellolis |
6.4.1.5 | 63000 | - |
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE | Pseudomonas citronellolis |
6.4.1.5 | 75000 | - |
4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE | Pseudomonas citronellolis |
6.4.1.5 | 520000 | 580000 | gel filtration, native PAGE | Pseudomonas citronellolis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.4.1.4 | Pseudomonas citronellolis | - |
- |
- |
6.4.1.5 | Pseudomonas citronellolis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.4.1.4 | - |
Pseudomonas citronellolis |
6.4.1.5 | - |
Pseudomonas citronellolis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.4.1.4 | 10.54 | - |
- |
Pseudomonas citronellolis |
6.4.1.5 | 8.38 | - |
- |
Pseudomonas citronellolis |
EC Number | Storage Stability | Organism |
---|---|---|
6.4.1.4 | -80°C, approximately 25% loss of activity per month | Pseudomonas citronellolis |
6.4.1.5 | -80°C, stable for several months | Pseudomonas citronellolis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.4 | ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Pseudomonas citronellolis | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
6.4.1.4 | ATP + crotonoyl-CoA + HCO3- | (E)-crotonoyl-CoA | Pseudomonas citronellolis | ? | - |
? | |
6.4.1.4 | ATP + crotonoyl-CoA + HCO3- | 44% of the activity relative to 3-methylcrotonoyl-CoA | Pseudomonas citronellolis | ? | - |
? | |
6.4.1.5 | ATP + 3-methylcrotonyl-CoA + HCO3- | 4-8% of the activity relative to (Z)-geranoyl-CoA | Pseudomonas citronellolis | ? | - |
? | |
6.4.1.5 | ATP + ethylcrotonyl-CoA + HCO3- | cis-ethylcrotonyl-CoA and trans-ethylcrotonyl-CoA | Pseudomonas citronellolis | ? | - |
? | |
6.4.1.5 | ATP + farnesyl-CoA + HCO3- | 36% of the activity relative to (Z)-geranoyl-CoA | Pseudomonas citronellolis | ? | - |
? | |
6.4.1.5 | ATP + geranoyl-CoA + HCO3- | (Z)-geranoyl-CoA, no activity with (E)-geranoyl-CoA | Pseudomonas citronellolis | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? | |
6.4.1.5 | additional information | the enzyme utilizes 5-15-carbon acyl-CoA substrates | Pseudomonas citronellolis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.4.1.4 | octamer | 4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B | Pseudomonas citronellolis |
6.4.1.5 | octamer | 4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE | Pseudomonas citronellolis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.5 | 60 | - |
50% loss of activity after 1 min | Pseudomonas citronellolis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.4 | biotin | enzyme contains biotin | Pseudomonas citronellolis | |
6.4.1.4 | biotin | biotin is bound to the heavier subunit | Pseudomonas citronellolis | |
6.4.1.5 | biotin | enzyme contains 1 mol of biotin per 148000-157000 g of protein | Pseudomonas citronellolis |