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Literature summary extracted from

  • Henrikson, K.P.; Allen, S.H.G.
    Purification and subunit structure of propionyl coenzyme A carboxylase of Mycobacterium smegmatis (1979), J. Biol. Chem., 254, 5888-5891.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.3 0.035
-
 propanoyl-CoA
-
 Mycolicibacterium smegmatis
6.4.1.3 0.05
-
 acetyl-CoA
-
 Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.3 57000
-
 x * 57000 + x * 64000, SDS-PAGE of mercaptoethanol-treated enzyme Mycolicibacterium smegmatis
6.4.1.3 64000
-
 x * 57000 + x * 64000, SDS-PAGE of mercaptoethanol-treated enzyme Mycolicibacterium smegmatis
6.4.1.3 500000
-
 PAGE, gel filtration, sucrose density gradient ultracentrifugation Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.3 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.3
-
 Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.3 9.7
-
-
 Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.3 ATP + acetyl-CoA + HCO3- no other acetyl-CoA carboxylase detected in this organism Mycolicibacterium smegmatis ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Mycolicibacterium smegmatis ADP + phosphate + methylmalonyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.3 ? x * 57000 + x * 64000, SDS-PAGE of mercaptoethanol-treated enzyme Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.3 biotin biotin-containing enzyme Mycolicibacterium smegmatis
6.4.1.3 biotin 4 mol of biotin bound per mol of enzyme Mycolicibacterium smegmatis
6.4.1.3 biotin 3.8 mol of biotin per mol of enzyme Mycolicibacterium smegmatis